Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,546,287 T→C 6.0% intergenic (‑34/+64) rutR_2 ← / ← CJ019_04309 HTH‑type transcriptional regulator RutR/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,546,2870TC 6.0% 219.8 / 3.5 67intergenic (‑34/+64)rutR_2/CJ019_04309HTH‑type transcriptional regulator RutR/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (38/25);  new base C (2/2);  total (40/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

AGCAGGTCCTGCAGCACCGCGCGGAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATAACCGTCAGGGTCGATGAAATGAAAGCGCCGCCCGCCG  >  KT2440/4546153‑4546420
                                                                                                                                      |                                                                                                                                     
agcagGTCCTGCAGCACCGCGCGGAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCt                                                                                                                                       >  8:353513/1‑135 (MQ=255)
agcagGTCCTGCAGCACCGCGCGGAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCt                                                                                                                                       >  8:221273/1‑135 (MQ=255)
   agGTCCTGCAGCACCGCGCGGAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTc                                                                                                                                   >  4:222470/1‑136 (MQ=255)
    ggTCCTGCAGCACCGCGCGGAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCCTCGCGCAACCCCCTTTTcc                                                                                                                                  >  4:8726/1‑136 (MQ=255)
         tGCAGCACCGCGCGGAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTccgccg                                                                                                                              >  7:170905/1‑135 (MQ=255)
           cagcaCCGCGCGGAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCCTTTCCGCCGCtt                                                                                                                           >  4:44419/1‑136 (MQ=255)
           cagcaCCGCGCGGAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCCTTTCCGCCGCtt                                                                                                                           >  4:49200/1‑136 (MQ=255)
             gcaCCGCGCGGAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACCCCTTTTCCGCCGCTTg                                                                                                                          >  1:183635/1‑135 (MQ=255)
              caCCGCGCGGAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTAGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCtt                                                                                                                           >  5:298027/1‑133 (MQ=255)
                   cgtggAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGAtt                                                                                                                   >  3:170071/4‑136 (MQ=255)
                   cgcgGAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGAt                                                                                                                    >  6:326191/1‑135 (MQ=255)
                      ggAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGAttttt                                                                                                                >  4:71777/1‑136 (MQ=255)
                      ggAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGAttttt                                                                                                                >  4:170500/1‑136 (MQ=255)
                      ggAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGAttttt                                                                                                                >  4:83142/1‑136 (MQ=255)
                      ggAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGAtttt                                                                                                                 >  5:344795/1‑135 (MQ=255)
                      ggAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGAtttt                                                                                                                 >  1:69571/1‑135 (MQ=255)
                                gTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCTGGATTTTTTCGATATTg                                                                                                       >  6:324933/1‑135 (MQ=255)
                                gTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGc                                                                                                      >  3:84845/1‑136 (MQ=255)
                                  ccTTGGTGCTGAATTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGACCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACCACTTTTCCGCCGCTTTCAGGATGTTTTCGATATTGCg                                                                                                     >  5:160125/1‑135 (MQ=255)
                                     tGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTcg                                                                                                  >  8:106230/1‑135 (MQ=255)
                                           tGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGAt                                                                                            <  8:170905/135‑1 (MQ=255)
                                           tGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTAGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGa                                                                                             <  6:298027/134‑1 (MQ=255)
                                               gTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGa                                                                                       >  3:29023/1‑136 (MQ=255)
                                                taataaTGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGAt                                                                                      >  4:248023/1‑136 (MQ=255)
                                                     atGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTtg                                                                                  >  5:4331/1‑135 (MQ=255)
                                                         aGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGa                                                                               <  1:23859/134‑1 (MQ=255)
                                                            ttGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  3:83142/136‑1 (MQ=255)
                                                            ttGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCCTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  3:44419/136‑1 (MQ=255)
                                                            ttGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGACCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCCTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGGCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  3:49200/136‑1 (MQ=255)
                                                             tGGAGCGTGGCAGCTGAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  6:329209/135‑1 (MQ=255)
                                                             tGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTgg                                                                           >  8:67028/1‑134 (MQ=255)
                                                             tGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTgg                                                                           >  7:306530/1‑134 (MQ=255)
                                                             tGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  5:145254/135‑1 (MQ=255)
                                                             tGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  6:4331/135‑1 (MQ=255)
                                                             tGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  5:258691/135‑1 (MQ=255)
                                                             tGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  2:69571/135‑1 (MQ=255)
                                                             tGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  2:50348/135‑1 (MQ=255)
                                                             tGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  8:306530/135‑1 (MQ=255)
                                                             tGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  5:69210/135‑1 (MQ=255)
                                                             tGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGAAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  7:267379/135‑1 (MQ=255)
                                                                      gCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCt                                                                 <  6:242998/135‑1 (MQ=255)
                                                                        aGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          >  5:29197/1‑124 (MQ=255)
                                                                        aGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGc                                                                          <  6:29197/124‑1 (MQ=255)
                                                                        aGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTg                                                               >  2:19853/1‑135 (MQ=255)
                                                                         gCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTggg                                                             <  3:71777/136‑1 (MQ=255)
                                                                             aGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGa                                                                               >  5:366199/1‑114 (MQ=255)
                                                                             aGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGa                                                                               <  6:366199/114‑1 (MQ=255)
                                                                             aGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCg                                                          >  6:109737/1‑135 (MQ=255)
                                                                               ccTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTc                                                        <  7:353513/135‑1 (MQ=255)
                                                                                      ggCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATAGGCCATCAGGCTTGGGCCGGCCACACCg                                                 >  5:7664/1‑135 (MQ=255)
                                                                                               gCCCATGGAGGTGGCGCCGTAGCCCCTCTCGCAAACACCTTTTCAGCCGCTTGCAGGATTTTTTCGCTATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCGTGGGCCGTCCACACCGCCAGCt                                           <  3:8726/132‑1 (MQ=255)
                                                                                                cccATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATa                                       >  1:23036/1‑135 (MQ=255)
                                                                                                   aTGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATAAcc                                    <  2:23036/135‑1 (MQ=255)
                                                                                                       aGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATAACCGTCa                                <  5:326191/135‑1 (MQ=255)
                                                                                                       aGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATAACCGTCAg                               >  4:147839/1‑136 (MQ=255)
                                                                                                        ggTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATAACCGTCAg                               >  1:295842/1‑135 (MQ=255)
                                                                                                            gcgcCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATAACCGTCAGGGt                            >  2:210958/1‑134 (MQ=255)
                                                                                                              gcCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGTCTTGGGCCGTCCCCACCGCCAGCTCATACCCGTCAGGGTCGa                         >  6:216086/1‑135 (MQ=255)
                                                                                                              gcCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATAACCGTCAGGGTCGa                         >  5:82947/1‑135 (MQ=255)
                                                                                                                cGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATAACCGTCAGGGTCGat                        >  2:230773/1‑134 (MQ=255)
                                                                                                                            cGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCAATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATAACCGTCAGGGTCGATGAAATGAAAGcgcc          >  4:180780/1‑136 (MQ=255)
                                                                                                                             gCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTtgtg                                                                                <  7:240196/65‑1 (MQ=255)
                                                                                                                             gCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTtgtg                                                                                >  8:240196/1‑65 (MQ=255)
                                                                                                                             gCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCacac                                                   <  6:89054/94‑1 (MQ=255)
                                                                                                                             gCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCacac                                                   >  5:89054/1‑94 (MQ=255)
                                                                                                                             gCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATAACCGTCAGGGTCGATGAAATGAAAGcgccg         >  3:179470/1‑136 (MQ=255)
                                                                                                                             gCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATAACCGTCAGGGTCGATGAAATGAAAGcgcc          >  8:104775/1‑135 (MQ=255)
                                                                                                                                    ccTTTTCCGCCGCTTGCAGTTTTTTTTCGATATGGCGTCGGCGGATTTCGATCTTGTGATGGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCAAAACCGTCAGGGTCGATGAAATGAAAGCGCCGCccgccg  <  4:29023/136‑1 (MQ=255)
                                                                                                                                     cTTTTCCGCCGCTTGCAGTATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTGGGGCCGTCCACACCGCCAGCTCATAACCGTCAGGGTCGAGGAAATGAAAGCGCCGCccgccg  <  7:221273/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
AGCAGGTCCTGCAGCACCGCGCGGAACAGCTCGTCCTTGGTGCTGAAGTAATAATGCAGGTTGGAGCGTGGCAGCTCAGCCTGCTCGGCGATATCGCCCATGGAGGTGGCGCCGTAGCCCTTCTCGCAAACACCTTTTCCGCCGCTTGCAGGATTTTTTCGATATTGCGTCGGCGGATTTCGATCTTGTGATTGGCCATCAGGCTTGGGCCGTCCACACCGCCAGCTCATAACCGTCAGGGTCGATGAAATGAAAGCGCCGCCCGCCG  >  KT2440/4546153‑4546420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: