| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,749,309 | C→G | 24.4% | V301V (GTC→GTG) | macB → | Macrolide export ATP‑binding/permease proteinMacB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,749,309 | 0 | C | G | 24.4% | 58.7 / 2.0 | 39 | V301V (GTC→GTG) | macB | Macrolide export ATP‑binding/permease proteinMacB |
| Reads supporting (aligned to +/- strand): ref base C (9/19); new base G (8/2); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCGCATCACCCTGGACGATGTT > KT2440/4749180‑4749432 | cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTGGGTGGGGGTGTTTTTTGGTGTGGGt > 2:138985/1‑133 (MQ=255)cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGGTGTCGGTg > 4:10580/1‑134 (MQ=255)cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTg > 4:161524/1‑134 (MQ=255)cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTg > 4:80632/1‑134 (MQ=255)cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGAGGTTGGCTGTCGGt > 8:283199/1‑133 (MQ=255)cTGGAAAGGTCAACTGCTCGAATCGCTGCAGGCGGCATGTCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGGGATGTTGGGTGTCGGCGa > 2:241817/1‑135 (MQ=255) taaaGGTGAACTGCTCTAATCGCTGCAGGGGGAATGGCGAGTGAGGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGgc < 4:356496/135‑1 (MQ=255) gAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCaaa < 5:151429/135‑1 (MQ=255) aaCTGCTCGAATCGCTGCAGGCGGCATGGAGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGGGGGGGTGGGGATGTTGGCAGGGGGGGACGGCAGCAAAc > 2:173951/1‑135 (MQ=255) cTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGGGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACg < 8:150790/134‑1 (MQ=255) gCTCGAACCGCTGCAGGCGGCAGGGCGAGGGAGGTGGAGCACCCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCAGCGGAGTCGCGTCGGTGGTGGGGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAg < 3:76637/136‑1 (MQ=255) gAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGGTGTCGGTGCAGGCAGCAAACGCCAGGTa > 6:19643/1‑135 (MQ=255) aTCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATg < 3:80632/136‑1 (MQ=255) aTCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACCGCACTCACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGGGGAGATTTTGGCTGTCGGGGAATGGAGGAAACGCCAGGGAAt > 8:157275/1‑135 (MQ=255) cATGGCGAGTGAGGTGGAGCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGgccg < 1:201244/135‑1 (MQ=255) gaggtgGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCGGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTc < 5:19643/132‑1 (MQ=255) gATGTGGATCACCCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTc < 1:138985/135‑1 (MQ=255) gATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCAGCGGAGTCGCGTCGGTGGTGGTGATGTGGGCGGGCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTc < 2:119583/135‑1 (MQ=255) gATGTGGATCAACCGCTTCCGCACGGCACTGACCCGGCTCGGAATCATCATCGGAGTCGCGGCGGGGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCt < 8:246382/134‑1 (MQ=255) aggtgGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTcc < 4:106107/133‑1 (MQ=255) aTGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGGGGGGGGGATGTTGTGTGTGGGTGGAGGCCCCCAACCCCCGGTCATGGCACAGCTGGCCCCCGATGGCTcc > 2:163746/1‑135 (MQ=255) ggtgGATCCACCGCTTCCGCACGGCACTGACCCTGCTCGGATGCATCCGCGGAGTCGCGTCGGGGGTGGGGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCa < 1:129870/134‑1 (MQ=255) gATCAACGGCTTGCGCACGGAACTGACCCTGCTCGTAATCATCATCGGAGGCGCGTCGGTGGGGGTGATGTTGGCTGTGGGTTAAGGCAGCAAACGCCAGGTTATTGCACAGATGGCCGCCTTCGGCTCCAACATc < 3:103100/136‑1 (MQ=255) aTCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGTGGTGATGTTGGGTGTGGGGGAAGGCAGCCAACGCCAGGTAATGGCACAGATGGCCGCCGTCGGCTCCCACATcc > 3:230944/1‑136 (MQ=255) gCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGTGGTGATGTTGGCTGTGGGGGCAGGCAGCAAACGCCAGGGTATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCt > 3:347033/1‑136 (MQ=255) cTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTGGGGGGGGGGGTTGTTGGGGGTGGGGGAAGGGAGCCAACGCCCGGGAATGGGCCAGATTGCCCCCGTCGGCGCCACCATCCTATAcc > 6:248272/1‑134 (MQ=255) ccGCACGGCACTCACCCTGCTCGGAATCATCATCGGAGTCGCGTGGGGGGTGGTGATGTTGGCTGGCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAAt < 3:10580/136‑1 (MQ=255) cGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGGGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAAt < 5:48338/135‑1 (MQ=255) cACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGGGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATgg < 7:236117/135‑1 (MQ=255) ggCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTTATGTTGGCTGTCGGTGCAGGCAGCAAAAGCCAGGTAATTGCACAGATGGCCGCCTTCGGCTCCACAAACCTAGACCTAAATCGCac > 7:257821/1‑134 (MQ=255) cTGCTCGGAATCATCATCGGAGTCGCGGCGGTTGTGGTGATTTTGGGTGTGGGTGAAGGCAGCAAACACCCCGTAATGGCACAGATGGCCCCCGTCGGCGCCAACATACGAAACCGCCAATGCCGCCCCCCCAcc > 7:151181/1‑135 (MQ=255) cTGCTCGGAATCATCATCGGAGTCGCGGCGGGGGGGGTGATGTTGGCGGTGGGGTGAGGCAGCAAACGCCCGGTTATGGGACAGATTGCCCCCTTACGCGCCAACAACCAAAAACTAAATGGCCGCCCCCCCAccc > 4:256179/1‑136 (MQ=255) tCGGAATCATCATCGGAGTCGCGTCGGGGGTGGTGAGGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGC‑‑CCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTgc < 2:237527/133‑1 (MQ=255) cGGAATCATCATCGGAGTCGCGTCGGTGGGGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGCCCCTgcg > 8:63656/1‑135 (MQ=255) tcatcatcGGAGTCGCGTCGGTGGTGGTGATGTTGGCGGGAGGGGAAGGCAGCAAACGCCAGGTAAATGCACAGATTGCCGCCTTCGGCTCCACCATACAATAACTCAATGGCCGACCCCCCACCCAgcccccac > 7:245585/1‑129 (MQ=255) tcatcGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGc < 4:230944/136‑1 (MQ=255) tggcgcgTCGGGGGTGGGGATGTTGGCTGTCGGGGACGGCAGCAAACGCCAGGTAATCGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgcg < 1:275450/132‑1 (MQ=255) gggtgtggtgAGGTGGGCGGGGGGTGAAGGCAGAAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCGCATCACCCt < 1:163746/131‑1 (MQ=255) aTGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCGCATCACCCTGGACGATGtt < 5:248272/135‑1 (MQ=255) | CTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCGCATCACCCTGGACGATGTT > KT2440/4749180‑4749432 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |