| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,096,964 | G→C | 36.8% | intergenic (+70/‑120) | CJ019_04792 → / → CJ019_04793 | Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,096,964 | 0 | G | C | 36.8% | 28.2 / 8.2 | 29 | intergenic (+70/‑120) | CJ019_04792/CJ019_04793 | Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (15/2); new base C (5/6); total (20/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCACACTTGCGAGGAACGACGAGATGCATGAAATCC > KT2440/5096834‑5097096 | cGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTAATGGGGCATATGTGCGCTGTTGGCCCGCAAAACCGACaa > 1:115393/1‑135 (MQ=255) tcatGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCGTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGCCCggg > 3:108865/1‑136 (MQ=255) gcacgcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTGATGGGGGGTATTTGGGCGGTTAGCCCGCAAAAACCCCAAACGCCCGGGaaaaaaa > 5:302204/1‑135 (MQ=255) gcacgcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCGTTTTTCATGGGGGATATTTGCGGTGTTCGCCCGCCACACCCCCAAACCCCCCGGcaaaaaa > 2:123876/1‑135 (MQ=255) gcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTGATGGGGCATATTTTCGGTGGTAGCCCGCCAAAACCACAAAACACCCGGGAAAAAACCaa > 3:5420/1‑135 (MQ=255) gACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTg > 7:94312/1‑134 (MQ=255) ggggCGTAATCGGCTTATGGCGGTATTTAACCACCTGATGGATGGGGCCAGAGGCCCTTTTTTTCATGGGGAATTTGGGCGCTGTGCGCCCGCAAACCCTACAAACGACCGGGAAAAAAACCAAACTGTCATcc < 7:245436/132‑1 (MQ=255) ggCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGCGGCCCGTGTTTTGATGGGGCATATGTTCGGTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCAACCAg > 6:179914/1‑134 (MQ=255) gAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGGTTTTGATGGGGGATATTTGGGCTGTTCGCCCGCAAAAACGACAAACGACCGCGAAAAAAACCAAACTGTCATCCaacatag > 2:13558/1‑129 (MQ=255) aaGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTTTCCTGCCCGAGCtt > 7:56647/1‑135 (MQ=255) aaggaaggGGCCAGAGGCCCGTTTTTTAATGGGGGGGAATTGCGCTGGTGGCCCCCAAAACCCACCAACACCCCCGAAAAAAACCAAACTGTCATCCAGACATGTGTATCAGGGCCCACCTGTTTGAAGcgagaa > 6:279729/1‑130 (MQ=255) ccAGAGGCCCGGTTTTTGATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGCCCAACGCCCGGGAAAAAAACCAAAAAGTCATCCCGACGTGTGTATGCTGCCCGCGCGGTTTGAAGCTACAAACGCGCCTgg > 2:84115/1‑135 (MQ=255) ttttttCATGGGGCATATGTTCGCAGTTAGCCCGCAAAACCCACAAACGCCCCGGAAAAAAACCAAACTGTCATCCACACATGTGTATACAGCCCGAGCGTTTTGAAACAACCAACCCGCCTGGCGCACACttt > 8:50976/1‑134 (MQ=255) ttttttCAGGGGGCATATGGGCGCTGTGCCCCCGAAAACCCCACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTtc < 4:108865/136‑1 (MQ=255) gtttttCATGGGGCATATGTGCGCGGGTAGCCCGCAAAACCGACAAACGCCCGGGAAAAAAACCAAACAGTCATCCAGACGTGTGGATCCTGCCCGCGCTTTTTGAAGCTACACACGCGCCGGGCGCAGACgttt > 2:8591/2‑135 (MQ=255) gggCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCACACtt > 4:79201/1‑136 (MQ=255) cggTTCCCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCACACTTGCGAGGAacgac < 7:129732/133‑1 (MQ=255) cTGTGCGCCCCAAAAACCCACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCAGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCTCACTTGCGAGGAacgac < 1:60749/135‑1 (MQ=255) tGTTCGCCCGCAAAACCGACACACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAACCGCGCCTGGCGCAGACTGTTCACTCACACTTGCGAGGAccga > 7:75110/1‑133 (MQ=255) tGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAACCTGTCATCCAGACTTCTCTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTATTCACTCACACTTGCGAGGAacga > 5:30280/1‑133 (MQ=255) tGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCACACTTGCGAGGAacga > 7:87464/1‑133 (MQ=255) tGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTGTTCACTCACACTTGCGAGGAacga > 1:356214/1‑133 (MQ=255) ttCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTGTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCACACTTGCGAGGAacga > 1:56288/1‑131 (MQ=255) gcGCCCGCAAAACCCAAACACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCACACTTGCGAGGAACGACgaga < 6:115208/134‑1 (MQ=255) gCCCACAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCACACTTGCGAGGAACGACGAGAt > 8:110366/1‑134 (MQ=255) cccGAAAAACCCACACACCACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCACACTTGCGAGGAACGACGAGATGc < 8:85713/135‑1 (MQ=255) ctcaaaaaCACACAAACGACAGGAAAAAAAACCAAACTGTCTTCAAGACTTGTGTATCCTGCCCGAGATTTTTGAAGCTACAAACGCGGCTGGCGCAGACTTTTCACTCACACTTGCGAGGAACGACGAGATGCa < 1:169651/131‑1 (MQ=255) aaaCCGAACAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTTCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCACACTTGCGAGGAACGACGAGATGCATGaaa < 7:63132/135‑1 (MQ=255) aCCCACACACCACCGGTAAAAAAACCCAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCACACTTGCGAGGAACGACGAGATGCATGAAATcc < 4:5420/136‑1 (MQ=255) | CGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCACACTTGCGAGGAACGACGAGATGCATGAAATCC > KT2440/5096834‑5097096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |