Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,358,311 T→G 23.8% H50P (CAC→CCC)  folP ← Dihydropteroate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,358,3110TG23.8% 65.4 / 3.2 42H50P (CAC→CCC) folPDihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base T (13/18);  new base G (4/7);  total (17/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGG  >  KT2440/5358177‑5358441
                                                                                                                                      |                                                                                                                                  
gACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTg                                                                                                                                   <  4:290574/136‑1 (MQ=255)
     ttGATGGCCTCGCCCATTGGCGCCACACGCTCCAGCTCCTCGGTCCCCGAGACTGCACGCGCACCCGGGCGCGGGGACACGGCCCCGAGATGGCTCAACCTTGCGCCGGCGGGACCCAACCCGTCCGCGGGgcgg                                                                                                                               >  1:229557/1‑134 (MQ=255)
               cgcccATGGGCGCCACCCCCTCCTGCTCCTCGGCCACCGAGACTGCCCGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCtcgtc                                                                                                                    <  3:70290/133‑1 (MQ=255)
               cGACCATTGCCACCCCACGCCCCAGCTCCTCGGTCACCGCGACTGCCCGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCtcgtc                                                                                                                    <  3:172309/136‑1 (MQ=255)
                  ccATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGTCTCGCCCCCCCAATAGAACAAACTTTCGCCCGCGGCAACCAAAGCTTCGGCGGGGCGCAGGGGCTCCTcgcg                                                                                                                 >  4:51861/1‑136 (MQ=255)
                  ccATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACACGGGCGCGTGGGCTCGCCCCCCCTAACGCTACACGTTGCGCCGGCGGGCACCAACGCTTCGGCGTGGCGCCGGGCCtcggggc                                                                                                                  >  6:56503/1‑131 (MQ=255)
                                      aGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGGGGGCTCGCCCCCGATATAGATAAAACTTTCGCCCGCGGCCACCAAAGCTTAGGGCTGGCGCCGGGCCGCGGAGCGCCGGCAGAAACGCCCGCCa                                                                                              >  8:159544/1‑135 (MQ=255)
                                         tcctcGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCg                                                                                           <  8:101980/135‑1 (MQ=255)
                                           ctcGGACCCCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGAACAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGga                                                                                         <  8:86268/135‑1 (MQ=255)
                                                tCACCGAGACTGCACGCGCACCCGGGCGCGGGGGCTCGCCCCCCCGATCGGTCAACCTTTGGCCGGCGGCCACCATCGCGTCGGCGGGGGGCCGGGGCTCGGAGCGCGGCCGGCAGCGCCCCCCAGCGGGGAAgg                                                                                    >  4:320781/1‑135 (MQ=255)
                                                tCACCGAGACTGCACGCGCACCCGGGCGCGGGGACTCGCCCCCGATATCGATCAACCTTGCGCCCGCGGCCCCCATCGCTTCGGCGGGGCGCCGGGCCTCGTCGCGCGGGCTGCAGCGCCCGCCCTCGGAGAAgg                                                                                    >  4:103722/1‑135 (MQ=255)
                                                     gcgaCTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCgg                                                                              <  3:121505/134‑1 (MQ=255)
                                                      agaCTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCgg                                                                              <  6:166943/135‑1 (MQ=255)
                                                             aCGCGCACCCGGGCGCGTGGGCTCGCCACCGATATCGATCAACGTTGCGCCGGCGGGCACCATCGCTTAGGCGTGGCGCCGGGCCTCGTCGCGGGGGCTGAAGCGCCCGGCATCGGAGACGGGATGCGGGGTggt                                                                       >  8:272351/1‑133 (MQ=255)
                                                                  ccccggggCGGGTGGACTCGCCCCCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGGGGCGCAGGGCCTCGTCGCGCTGACTGACGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTga                                                                  <  2:75856/130‑1 (MQ=255)
                                                                    cccGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTgaga                                                                <  8:191144/135‑1 (MQ=255)
                                                                     cggggCGCGTGGACTCGCCACCGATAGCGATCACCGTTGCGCCGGCGGAAACCAGCGCTTCGGCGTGGCGCAGGGCCTCGGCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAAt                                                              <  3:37237/134‑1 (MQ=255)
                                                                      cGGGGGCGTGGACTCGCCACCGATATCGATCAACGTGGCGCCGGCGGCAACCATCGCTTCGGCTTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAAt                                                              <  7:159544/135‑1 (MQ=255)
                                                                             ggggACTCGCCACCGATATCGATCAACGGGGCGCCGGCGGCAACCATCGCTTGGGCGTGGCGCAGGGCCGCGGCGCGCTGACTCACGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATg                                                       <  1:136564/133‑1 (MQ=255)
                                                                              tGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGa                                                      <  6:124139/135‑1 (MQ=255)
                                                                                      ccACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTAt                                             >  3:161948/1‑136 (MQ=255)
                                                                                        aCCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATg                                            <  2:48566/135‑1 (MQ=255)
                                                                                         ccGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCACAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGc                                           <  8:77331/135‑1 (MQ=255)
                                                                                          ctatagCGATCACCGTTGCGCCGGCGGCAACCATCGCTGCGCCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCg                                          <  8:320089/133‑1 (MQ=255)
                                                                                                    cAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATaaa                                 >  1:142083/1‑134 (MQ=255)
                                                                                                    cAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAg                                >  4:6852/1‑135 (MQ=255)
                                                                                                       cGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAAc                             >  5:146908/1‑135 (MQ=255)
                                                                                                             gcCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTg                      >  4:6834/1‑136 (MQ=255)
                                                                                                               cggcggCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGc                     >  5:27904/1‑135 (MQ=255)
                                                                                                               cggcggCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGc                     >  7:181069/1‑135 (MQ=255)
                                                                                                               cggcggCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGc                     >  7:279677/1‑135 (MQ=255)
                                                                                                                 gcggcAACCATCGCTTCGGCGTGGCGCAGGGCCTCGGCGCGCTGACTGACGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCg                   <  5:169944/135‑1 (MQ=255)
                                                                                                                    gcAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCaa                >  2:204225/1‑135 (MQ=255)
                                                                                                                    gcAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCaa                >  8:89336/1‑135 (MQ=255)
                                                                                                                     caacaaGCGCTGCGGCGGGGCGCAGGGCCGCGGCGCGCTGACTGAAGCTACCGCCATCGGAGAAGGAAGCGGGGGTGATATTGATAATACCCTTGACATGTGTATGCGAAAAATCAAGAACCCGGCTGCCGAAAgg              <  4:316510/136‑1 (MQ=255)
                                                                                                                      aaCCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGCGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAgg              <  8:127674/135‑1 (MQ=255)
                                                                                                                      aaCCATCGCTTCGGCGGGGCGCAGGGCCGCGTCGCGCTGACTGACGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAgg              <  1:245143/135‑1 (MQ=255)
                                                                                                                        ccATCGCTTCGGCGGGGGGCTGGGCCGGGTCGCGCTGACGCCAGCGACCGCCATCGGAGAAGGAAGCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCa            <  2:338108/135‑1 (MQ=255)
                                                                                                                             gcgtCGGCGGGGCGCAGGGCCGCGCCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAAGCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgg       <  1:359360/132‑1 (MQ=255)
                                                                                                                             gCTGCGGCGGGGCGCAGGGCCGCGGCGCGCTGACTGACGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACCTGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgg       <  2:213904/135‑1 (MQ=255)
                                                                                                                                  ggCGTGGCGCTGGGCCTCGTCGCGCTGACGGCCCCGACCGCCATCGGAGAATGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATAAAGAACCCGGCTGCCGCAAGGCAACCgggtagg  <  6:259788/135‑1 (MQ=255)
                                                                                                                                  ggCGGGGGGCCGGGCCGCGGCGCGCTGACTCAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggtagg  <  7:210320/135‑1 (MQ=255)
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GACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGG  >  KT2440/5358177‑5358441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑