| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,689,790 | T→G | 49.0% | V227G (GTG→GGG) | pyrC' → | Dihydroorotase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,689,790 | 0 | T | G | 49.0% | 0.7 / 6.3 | 20 | V227G (GTG→GGG) | pyrC' | Dihydroorotase‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (6/4); new base G (3/7); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGACCGCGACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCG > KT2440/5689656‑5689919 | ggACCGCGACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCACCCTGCTGCTGGTGGACCAGAGCGgcgt > 8:169989/1‑135 (MQ=255) gACCGCGACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGgcgtgc < 3:8206/136‑1 (MQ=255) aCCGCGACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGgcgtgcg < 3:135312/136‑1 (MQ=255) aCCGCGACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGgcgtgc > 2:10117/1‑135 (MQ=255) ccTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGgcgcc > 6:139096/1‑134 (MQ=255) cccAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGACACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGgcgcgcctga > 2:330777/1‑129 (MQ=255) ggTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGCGCGCGTGCCCCACATCACCAcc‑cccccggtc > 1:243710/1‑130 (MQ=255) tGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCGTCAGCCAGCTCACCAGC‑GCCCGTGGCGCGCGGc > 8:258586/1‑135 (MQ=255) aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGTGGGCACTTAAGCCACATAACCACC‑GCCCCTTGCGCCCGGCGGATCg > 4:329494/1‑136 (MQ=255) ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGGGGGGCAGTCAGCCACAAAACACGC‑CCCCGTGTCGCGCCGCGGAAAgccca > 6:283454/1‑135 (MQ=255) gggggCCGGCGGGGAACCCCGCAGCGCCCCCAAACGGGGGCCGGGGCGCGCAACCGGGTGGGGGGGGACCCGCGGGGGGGGCGGGCGCAGTACCGCCAGAACCCCCCC‑GCCGGTGGCGCGCGGCTGATCgcccag < 6:362330/132‑1 (MQ=255) ccTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGC‑GCCCGTGGCGCGCGGGGGGTCgcccaggcccc > 7:74020/1‑134 (MQ=255) ggCAGCCCGGAGCGCGCCGAAAGCGGGGCCCGGGCGCGCAACCGGCGGCGGGTGCAACAGCGCGGGGGGCGGGCGCACTTCCCCCAGAACACCCGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg < 7:169989/135‑1 (MQ=255) ccGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGCGCACGTCAGCCAGATCCCCACC‑GCCCGTGGCGCGCGGCTGCTCGCCCCGGCCCCCGCCGTGGGCCTg > 2:68987/1‑135 (MQ=255) ccGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGGGGGGGCCCTTCAGCCCGATCACCCCC‑GCCCCGGGCGCGCGGGGGCTCGCCCCCGCCCCCGCCCTggggcgg > 1:111976/1‑131 (MQ=255) ggAGCGCGCCGAAACCGGGGCCCTGGCTCGCACCCTGCTGCGGGGGGAACAGCGCGGCGTGCGTGCGAACTTCAGCCAGATCACCCGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGcc < 6:80774/135‑1 (MQ=255) gcgcCGAACCGGGGGCCCTGGCGCGCAACCTGCTGCTGGGGCACCAGAGCGGGGGGCGTGCGCACTTCAGCCAGATCACCCGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 3:30707/136‑1 (MQ=255) gcgcCGAAACCGGGGCCCTGGCGCGCAACCTGCGGGGGGTGCAACAGTGCGGGGGGCGGGCGCACTGCACCCATATCACCCGC‑GCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 3:26036/136‑1 (MQ=255) cgcCGAAACGGGGGCCCTGGCGCGAAACCTGGGGCGGGGGCAACAGCGCGGCGGGCGTGCGCACTGCCCCCCCACCCCCCGC‑GCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 1:228882/135‑1 (MQ=255) cgcCGAAACCGTGGCCGTGGCGCGCAACCTGCTGCTGGTGCAACAGAGCGGGGGGCGGGCGCACTTCAGCCAGATCACCCGC‑GCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 5:80661/135‑1 (MQ=255) gaaccggTGGCCCGGGCGCGCAACCTGGTGCGGGGGCAACAGCGCGGCGTGCGTGCGCACTGCCCCCACAGCCCCCCC‑GCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg < 4:15362/130‑1 (MQ=255) gTGGGCCGGGCGCGCAACGGGGGGGGGGGGGAACCGGGGGGGGGGGGTGGGGACTTCAGCCACCTCCCCCGCGGCCGGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 4:281025/136‑1 (MQ=255) gTGGCCCTGGCGCGAACCCGGCGGCTGGTGGAACTGCGCGGGGTGCGTGCGCACTTCCGCCAGATCCCCCCC‑GCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 8:22193/135‑1 (MQ=255) cggcgTGCGTGCGCACTTCACCCAGATCACCAGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 8:74020/135‑1 (MQ=255) | GGACCGCGACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCG > KT2440/5689656‑5689919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |