Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,647,997	C→T	7.1%	G276G (GGC→GGT)	aceA →	Isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,647,997	0	C	T	7.1%	179.5 / 3.5	56	G276G (GGC→GGT)	aceA	Isocitrate lyase
Reads supporting (aligned to +/- strand):  ref base C (28/24);  new base T (2/2);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

ACCGACCATCATCCTGGCGCGTACCGATGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGTACCCGGACCAGA  >  KT2440/4647868‑4648130                                                                                                                                  |                                                                                                                                      aCCGACCATCATCCTGGGGCGTACCGATGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGa                                                                                                                                  >  5:71912/1‑135 (MQ=255) aCCGACCATCATCCTGGCGCGTACCGATGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCg                                                                                                                                   <  8:19480/134‑1 (MQ=255) aCCGACCATCATCCTGGCGCGTACCGATGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCg                                                                                                                                   <  4:318331/134‑1 (MQ=255) aCCGACCATCATCCTGGCGCGTACCGATGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCg                                                                                                                                   >  3:318331/1‑134 (MQ=255)   cGACCATCATCCTGGCGCGTACCGATGCCAACGCCGCCGCCCTGCTGACCAGCGACTGCGCCCCGTACGACCAGCCGTTAGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGAcc                                                                                                                                >  8:37522/1‑135 (MQ=255)        atcatcCTGGCGCGTACCGATTCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCATCCGCGAAGGCTTCAACAAGGTCCGCGCCGGCCTCGACCAgg                                                                                                                             >  7:90844/1‑133 (MQ=255)                    cgTACCGATGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCggc                                                                                                               >  8:56378/1‑135 (MQ=255)                       aCCGATGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCggcctg                                                                                                            <  7:37522/135‑1 (MQ=255)                        ccGATGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCggcctggc                                                                                                          <  3:364448/136‑1 (MQ=255)                         cGATGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCggcctggc                                                                                                          >  3:280925/1‑135 (MQ=255)                         cGATGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCCCACCCGCGCAGGCTTCAACAAGCTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCggccaggc                                                                                                          >  7:328712/1‑135 (MQ=255)                          gATGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCggcctggcc                                                                                                         <  1:159292/135‑1 (MQ=255)                           aTGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCggcctggcct                                                                                                        <  5:339998/135‑1 (MQ=255)                              ccAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTAcc                                                                                                     >  5:105180/1‑134 (MQ=255)                                    ccgccgACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGGTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGt                                                                                               <  6:305300/135‑1 (MQ=255)                                         gACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGcc                                                                                          <  1:361033/135‑1 (MQ=255)                                         gACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGcc                                                                                          <  8:321618/135‑1 (MQ=255)                                                         aCTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCg                                                                          >  4:112913/1‑135 (MQ=255)                                                            gCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCAGATCTGGTGAGaaa                                                                       >  7:347354/1‑135 (MQ=255)                                                               aCCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCg                                                                    <  1:6003/135‑1 (MQ=255)                                                                 ccGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCa                                                                 <  3:188355/136‑1 (MQ=255)                                                                       gACCAGCCGTTCGAGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGCCCCCGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCAGATCTGGTGCGCAACCGCCAAGCCg                                                            >  8:172602/1‑135 (MQ=255)                                                                               gTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTCCGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGa                                                    >  7:151564/1‑135 (MQ=255)                                                                               gTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAACCCGCCACGCCGGACCTggc                                                    >  6:249373/1‑134 (MQ=255)                                                                               gTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGAc                                                   >  3:102015/1‑136 (MQ=255)                                                                                 tCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGCCCTGATCTGGTGCGAAACCGCCAAGCCGGCCCTggcc                                                   >  6:294117/1‑132 (MQ=255)                                                                                       ttGGCGAGCGCACCCGCGAAGGCTTCTATAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCg                                           <  3:367448/136‑1 (MQ=255)                                                                                       ttGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGTCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGccc                                            >  8:301984/1‑135 (MQ=255)                                                                                       ttGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGTCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGccc                                            >  8:306949/1‑135 (MQ=255)                                                                                        tGGCTAGCGCACCCGAGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCACGCCGGACCTGGACGAAGCCCg                                           <  7:350663/135‑1 (MQ=255)                                                                                        tGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCg                                           <  7:64496/135‑1 (MQ=255)                                                                                        tGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCg                                           <  6:71912/135‑1 (MQ=255)                                                                                                  aCCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCg                                >  3:365601/1‑136 (MQ=255)                                                                                                    acgcgAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCAGTGCCCGCGGCCTTGCCTACGCCCCGTACGCCGACCTGAGCTGGGGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGa                               <  7:119833/134‑1 (MQ=255)                                                                                                       cgAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGCCCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGa                               >  2:295033/1‑132 (MQ=255)                                                                                                       cgAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGAGGc                            <  8:239672/135‑1 (MQ=255)                                                                                                           ggCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGAGGCGATc                        <  8:328712/135‑1 (MQ=255)                                                                                                                 tACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCGTCGCCGCGGCGAGCAAGAAgg                 >  3:194166/1‑136 (MQ=255)                                                                                                                 tACAACGTCCGAGCCGGCCTCGACCATGCCATTGCCCGCGGCCTGGCCTACGCCCCCTACGCCGACCTGATCTGGTGCGAAACCGCCAACCCGGACCTCGACGAAGCCAGTCGCTTCGCCGATGCGATCaagaat                  >  1:347471/1‑134 (MQ=255)                                                                                                                     aGGTCCGCGCCGGTCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGt              <  7:301984/135‑1 (MQ=255)                                                                                                                     aGGTCCGCGCCGGTCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGt              <  7:306949/135‑1 (MQ=255)                                                                                                                         ccGCGCCGGCCTCGACCAGGCCATTGCCCGCggcctggcct                                                                                                        <  1:168829/41‑1 (MQ=255)                                                                                                                         ccGCGCCGGCCTCGACCAGGCCATTGCCCGCggcctggcct                                                                                                        >  2:168829/1‑41 (MQ=255)                                                                                                                         ccGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGCCCTGGACGAAGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGTACCCg         >  4:88133/1‑136 (MQ=255)                                                                                                                         ccGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGTACCCg         >  4:92903/1‑136 (MQ=255)                                                                                                                           gcgcCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAAcc                                                                     >  6:19748/1‑74 (MQ=255)                                                                                                                           gcgcCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAAcc                                                                     <  5:19748/74‑1 (MQ=255)                                                                                                                             gcCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGCCCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGCCGCAGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGTACCCGGAc      >  7:260329/1‑135 (MQ=255)                                                                                                                             gcCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGCCCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGTACCCGGAcc     >  3:126710/1‑136 (MQ=255)                                                                                                                             gcCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAAc                                                                      <  2:228971/71‑1 (MQ=255)                                                                                                                             gcCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAAc                                                                      >  1:228971/1‑71 (MQ=255)                                                                                                                             gcCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCACGCCGGCCCTGGACGCCGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGTACCCggcc      >  8:215688/1‑133 (MQ=255)                                                                                                                             gcCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGTACCCGGAcc     >  3:120705/1‑136 (MQ=255)                                                                                                                                ggCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGTACCCGGACCAGa  <  3:112913/136‑1 (MQ=255)                                                                                                                                 gCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGTCGAAGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGTACCCGGACCAGa  <  8:8795/135‑1 (MQ=255)                                                                                                                                 gCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGTACCCGGACCAGa  <  8:200432/135‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      ACCGACCATCATCCTGGCGCGTACCGATGCCAACGCCGCCGACCTGCTGACCAGCGACTGCGACCCGTACGACCAGCCGTTCGTGGTTGGCGAGCGCACCCGCGAAGGCTTCTACAAGGTCCGCGCCGGCCTCGACCAGGCCATTGCCCGCGGCCTGGCCTACGCCCCGTACGCCGACCTGATCTGGTGCGAAACCGCCAAGCCGGACCTGGACGAAGCCCGTCGCTTCGCCGAGGCGATCAAGAAGGAGTACCCGGACCAGA  >  KT2440/4647868‑4648130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑