Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,546,108 T→G 20.4% V504G (GTG→GGG)  CJ019_05222 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,546,1080TG20.4% 95.5 / 2.0 51V504G (GTG→GGG) CJ019_05222hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (7/32);  new base G (6/6);  total (13/38)
Fisher's exact test for biased strand distribution p-value = 5.30e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAAAGAGCTGACGGCTACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCTTGCCCAGGCCGCCATGTGGCCTGGGCACAAGCCTGAGTGCCTAGACTTCATCACAAGTTCACTTTAAAACTCTCA  >  KT2440/5545979‑5546243
                                                                                                                                 |                                                                                                                                       
tGAAAGAGCTGACGGCTACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCgg                                                                                                                                    >  8:161114/1‑135 (MQ=255)
   atgagCTGACGGCTACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGGCACCGAGGTGTGGGCACAGACAGCCAACGTCGAGTTCGCGGCGGCGGGGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGtt                                                                                                                                <  3:219303/134‑1 (MQ=255)
    agagCTGACGGCTACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGtt                                                                                                                                <  1:115736/135‑1 (MQ=255)
    agagCTGACGGCTACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGtt                                                                                                                                <  1:105521/135‑1 (MQ=255)
    agagCTGACGGCTACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGtt                                                                                                                                <  6:91716/135‑1 (MQ=255)
     gagCTGACGGCTACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTg                                                                                                                               <  1:333229/135‑1 (MQ=255)
        cTGACGGCTACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCg                                                                                                                            <  1:317768/135‑1 (MQ=255)
         tGACGGCTACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGAGTGTGGGGGCGGGGTTGcccgg                                                                                                                          >  4:262315/1‑133 (MQ=255)
               cTACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTAttt                                                                                                                    <  3:130237/136‑1 (MQ=255)
                tACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTAttt                                                                                                                    <  1:4082/135‑1 (MQ=255)
                tACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGGGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTAttt                                                                                                                    <  7:56579/135‑1 (MQ=255)
                     tgctgcTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGGTGTTGATTGTGGTGTCGGGGGTGCCGGTGGATTTGCTGAc                                                                                                              >  4:264731/1‑136 (MQ=255)
                           tCAGCCCGACCGGCATGACCACCCTGGCGACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCTGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTAcgc                                                                                                         <  5:225182/135‑1 (MQ=255)
                           tCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTAcgc                                                                                                         <  2:160746/135‑1 (MQ=255)
                               cccGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTAc                                                                                                           >  7:133310/1‑129 (MQ=255)
                               cccGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTAc                                                                                                           <  8:133310/129‑1 (MQ=255)
                                ccGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGGTGGTGAATGTGGGGTCGGGGGGGCCGGGGTATTTGCGGCCTACGGGGATg                                                                                                    >  7:48610/1‑135 (MQ=255)
                                  gACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTGGTTGATTGTGGTGTCGGGGGTGCCGGGGGGTTTGGGGGCTCCGCGGgtgtg                                                                                                  >  7:348141/1‑134 (MQ=255)
                                  gACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCCCCGGATGGCGCGTTGGTGTTTTTTGGGTGGGGGTTGGCCGGGTTTTTTGTGGCAACGCGGgtgtt                                                                                                  >  7:359940/1‑134 (MQ=255)
                                    ccGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTAttt                                                                                               <  3:223476/136‑1 (MQ=255)
                                     cGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTAttt                                                                                               <  7:108980/135‑1 (MQ=255)
                                      ggCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGa                                                                                             <  4:217182/136‑1 (MQ=255)
                                       gCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGa                                                                                             <  6:98348/135‑1 (MQ=255)
                                       gCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGa                                                                                             <  7:236609/135‑1 (MQ=255)
                                       gCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGa                                                                                             <  7:315844/135‑1 (MQ=255)
                                       gCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGGATGCGGCGTTGTTGTATGTGGTGGGGGGGGTGGCGGTGTATTTTCTGACTACGCCGATGTAtttta                                                                                             >  4:305912/1‑133 (MQ=255)
                                        cATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGaa                                                                                            <  7:161114/135‑1 (MQ=255)
                                                  cTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGa                                                                                  <  6:105050/135‑1 (MQ=255)
                                                       cACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCtt                                                                            <  3:262315/136‑1 (MQ=255)
                                                         ccGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCTTg                                                                           <  1:74581/135‑1 (MQ=255)
                                                         ccGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCTTg                                                                           <  2:244521/135‑1 (MQ=255)
                                                          cGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGGATGCGGCGTTGGTGTTTTTGGGGGCGGGGGTGGCGGGGGTTTTTGGGTCTAAGCGGCTGTTTTTTTAAAAAGCGTGAAAGCTTTcc                                                                         >  3:299809/1‑136 (MQ=255)
                                                             ggTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCTTGCCCAg                                                                      <  3:305912/136‑1 (MQ=255)
                                                              gtgtGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCTTGCCCAg                                                                      <  8:38202/135‑1 (MQ=255)
                                                              gtgtGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCTTGCCCAg                                                                      <  1:207996/135‑1 (MQ=255)
                                                              gggtgGGCACACACAGCCAACGTCGAGTTCGCTGCTGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCTTGCCCAg                                                                      <  5:48399/133‑1 (MQ=255)
                                                                  gggCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCTTGCCCAGgccgc                                                                 <  3:295267/136‑1 (MQ=255)
                                                                  gggCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGGTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCAGCCTTCGCGGATGTATTTGAACAAGGCTTGAAAGCTTGCCCAGgccg                                                                  >  2:74581/1‑135 (MQ=255)
                                                                          acaGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGGGGTGTTTATTGTGGGGGCGGGGTTGGCGGTGGATTTTCTGCCGACGCGGGTGTATTTGAACAAGGCGTGAAAGCTTGCCCCGGCCGCCCTGTggg                                                         >  3:144628/1‑135 (MQ=255)
                                                                               caccGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTGGTTGATTGTGGTGGCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTTAACAAGGCTGCAAAGCTTGCCCAGGCCGCCATGTGGCCTgg                                                     <  8:124751/132‑1 (MQ=255)
                                                                                aaCGGCGAGTGCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGGGGTGGCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGACCAAGGCTTGAACGCTTGCCCCGGCCGCCATGGGGCCGGGGc                                                   <  3:340744/136‑1 (MQ=255)
                                                                                 aCGTCGAGTTCGCGGCGGCGGCGCCGGATGCGGCGTTGTTGATTGTGGGGTCGGGGGTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAATGCTTGAAAGCTTGCCCCGGCCGCCATGTGGCCTGGGc                                                   >  7:120775/1‑135 (MQ=255)
                                                                                 aCGTCGAGTTCGCGCCGGCGCCGCCTTAGGCGGCGGTGTTGCTGGTGGGGTCGGGGTTGCGGGTGTATTGGCTCACTACGCGGATGTATTTGAACATGGCTTGACCGCTTGCCCAGGCCGCCATGTGGCCTGGGc                                                   <  6:70920/135‑1 (MQ=255)
                                                                                       aGTGCGCGGCGGCGGCGCCGTATGCGGCGTGGTTGATTGTGGGGCGGGGGGGGCCGGTGTATTTGCTCCCTACGCGGAGGTATTTGAACACGGCTTGAAACCTGGCCCAGGCCGCCTTGGGGCCGGGGCACAAGcc                                            <  4:188139/136‑1 (MQ=255)
                                                                                        gtgcgcgGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCTGGCCCAGGCCGCCAGGTGGCCGGGGCACAAGcc                                            <  2:273408/132‑1 (MQ=255)
                                                                                        gtgcgcgCCGGCGGCCCCGTAGGCGGCGTTGTTGTTGGTGGGGGCGGGGTTGCCGGTTTATTTGGTCACTACGCGGATGTATTTGAACAAGGCTTGAACGCTTGCCCCGGCCGCCATGTGGCCTGGGCACAAGcc                                            <  8:271468/132‑1 (MQ=255)
                                                                                                                gtcgTTGTTGATTGTGGGGTCGGGGTGGCCGGTGTTTTTGCTGAATACGCGGATGTATTTGAACAAGGCTTGAAAGCTTGCCCAGGCCGCCATGTGGCCTGGGCACAAGCCTGAGTGCCTAGACTTCATCACaa                     <  5:186682/132‑1 (MQ=255)
                                                                                                                      gttgATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTAttt                                                                                               <  7:196956/54‑1 (MQ=255)
                                                                                                                      gttgATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTAttt                                                                                               >  8:196956/1‑54 (MQ=255)
                                                                                                                          tttGTGGGGTGGGGGTTGCGGGTTTTTTTGCTGACTACGGGGATGTATTTCAACTCTGCTTTAAAGCGTGCCCAGGCCGCCATGGGGCCTGGGCACAAGCCTGAGTGCCTAGACTTCATCACAAGTTCACTTTa           <  2:326550/133‑1 (MQ=255)
                                                                                                                          aTTGGGGGGGCGGGGTTGCGGGTGTATTTGCTGAGTACGCGGATTTATTTGAACACGGCTTGAAAGCTTCCCCAGGCCGCCAGGGGGCCGGGGCACAAGCCTGAGTGACTAGACTTCATCACAAGTTCACTTTaa          <  6:207201/135‑1 (MQ=255)
                                                                                                                                 tgtCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCTTGCCCAGGCCGCCATGTGGCCTGGGCACAAGCCTGAGTGCCTAGACTTCATCACAAGTTCACTTTAAAACtctc   >  1:260714/1‑135 (MQ=255)
                                                                                                                                 tgtCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCTTGCCCAGGCCGCCATGTGGCCTGGGCACAAGCCTGAGTGCCTAGACTTCATCACAAGTTCACTTTAAAACTCTCa  >  4:351150/1‑136 (MQ=255)
                                                                                                                                 |                                                                                                                                       
TGAAAGAGCTGACGGCTACCCTGCTGCTCAGCCCGACCGGCATGACCACCCTGGCCACCGAGGTGTGGGCACACACAGCCAACGTCGAGTTCGCGGCGGCGGCGCCGTATGCGGCGTTGTTGATTGTGGTGTCGGGGTTGCCGGTGTATTTGCTGACTACGCGGATGTATTTGAACAAGGCTTGAAAGCTTGCCCAGGCCGCCATGTGGCCTGGGCACAAGCCTGAGTGCCTAGACTTCATCACAAGTTCACTTTAAAACTCTCA  >  KT2440/5545979‑5546243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑