Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,770,0800AC42.1% 46.8 / 13.0 57V161G (GTG→GGG) CJ019_02613hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (11/22);  new base C (22/2);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 8.80e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTT  >  KT2440/2769947‑2770213
                                                                                                                                        |                                                                                                                                     
gCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCAc                                                                                                                                      <  1:31824/135‑1 (MQ=255)
    aGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCACCCGCCCCGGTGCCTGCAGCCACCCGG‑CG‑CGcccccccccc                                                                                                                                  >  2:178953/1‑135 (MQ=255)
     ggCCCGTTGTCGGGCAAGTAGAGGCTCAGGCGAATACT‑GCAGCCCATGCCGATATCAACAAAACCCGCGGCAACTGTTGGTTGAACACGCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCa                                                                                                                                 <  2:78326/135‑1 (MQ=255)
     ggCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACT‑GCAGCCCATGCCGATATCAACACACCCCCCGCCACCCCTTGGGTGAACACCCGCACCCGCCCCCGGGGCTCCCGCCCCCCGG‑CG‑CGccccccccccc                                                                                                                                 >  7:263707/1‑134 (MQ=255)
              tCGGTCAAGTAGAGGCTCAGGCCAATACT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGccc                                                                                                                        >  4:255578/1‑135 (MQ=255)
              tCGGTCAAGTAGAGGCTCAGGCCAATACT‑GCAGACCATGCCGATATCAACAAAACCCCCGCCAACACAAGGTTGAACACCCGCAACCGCCCCCGAGCCTGCAGCCACCGGG‑CG‑CTCCCCCCCCCCCAcccccccc                                                                                                                        >  1:199716/1‑135 (MQ=255)
               cGGTCAAGTAGAGGCTCAGGCCAATACT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACATGGTTGAACACCCGCAACCGCCCCGCTGCCTGCCGCCACCCGG‑CG‑CTCCCCCCCCCCAACAccccccc                                                                                                                       >  4:213957/1‑134 (MQ=255)
                 gTCAAGTAGAGGCTCAGGCCAATACT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAgg                                                                                                                     <  7:59069/135‑1 (MQ=255)
                 gTCAAGTAGAGGCTCAGGCCAATACT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAgg                                                                                                                     <  3:91723/135‑1 (MQ=255)
                        acagGCGCAGGCCAATACT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCAt                                                                                                              <  1:326086/133‑1 (MQ=255)
                          cgccccAGGCCAA‑ACTCGCACCCAAGGCCGATTTCAACAAAACCCCCGCCAACCCTTGGGAGAACACCCGCAACCGCCCCGGGGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCCACACCGCCCAGGCGGCCAt                                                                                                              <  1:128129/128‑1 (MQ=255)
                                   ccAATACT‑GCAGCCCTTGCCGATATCAACAAAACCCCCGCCAACACTTGGGGGATCACCCGCACCAGCCCCCGTGCCTGACCCCCCCGGG‑CG‑CGCCCCCCCCCCCCCACCCACCCGCCGGCCAGGCGCGGccg                                                                                                     >  2:176083/1‑131 (MQ=255)
                                   ccAATACT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACCCCCGCAACCGCCCCGGTGCCTGCCGCCCCCCGG‑CG‑CTCCCCCCCCCCCACACCCCCCAGGCGGCCATGCGCGGCagcg                                                                                                   >  4:74813/1‑133 (MQ=255)
                                   ccAATACT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCCGGG‑CG‑CTCCCCCCCCCCCACCCCGCCCAGGCGGCCCTGCTCGGCacag                                                                                                   >  2:249704/1‑135 (MQ=255)
                                   ccAATACT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCCACACCGCCCACGCCGCCCTTCTCGGCagcg                                                                                                   >  6:247960/1‑133 (MQ=255)
                                         cT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCCGGG‑CG‑CTCCCCCCCCCCCACACCCCCCCGGCGGCCCTGCTCGGCAGAGCGATCa                                                                                             >  1:273351/1‑135 (MQ=255)
                                                cccATGCCGATATCAACAAAACCCCCGCCAACCCTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CGCCCCCCCCCCCACACCCCCCACGCGGCCCAGCTCGGCAGCGCGCTCAGCaaca                                                                                       >  7:25940/1‑133 (MQ=255)
                                                cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCCGGG‑CG‑CGGCCCCCCCCCCACCCCCCCCCCGCGGCCATGCTCCGCAGAGCGATCAGCAAGa                                                                                       >  7:356391/1‑135 (MQ=255)
                                                cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCCGG‑CG‑CGCCCCCCCCCCCACACCCCCCAGGCGGCCAATCTCCGCAGAGCGAGCAGCaac                                                                                        >  6:359201/1‑133 (MQ=255)
                                                   aTGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCCGCCACCGGG‑CG‑CGCCCCCCCCCCCCCCCCGCCCCGGCGGCCCGGCGCGGCAGAGCGCTCAGCAAGcaca                                                                                    >  1:45097/1‑135 (MQ=255)
                                                     gCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCCGTGCCTGCAGCCACCGGG‑CG‑CGCCCCCCCCCCAACACCCCCCACGCGGCCATGCTCGGCAGCGCGCTCAGCAAGAACAcc                                                                                  >  7:114439/1‑135 (MQ=255)
                                                       cGATATCAACAAAACCCCCGCCAACAGTTGGTTGAACACCCGCAACCGCCCGGGGGCATGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACCCCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCa                                                                                 <  8:112110/134‑1 (MQ=255)
                                                       cGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCCGG‑CG‑CGCCCCCCCCCCCACACCCCCCCCGCGGCCATGCTCGGCAGCGCGATCACCAAGAACAcccc                                                                                >  6:56679/1‑133 (MQ=255)
                                                             caacaaAACCCCCGCCAACCCTGGGGTCAACACCCGCAACCGCCCCGGGGCCTCCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCa                                                                           <  8:263707/134‑1 (MQ=255)
                                                             caacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CGCCCCCCCCCCACCCCCCCCCCCGCGGCCCAGCTCGGCAGCGCGCGCAGCAAGAACACCACCCCCa                                                                           >  3:16325/1‑134 (MQ=255)
                                                               acaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCCGGG‑CG‑CTCCCCCCCCCCAACACCCCCCAGGCGGCCATCCTCGGCAGCGCGCTCAGCCAGCACACCAGCCCCAGCt                                                                        >  2:109656/1‑135 (MQ=255)
                                                                caaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTg                                                                       <  8:238050/135‑1 (MQ=255)
                                                                  aaaCCCCCGCCAACCCTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGcc                                                                     <  5:120695/135‑1 (MQ=255)
                                                                        ccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCACCCACCGGG‑CG‑CTCCCCCCCCCCAACCCCCCCCCCGCGGCCATGCTCGGCAGAGCGATCAGCAAGAAACCCACCGCCAGCTGCCACCCTg                                                               >  2:63668/1‑135 (MQ=255)
                                                                           ccAACACTTGGTTGAACACCCACAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CACCCCACCCCCAACACCCCCCCGCCCGCCAAGCACGGCAGCCCGATCAGCAAGAAAACCACCGCCACCTGCCCCACAGGCa                                                            >  6:160238/1‑135 (MQ=255)
                                                                           ccAACACTTCGTTGAACACCCGCAACCGCCCCGGAGCCTGCAGCCACCGGG‑CC‑CTCCCCACCCCCAACACCTCCCACCCGGCCAAGCGCGCCAGACCGATCACCAAGACCACCAACCCCACCAGCCACACAGGCa                                                            >  6:72498/1‑135 (MQ=255)
                                                                             aaCACTTGGTTGAACCCCCGCAACCGCCCCGGTGCCTGCAGCCACCCGG‑CG‑CGCCCCACCCCCCACACCCCCCACGCGGCCATGCACGGCACAGCCAACACCAAGAACACCACCCCCACCAGCCCCACAGGCAac                                                          >  3:85564/1‑135 (MQ=255)
                                                                             aaCACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCCCCCCCCACACCCCCCCGGCCGCCATGCTCGGCAGCGCCATCAGCAAGAACACCAGCCCCAGCTGCCCCCCAGGCAac                                                          >  7:88714/1‑135 (MQ=255)
                                                                                  ttGGTTGAACACCCCCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACgg                                                     <  6:128372/135‑1 (MQ=255)
                                                                                       tGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCCCCCCCAACACCGCCCCGGCGGCCCAGCACGGCAGACCGCTCACCAAGACCACCAGCCCCAGCTGCCCCACTGGCCCCGACGGcgcg                                                 >  3:173106/1‑134 (MQ=255)
                                                                                       tGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGcgcg                                                 >  8:310244/1‑134 (MQ=255)
                                                                                        gAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCCCCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCgg                                                >  4:86146/1‑134 (MQ=255)
                                                                                             cccGCAACCGCCCCGGTGCCTGCAGCCCCCGGG‑CG‑CTCCCCCCCCCCACCACCGCCCCCGCGGCCATGCTCGGCAGCGCGATCAGCAAGAACACCACCGCCACCTGCCCCCCTGGCAACGACGGCGCGGCaaaaa                                          >  6:223794/1‑133 (MQ=255)
                                                                                                   aCCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCCCCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGa                                    >  2:11460/1‑135 (MQ=255)
                                                                                                         ccGGGGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCg                              <  3:74813/135‑1 (MQ=255)
                                                                                                          cGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCCCCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGc                             >  6:300015/1‑135 (MQ=255)
                                                                                                          cGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACACCGACCACCGc                             <  8:346850/135‑1 (MQ=255)
                                                                                                           ggTGCCTGCAGCCACCGGG‑CG‑CTCCCCCCCCCCCACCCCGCCCAGGCGGCCATGCTCGGCAGAGCGATCCGCAAGCACCCCAGCGCCAGCTGCCACACTGGCCACGACGGCGCGGCAAATACCCCGCCCACCGcc                            >  6:60072/1‑135 (MQ=255)
                                                                                                           ggTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCACATAccccgccccccgcc                            >  5:107080/1‑135 (MQ=255)
                                                                                                              gCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCACCACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACg                         <  5:169163/135‑1 (MQ=255)
                                                                                                              gCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACg                         <  4:85564/135‑1 (MQ=255)
                                                                                                               ccTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACgg                        <  5:359201/135‑1 (MQ=255)
                                                                                                                    aGCCACCGGG‑CG‑CGCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGCGCGCACAGCAAGAACACCAGCCCCAGCTGCCCCACTGGCAACGACGGCGCGGCAAATACCCCGACCCCCGCCACGGCCAt                    >  7:40385/1‑134 (MQ=255)
                                                                                                                     gCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCa                  <  8:356391/135‑1 (MQ=255)
                                                                                                                      cccccGGG‑CGCCCCCCCCCCCCCACCCCCCCCCCGGCGGCCATGCGCGGCAGAGCGATCAGCAAGACCCCCCGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCa                  <  1:178953/132‑1 (MQ=255)
                                                                                                                      ccACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCaa                 <  3:299759/135‑1 (MQ=255)
                                                                                                                       ccccGGGCCG‑CACCCCCCCCCCACCCCCGCCCAGGGGGCCATGCTCGGCAGAGCTAACACCACAAACCCCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCaa                 <  1:176802/133‑1 (MQ=255)
                                                                                                                          cGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGCCCACCGCCACGGCCATCAACCAg             >  3:305467/1‑135 (MQ=255)
                                                                                                                           ggg‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGa            <  5:300015/135‑1 (MQ=255)
                                                                                                                            gg‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGAc           >  7:48964/1‑135 (MQ=255)
                                                                                                                             g‑cg‑cTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAACAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGAcc          <  2:273351/135‑1 (MQ=255)
                                                                                                                               cg‑cTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCt         >  6:7145/1‑135 (MQ=255)
                                                                                                                                   tCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTgg      <  5:247960/135‑1 (MQ=255)
                                                                                                                                       cacccccaccccCGCCCCGGCGGCCATGCTCGGCAGAGCGATCAGCAAGACCACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGtt  <  1:129366/135‑1 (MQ=255)
                                                                                                                                       cACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGtt  <  4:16325/135‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
GCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACT‑GCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGG‑CG‑CTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTT  >  KT2440/2769947‑2770213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑