| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,927,692 | A→C | 50.8% | W194G (TGG→GGG) | srp54 ← | Signal recognition particle 54 kDa protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,927,692 | 0 | A | C | 50.8% | 0.2 / 10.6 | 23 | W194G (TGG→GGG) | srp54 | Signal recognition particle 54 kDa protein |
| Reads supporting (aligned to +/- strand): ref base A (8/2); new base C (7/5); total (15/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01 | |||||||||||
AGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGATTCC > KT2440/4927565‑4927823 | aGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCAGGCCTgg > 2:276238/1‑135 (MQ=255) aGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCCGGCCTGGCGCGGcgcgt > 4:208693/1‑132 (MQ=255) ggtggtCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGCTCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCCGGCCAGGGGCCGCGCGTCgcg > 1:316732/1‑133 (MQ=255) gggtCTTCCCCCGGCCCGCCGGCCCGACCATGCCGAACCCCCCCCCCTCTGCGCTCGGCGCTATTCCCGGCTCTTCTAACACCCGCCCAAAGTCAGCCA‑GCACCAGGCGCCCGCCCGGGCGCGGCTCTTCGAtct < 2:168537/134‑1 (MQ=255) tCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACGTCGCTCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCCCGCCTGGCGCGGCGCTTCGGGCTcggc > 3:318471/1‑136 (MQ=255) cggccccATGCCCGCCGGGCCGACCATGCCGATCCCCCCCCCCGATCCCCGGGCCGGGATTCCCGGCACTGCGCTCA‑CCCTGCCAAAGGCACCACGCGGCAGCCGCCTGGCGGGCCGCGGCTCTTCGATCTcggcg < 4:17282/132‑1 (MQ=255) cccATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCAGGCCGGGCGCGGCGGGTCGCGCTcggcggg > 3:97350/1‑133 (MQ=255) cATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCGTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCAGGCCAGGCCGGGCGCGACCATCTcggcggggagg > 4:72560/1‑131 (MQ=255) gCCCGCCGGGCCGACCCTGGCGACCCCCCCACCTGCTGCGAGCGCCGCGATTCCTGCCACTTCCAACACCCGTCCCAAGGGCGCCC‑GCCGCAGCCGCCAGGCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTc < 6:122328/135‑1 (MQ=255) cgcctGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCCGGCCTGGCGCGGCTCTTCGGTCTCGGCGGGCAGGTcccg > 6:265649/1‑133 (MQ=255) cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCCGGCCGGGCGCGGCGCGTGGGTCGCGGCGGGCCGGTcccg > 8:99948/1‑133 (MQ=255) ggCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCAGGCCGGGCGCGGCGCTTCGCTCTCGGGGGTGCGGTCCAGCCGCTc > 4:364457/1‑136 (MQ=255) gCGAACCCCCCACCCTCTCCGAGCGGCGCGATTCCCGCCACTACGAACCCCGGGCCCACGGGCCCCA‑CCCCCAGCCGCCCGCCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATc < 2:281179/135‑1 (MQ=255) cccacccTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCCGGCCAGGCGCGGCTCATCGATCTCGGCGGGGAGGTCCACCCGCTCGCGGGCGCTCGGCCCGGa > 6:196701/1‑135 (MQ=255) ggCTCGATTGCAGGCACTTCGATCATCCGGGCCACGGGCGCCA‑GCAGCATGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCa < 4:44761/136‑1 (MQ=255) ggCGCGATTGCCGGCCCTCCGAACATCCGTCCCACGTGCCCCC‑GCCGCAGGCGCCCGCCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCa < 4:236683/136‑1 (MQ=255) gATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCCGGCCTGGCGCGGCTCGTCGCTCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAggcgg > 4:248502/1‑136 (MQ=255) aCTTCGATCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCAGGCCTGGCGCGGCGCGTCGATCTCGGCGGTGAGGTCCAGCCGCTCGCGGGCGATCGGCCCCGCCCGGCCGAAGCGCTGCACGCGGCGCCacacgt > 3:102988/1‑131 (MQ=255) agccGGGCCAAGTGCGCCA‑CCCGCAGGCGCCAGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCctgct < 4:85000/134‑1 (MQ=255) ctgcccgCCA‑GCAGGAGCCGCCCGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTtg < 8:17288/129‑1 (MQ=255) gCCA‑GCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCCGGGCCAGCAGCTCGCGGGCGATCGGCCCGGCCAGGCCGCTGCGCTGCAGGCGGCGCCAAAGGTTTGCCTGCTGCGGCTTGCTGCCCt > 4:234801/1‑136 (MQ=255) gcagcaTGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCCGCCGCTAGCGGGCGATCGGCCCGGGCAGGCCGATGCGCTGCAGGCGGGGCCCAAGGTTGGCCGGCTGCGGGTTGCGGGCCGGCAg > 4:296442/1‑136 (MQ=255) gcCAGGCCTGGCGCGGCTCTTGGATCTCGGCGGTCAGGTGCAGCAGCTCGCGGGCGATAGGCCCGGGCAGGCCGAGGCGCTGCCGGCGGCGCCCAAGGTTTGCCTGCTGCGGCTTGCTGCCCTGCCGCTGATTcc > 2:46617/1‑135 (MQ=255) | AGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCA‑GCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGATTCC > KT2440/4927565‑4927823 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |