Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,744,948 T→A 5.8% I291F (ATC→TTC)  proP_5 ← Proline/betaine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,744,9480TA 5.8% 221.8 / 3.5 69I291F (ATC→TTC) proP_5Proline/betaine transporter
Reads supporting (aligned to +/- strand):  ref base T (27/38);  new base A (2/2);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

GCCCCACCAGGCTGGCGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAGGTCAGCACACAGGTCAGCAACGCAGCAGGGATCA  >  KT2440/4744816‑4745079
                                                                                                                                    |                                                                                                                                   
gccccacCAGGCTGGCGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGcc                                                                                                                                  >  3:356276/1‑136 (MQ=255)
  cccacCAGGCTGGCGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGccc                                                                                                                                 <  5:132612/135‑1 (MQ=255)
  cccacCAGGCGGGCGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGccc                                                                                                                                 <  5:97604/135‑1 (MQ=255)
    ccccaGGCTGGCGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCAc                                                                                                                               >  6:15529/3‑135 (MQ=255)
      ccaGGCTGGCGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACgct                                                                                                                            <  4:6069/136‑1 (MQ=255)
      ccaGGCTGGCGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACgct                                                                                                                            <  4:192898/136‑1 (MQ=255)
       caGGCTGGCGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACgct                                                                                                                            <  1:138327/135‑1 (MQ=255)
       caGGCTGGCGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACgct                                                                                                                            <  5:95193/135‑1 (MQ=255)
         ggCTGGCGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACgctgc                                                                                                                          <  8:125225/135‑1 (MQ=255)
              gCGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAga                                                                                                                     <  8:268708/135‑2 (MQ=255)
               ggTACAACGCGCCGATACGCAGCGTCAGCAAAAGGCTATACAGCATAAGCGCGCGCCAGGCACCCACACTGTCGACAAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCACTCGCTGCTCa                                                                                                                       <  2:332937/131‑1 (MQ=255)
               cGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACGAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAggg                                                                                                                    <  1:63085/135‑1 (MQ=255)
                 tACAACGCGCCGATACCCAGCGGCAGCTACAGGCTATACAGCTTCAGCGCGCGCCAGGCACCCACCCGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGAAGCCCACGCTGCTCAGGGc                                                                                                                   >  5:316260/1‑134 (MQ=255)
                 tACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGAAGCCCACGCTGCTCAGGGc                                                                                                                   >  5:310742/1‑134 (MQ=255)
                         gcCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAAGGTTGAGGAAGACGCTGCCCACGCTGCTCAGGGCGCAGGtgtg                                                                                                          >  2:78891/1‑135 (MQ=255)
                           cGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGccc                                                                                                       >  3:178215/1‑136 (MQ=255)
                           cGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGcc                                                                                                        >  2:204057/1‑135 (MQ=255)
                                    gCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCa                                                                                               <  7:40151/135‑1 (MQ=255)
                                      ggCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATg                                                                                             >  2:248744/1‑135 (MQ=255)
                                               aGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACgct                                                                                   <  3:76321/136‑1 (MQ=255)
                                                tgCTATAATGCTTCAGCGCGCGCCAGGAACCCACACTGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGATGAAGACGATGCCCACGCTGCTCAGGGAGAAGGTGTGCCCGGCACTCATGCCGAAACgct                                                                                   <  8:359288/134‑1 (MQ=255)
                                                ggCTATACAGCATCAGCGCGCGCCCGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACgcg                                                                                   <  8:359286/135‑2 (MQ=255)
                                                          cATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCAcc                                                                        <  4:178215/136‑1 (MQ=255)
                                                             cAGCGCGCGCCAGGCACCCACCCGGTCTACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGCCGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCCTGCCGAACCGCTGCTGCATCACCGTc                                                                     >  4:79628/1‑136 (MQ=255)
                                                             cAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACgct                                                                                                                            >  2:328385/1‑81 (MQ=255)
                                                             cAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACgct                                                                                                                            <  2:108726/81‑1 (MQ=255)
                                                             cAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACgct                                                                                                                            >  1:108726/1‑81 (MQ=255)
                                                             cAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACgct                                                                                                                            <  1:328385/81‑1 (MQ=255)
                                                             cAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGt                                                                      >  1:365696/1‑135 (MQ=255)
                                                             cAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGt                                                                      >  2:24733/1‑135 (MQ=255)
                                                             cAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGt                                                                      >  2:107036/1‑135 (MQ=255)
                                                             cAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGt                                                                      >  1:63485/1‑135 (MQ=255)
                                                             cAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTc                                                                     >  3:325505/1‑136 (MQ=255)
                                                             cAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTc                                                                     >  3:130217/1‑136 (MQ=255)
                                                             cAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTc                                                                     >  4:101949/1‑136 (MQ=255)
                                                              aGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTc                                                                     >  7:167245/1‑135 (MQ=255)
                                                               gcgcgcgcCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTc                                                                     <  1:68862/134‑1 (MQ=255)
                                                               gcgcgcgcCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCg                                                                    >  2:285356/1‑135 (MQ=255)
                                                                   gcgcCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTg                                                               <  4:217285/136‑1 (MQ=255)
                                                                         ggCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATcacc                                                         <  4:356276/136‑1 (MQ=255)
                                                                          gCACCCACACGCTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATcacc                                                         <  1:81484/135‑1 (MQ=255)
                                                                             cccACACGGTCGACCAGCAGCCCGGCCAGCTCGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGGAACGCTGCTGCATCACCGTCGGCGTGCTCaccccc                                                      >  7:40202/1‑135 (MQ=255)
                                                                                  acGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGcac                                                 >  7:270553/1‑135 (MQ=255)
                                                                                          ccAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCAccgc                                        <  4:259481/136‑1 (MQ=255)
                                                                                          ccAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCAccgc                                        <  3:39645/136‑1 (MQ=255)
                                                                                           cagcagCCCGGCCAGCACGCAGCCACTGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCTCTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCAccgc                                        <  5:30220/135‑1 (MQ=255)
                                                                                              cagcCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGa                                     <  8:350700/135‑1 (MQ=255)
                                                                                              cagcCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGa                                     <  8:270553/135‑1 (MQ=255)
                                                                                              cagcCCGGCCACCACGCAGCCAATGTTGAGGAAGACGATGCCCCCGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGa                                     <  6:21791/135‑1 (MQ=255)
                                                                                               agcCCGGCCAGCACGCAGCCAATGATGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACTCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAgg                                   <  3:129177/136‑1 (MQ=255)
                                                                                                   cGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAGGTCAg                               >  4:331644/1‑136 (MQ=255)
                                                                                                     gCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAGGTCAGc                              <  6:182354/135‑1 (MQ=255)
                                                                                                     gCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAGGTCAGc                              <  5:319207/135‑1 (MQ=255)
                                                                                                     gCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAGGTCAGc                              <  8:167245/135‑1 (MQ=255)
                                                                                                             gCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCCCTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCCCCAGCCCCACCCCCGCCGAGGTCAGCCCACAGGt                      >  6:205251/1‑135 (MQ=255)
                                                                                                             gCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCCCCCGCACCACCACCGCCGAGGTCAGCACACAGGt                      >  1:367720/1‑135 (MQ=255)
                                                                                                             gCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAGGTCAGCACACAGGt                      <  6:130216/135‑1 (MQ=255)
                                                                                                                  cAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGctgctg                                                                               >  4:322077/1‑73 (MQ=255)
                                                                                                                  cAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGctgctg                                                                               <  3:322077/73‑1 (MQ=255)
                                                                                                                       ttGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAGGTCAGCACACAGGTCAGCAACgca            <  3:222166/135‑1 (MQ=255)
                                                                                                                        tGAGGAAGACGATGCCCACGCGGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAGGTCAGCACACAGGTCAGCAACgca            <  2:365696/134‑1 (MQ=255)
                                                                                                                            gAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAGGTCAGCACACAGGTCAGCAACgca            <  1:297390/130‑1 (MQ=255)
                                                                                                                            gAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAGGTCAGCACACAGGTCAGCAACgca            >  2:297390/1‑130 (MQ=255)
                                                                                                                              aGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAgg                                   >  8:112417/1‑105 (MQ=255)
                                                                                                                              aGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAgg                                   <  7:112417/105‑1 (MQ=255)
                                                                                                                                 cGAAGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCCCCGCCGAGGTCAGCACACAGGTCAGCAACGCAGCAGGGATCa  <  6:316260/135‑1 (MQ=255)
                                                                                                                                 cGAAGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAGGTCAGCACACAGGTCAGCAACGCAGCAGGGATCa  <  6:310742/135‑1 (MQ=255)
                                                                                                                                  gATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGa                                     >  2:362801/1‑99 (MQ=255)
                                                                                                                                  gATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGa                                     <  1:362801/99‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
GCCCCACCAGGCTGGCGTACAACGCGCCGATACCCAGCGGCAGCAACAGGCTATACAGCATCAGCGCGCGCCAGGCACCCACACGGTCGACCAGCAGCCCGGCCAGCACGCAGCCAATGTTGAGGAAGACGATGCCCACGCTGCTCAGGGCGAAGGTGTGCCCGGCACTCATGCCGAAACGCTGCTGCATCACCGTCGGCGTGATCACCACCAGCACCACCACCGCCGAGGTCAGCACACAGGTCAGCAACGCAGCAGGGATCA  >  KT2440/4744816‑4745079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑