Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,959,063 G→A 100% Q40* (CAG→TAG)  norR_4 ← Anaerobic nitric oxide reductase transcriptionregulator NorR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,959,0630GA95.7% 235.0 / 1.0 69Q40* (CAG→TAG) norR_4Anaerobic nitric oxide reductase transcriptionregulator NorR
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (46/20);  total (48/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGCAATAGCAACTTC  >  KT2440/4958938‑4959194
                                                                                                                             |                                                                                                                                   
cGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCAtg                                                                                                                            >  6:270120/1‑135 (MQ=255)
       aaCTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGca                                                                                                                     <  6:176010/135‑1 (MQ=255)
                   gTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGGGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGtttt                                                                                                        <  3:114717/136‑1 (MQ=255)
                    tAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGCGCATTCACGGTGCCGATGAGCACGCACCGCACTTCGCGGCTCGAGGACAAAGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGtttt                                                                                                        >  1:168581/1‑135 (MQ=255)
                    tAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTttc                                                                                                       >  4:275105/1‑136 (MQ=255)
                         aCTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGc                                                                                                  >  3:361462/1‑136 (MQ=255)
                         aCTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACATCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCg                                                                                                   >  8:252141/1‑135 (MQ=255)
                          cTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGc                                                                                                  >  5:270774/1‑135 (MQ=255)
                              cTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGa                                                                                               >  2:171611/1‑134 (MQ=255)
                              cTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGa                                                                                               >  5:11735/1‑134 (MQ=255)
                                    cagcCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGtt                                                                                        >  3:308340/1‑135 (MQ=255)
                                       cccAAGCCGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAg                                                                                     <  5:222054/135‑1 (MQ=255)
                                       cccAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAg                                                                                     <  5:193571/135‑1 (MQ=255)
                                          aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                                  >  5:35874/1‑135 (MQ=255)
                                          aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                                  >  5:218285/1‑135 (MQ=255)
                                          aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                                  >  2:243443/1‑135 (MQ=255)
                                          aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                                  >  4:364452/1‑135 (MQ=255)
                                          aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                                  >  6:13470/1‑135 (MQ=255)
                                          aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                                  >  7:368717/1‑135 (MQ=255)
                                          aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGca                                                                                   >  5:370101/1‑134 (MQ=255)
                                          aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGca                                                                                   >  6:58113/1‑134 (MQ=255)
                                          aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGca                                                                                   >  8:7357/1‑134 (MQ=255)
                                          aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGACTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                                  >  6:354967/1‑135 (MQ=255)
                                            gCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcaccac                                                                               <  4:61549/136‑1 (MQ=255)
                                             cAGAGTGCCCGGCCCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCGCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcaccac                                                                               <  2:83700/135‑1 (MQ=255)
                                                    cccGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAgg                                                                        >  1:128804/1‑135 (MQ=255)
                                                    cccGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAgg                                                                        >  5:181582/1‑135 (MQ=255)
                                                    cccGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGt                                                                       >  4:60345/1‑136 (MQ=255)
                                                    cccGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGt                                                                       >  4:283429/1‑136 (MQ=255)
                                                             ttCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCgcggcggc                                                              >  4:201588/1‑136 (MQ=255)
                                                             ttCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCgcggcgg                                                               >  7:249498/1‑135 (MQ=255)
                                                                aCGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGgcgc                                                            <  3:283429/135‑1 (MQ=255)
                                                                 cGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGgcgc                                                            <  6:218285/134‑1 (MQ=255)
                                                                 cGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGgcgc                                                            <  2:70753/134‑1 (MQ=255)
                                                                        gATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTgtc                                                    >  7:357354/1‑135 (MQ=255)
                                                                         aTGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTgtcg                                                   >  6:61987/1‑135 (MQ=255)
                                                                         aTGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTgtcgt                                                  >  4:73785/1‑136 (MQ=255)
                                                                         aTGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTgtcg                                                   >  2:3975/1‑135 (MQ=255)
                                                                         aTGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTgtcg                                                   >  5:23444/1‑135 (MQ=255)
                                                                                 gcacAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGAt                                           >  5:273035/1‑135 (MQ=255)
                                                                                 gcacAGCACTTCGCGGCTCGAGGACAATGGATCGACCGCCTGCTACCAGTCATCGCTGGCACACGCGAGGGTTTCTTCGCCGAGGATGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCGGTCGTCATCGa                                            >  5:210431/1‑134 (MQ=255)
                                                                                   acaGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATcag                                        >  4:195465/1‑136 (MQ=255)
                                                                                   acaGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATca                                         >  1:252472/1‑135 (MQ=255)
                                                                                   acaGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATca                                         <  2:16790/135‑1 (MQ=255)
                                                                                               ggCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTgg                             >  1:318387/1‑135 (MQ=255)
                                                                                               ggCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTgg                             >  1:244589/1‑135 (MQ=255)
                                                                                               ggCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTgg                             >  5:260979/1‑135 (MQ=255)
                                                                                               ggCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGt                            >  3:144594/1‑136 (MQ=255)
                                                                                               ggCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGGCGTCATCGATCAGCAGCATCTTGGt                            >  3:242087/1‑136 (MQ=255)
                                                                                                 gtCGAGGACAATGGCGCGACCGCCTGCTACCAGTCATGGCGGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGtt                           <  2:168581/134‑1 (MQ=255)
                                                                                                 cTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGtt                           <  6:370101/135‑1 (MQ=255)
                                                                                                    gAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCAc                       >  4:291492/1‑136 (MQ=255)
                                                                                                    gAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCAc                       >  4:287179/1‑136 (MQ=255)
                                                                                                    gAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCAc                       >  4:242263/1‑136 (MQ=255)
                                                                                                    gAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGGCGTCGGCGGTCCGCAGAATCTTGGTGTCa                        >  5:304292/1‑135 (MQ=255)
                                                                                                         cAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCca                   <  6:270774/135‑1 (MQ=255)
                                                                                                          aaTGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCcac                  <  2:326229/135‑1 (MQ=255)
                                                                                                          aaTGGCGCGACCGCCTGCTACCAGGCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCcac                  <  6:348187/135‑1 (MQ=255)
                                                                                                             ggCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGc              <  3:195465/136‑1 (MQ=255)
                                                                                                              gCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATg               >  2:295371/1‑134 (MQ=255)
                                                                                                              gCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGc              >  4:21591/1‑135 (MQ=255)
                                                                                                               cTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCGTGGTTTCACGCcaccttca             >  2:155980/1‑130 (MQ=255)
                                                                                                                  gACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGCATCTTGGTTTCACGCCACATGCAATa          >  2:142663/1‑135 (MQ=255)
                                                                                                                  gACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCAc                       <  2:220495/122‑1 (MQ=255)
                                                                                                                  gACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCAc                       >  1:220495/1‑122 (MQ=255)
                                                                                                                   aCCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGCAATAg         <  2:131599/135‑1 (MQ=255)
                                                                                                                       ccTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGCAATAGCAACt    <  4:318145/136‑1 (MQ=255)
                                                                                                                       ccTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGCAATAGCAACt    <  3:73785/136‑1 (MQ=255)
                                                                                                                       ccTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGCAATAGCAACt    <  3:21591/136‑1 (MQ=255)
                                                                                                                        ctgctaCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCGGTCGTCATCGATCAGCAGAAGCTTGGTTTCACGCCACATGCTATAGCAACt    <  6:181582/135‑1 (MQ=255)
                                                                                                                          gctacCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGGCGTCATCGATCAGCAGAATCTTGGTTTCACGCCCCATGCAATAGCACCTTc  >  1:201714/1‑135 (MQ=255)
                                                                                                                             |                                                                                                                                   
CGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGCAATAGCAACTTC  >  KT2440/4958938‑4959194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑