| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,094,831 | 0 | T | G | 69.6% | ‑3.5 / 10.7 | 26 | K178N (AAA→AAC) | CJ019_01995 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (16/0); total (22/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.16e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAAGCCCTCATGGGCAGCGCGGGTCA > KT2440/2094707‑2094961 | cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCGGGGCGTTTTGGAggg > 1:249473/1‑135 (MQ=255)cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCGGGGCGTTTTGCAggg > 4:354325/1‑135 (MQ=255)cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCCGGCGGGGCGGGGCGGTTTGCgggg > 5:203233/1‑135 (MQ=255) gCCCGGGCCAACAGTTTCTCAAGATACGTCATCTTCTCTTCAAACACCTTCGGCTCCGGCAGCTTGCGCAAATACTGCACGCCGCGCTCGAACGCCCTGCCCGCCAGGCCGGGCCTGCCCTTTTGCAagggcggt > 6:291884/1‑130 (MQ=255) gCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCGCCAGGCCGGGCTGGCCGTTTTTCAGGGCCAGt > 2:257340/1‑135 (MQ=255) cAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGGCCCGGCTGGCCGTTTTTCACGGCCAGGTGGCCGAg > 6:167110/1‑135 (MQ=255) cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCGGGTGGCCGAGGGTGTTgaag > 2:6548/1‑134 (MQ=255) cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGGCCGGGCGGGCCGTTTTTGAGGGCCGGGTGGCCGAGGTTGTTgaag > 5:142130/1‑134 (MQ=255) acacCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGGCCGGGCTGGCCGGTTTGCAGGGCCGGGTGGGCGTCGGTGGTGAAGAACTCTATATGCAGCAGCCCCAgca > 7:252092/1‑133 (MQ=255) ttCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCGGGCCGGGTTGCAGGGCCGGGTGGGCGAGGGTGTTGTAGCACTCTATATTCAGCCGCACCACGAGATgg > 3:362116/1‑135 (MQ=255) ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCGGGCCGTTTTGCAGGGCCTGTTGGGCGAGGTTGTTGAAGAACTCTATATTCAGCAGCCCCAGGATATGGc > 2:226949/1‑133 (MQ=255) ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCGTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCACGATATGGCgg > 8:167118/1‑135 (MQ=255) cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGGCCGGGCTGGCCTTTTTGGAGGGCCGGGTGGCCGCGGGTGTTGAAGCACTCTATATTCAGCAGCCCCCGGCTATGGCGGag > 8:12440/1‑134 (MQ=255) cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCGCCTGGCCGGGCTGGCGGTTTTGCACGGGCGGTTGGGCGCTGGTGTTGTAGACCTCTATAATCAGCAGCACCAGGAGATGGCggct > 3:59691/1‑133 (MQ=255) tgctTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGGCGTTTTGCAGGGGCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATTGCGGATTTCCTTg > 4:89352/1‑135 (MQ=255) tgctTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCGGGCCGTTTTGCCGGGCCTGTTGGCCGCGGGTGTTGAAGAACGCTATATTCCGCCGCCCCAGGTTATGGGGGCtttgcgtt > 4:321427/1‑130 (MQ=255) cAAATACTCCACGCCGCGCTCGACGCCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATg < 4:56388/135‑1 (MQ=255) aaattACGGCACGCCGCGCGCGATGGCCGGGCCGGCCTGGCCGGGCTGTCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGTCGTATTTCCTTGACCCaa < 7:289103/129‑1 (MQ=255) cgccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTTCCGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGCTTTCCTTGACCCAATGCTTGGCCACg > 8:5430/1‑134 (MQ=255) gccgcGCTCGAAGGCCCCGCGCGCCTGGCCGGGCTGGCCATTTTGCCGGGGCTGGTGGGCGAGGTGTTTGAAGAACTCGATATGCAGCAGCGCCCGGATATTGGGGATTTACTTGACCCCATGCGTGGCCTCgcc > 6:258317/1‑133 (MQ=255) gccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCATTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTACTTGACCCAATGCATGGCCTCGAc > 6:335032/1‑135 (MQ=255) cgcTCGACGCCCCGGCCCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCg < 6:142130/135‑1 (MQ=255) gcTCGAAGGCCCGGCGCGCCAGGCCGGGGTGGGCGTTTTTCCGGGGCGGTTGGGCGCGGGTGTTGAAGAACTCGATATTCAGCCGCACCAGGATATGGCGGGTTTCCTTGGCCCAATGCTTGGCCGCGACAGCgg > 4:2358/1‑135 (MQ=255) gcTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCGTTTTGCAGGGCCTGGTGGCCGAGGGTGTTGAAGCACTCTATATGCCGCCGCACCCGGCTATGGCGGATTTCCGTGACCCAATGCGTGGCCTCGACACCgg > 6:157637/1‑135 (MQ=255) ctacggCCAGCCGCGGCGGCCCGGGCTGTCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCa < 4:325851/131‑1 (MQ=255) ggCCGTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAACAAGCCCTCATGGGCAGCGCGGGTCa > 2:362987/1‑135 (MQ=255) | CATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAAGCCCTCATGGGCAGCGCGGGTCA > KT2440/2094707‑2094961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |