| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,950,201 | 0 | T | G | 59.1% | 4.9 / 15.9 | 23 | intergenic (+99/‑97) | spuC_2/puuC_1 | Putrescine‑‑pyruvate aminotransferase/NADP/NAD‑dependent aldehyde dehydrogenase PuuC |
| Reads supporting (aligned to +/- strand): ref base T (2/7); new base G (0/13); total (3/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCAAGCTGTAAGCCGCTGACAAGGGCAACGCGCTTCCCTGTGGGAGCGGGCACGCCCGCGAACACCGGCGAAGCCGGTGCCATCCACCGTGTCGCCTGATTCGCGGGCATGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTGGAGTACAAATGTACACTCTCGAATTCTGGCAACAACGCGCC > KT2440/2950091‑2950330 | ggCACGCGGTACCCCGCGTACAAGGGCCCCGCGCTCCCCTGGGGGCGGGGGCACGCCCGCGACCCCCGGCGAACCCGGTGCCATCCCCCGGGGCGCCTGATCCGCGGGCAGGCCCGCGCCCACAGAGGGAGTgcg < 8:170540/135‑1 (MQ=255)ggCAAGCGGAACGCCGCCCACAAGGCCACCGCGCTCCCCGGGGGGCGGGGCCCCGCCCGCCACCACCGGCGACGCCGGGGCCAGCCCCCGGGCCCCCTTATGCGCGGCCCGCCCCGCGCCCACAGAGGGAGTgcg < 4:320209/135‑1 (MQ=255) atccggCTTACATGGGCATGGCGATTCCCTGTGGGAGGGGGCAGGGCGGGTATCACGGGCGAAGCCGGTGCCAGACCCGGTGTCGCGTGTTTCGCGGGCATGCCGTCTCCCACAGAGGGAGTGCGGCGTGCTTGc < 8:250728/130‑1 (MQ=255) acgCCGCTGACAAGGGCAACGCGCTGCCCTGTGGGAGCGGGCACGCCCGCGAACACCGGCGAAGCCGGTGCCATCCACCGTGTCGCCTGATTCGCGGGCATGCCCGCGCCCACAGAGGGAGTGCGGCGTGCTTGc < 4:284447/133‑1 (MQ=255) accccGCGGACACGGGCAACGCGCTGCCCGGGGGGCGCGGGCCCGCCCGCCAACACCGGCGAAGCGGGGGCCATCCACCGTGGCGCCTGATGCGCGGGCAGGCCCGCCCCCACAGAGGGAGTGCGGCGTGCTTGc < 2:29948/132‑1 (MQ=255) gCCGGTGACCCGGGCACCGCGCTGCCCGGGGGGCGCGGGCACGCCCGCGACCACCGGCGACGCGGGTGCCATCCCCCGGGGCGCCGGATTCGCGGGCATGCCGGCTCCCACAGAGGGAGTGCGGCGTGCTTGCgg < 8:33505/135‑1 (MQ=255) tGACAAGGGCAACGCGCTTCCCTGTGGGAGCGGGCACGCCCGCGAACACCGGCGAAGCCGGTGCCATCCACCGTGTCGCCTGATTCGCGGGCATGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACtgt < 2:44751/135‑1 (MQ=255) ggaaacgggCACCGCGCTTCCGTG‑GGGCGTGGGCACGCCCGCGAACACCGGCGAAGCCGGTGCCATCCACCGTGTCGCCTGATTCGCGGGCATGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACtgtg < 6:231235/129‑1 (MQ=25) cAAGGGCACCGCGCTGCCCTGGGGGAGCGGGCACGCCCGCGAACACCGGCGAAGCCGGTGCCATCCACCGTGGCGCCTGATTCGCGGGCAGGCCCGCCCCCACAGAGGGAGTGCGGCGTGCTTGCGGACtgtg < 7:238829/133‑1 (MQ=25) aCGCGCTGCCGTGGGGGCGCGGGCAGGCCCGCGAACACGGGCGACGCGGGGGCCAGCCACCGGGGCCCCTGATGCGCGGGCTGGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGt < 2:66899/135‑1 (MQ=255) cACGCCCGCGACCACGGGCGCCGCCGGTGCCCCCCCCCGTGGCGCCTGTGGCGCGGGCAGGCCCGCGCCCACAGAGGGAGTGCGGCGTGCTTGCGGACGGTGTGGTACCTGTTACTTGCGCAAGACGGCCCGCCa < 2:270347/135‑1 (MQ=255) cACGCCCGCCAACACCGGCGACGCGGGTGCCAGCCACCGTGGCGCCTGATTCGCGGCCAGGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCa < 8:71941/135‑1 (MQ=255) cGCCCGCTAACCCCGGGGACGCGGGTGCCAGCCCCCGGGGCCCCTGATTCGCGGGCATGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCacg < 6:299677/135‑1 (MQ=255) cccGGCCACGGCGGGGCCAGCCCCGGGGGCGCCTGCTGCGGGGCCAGCCCCGCCCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTg < 5:104385/134‑1 (MQ=255) acCGGCTAAGCCGGTGCCAGCCACCGTGTCGCCTGATTCGCGGGCAGGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTg < 6:367005/135‑1 (MQ=255) cGGCGAAGCGGGTGCCATCCCCCGTGTCGCCTGATGCGCGGGCATGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTGGa < 7:266263/135‑1 (MQ=255) cGAAGCCGGTGCCATCCACCGTGTCGCCTGATTCGCGGGCATGCCCGCTCCCACAGAGGGAGTGCGGCGTGCGTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCCCGCCGGTCGTTTGGAGt > 8:37934/1‑134 (MQ=255) cgggTGCCAGCCCCCGGGTCGCCTGATTCGCGGGCAGGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTGGAGTACAAAt < 8:343709/133‑1 (MQ=39) ccGTTGCCATCCCCCGGCGCGCCTTTTTGGAGGCTAGGCCGGCGCCCACAGAGGGAGTGCGGCGTGCTTGCGGAGTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTGGAGTACAAAt < 7:361047/135‑1 (MQ=255) gtCGCCTGCTTCGGGTGCAAGCCCGCTCCCTCAGAGGGAGTGCGGCCTTCTGGGGGCCTGTGTGGTTCCAGTTAGTGGCGCAAGAAGGACCGCCAAGGCGGGCCGTTTGCGAAGAAAAGTACCCTCACCAATTCt > 6:287526/1‑135 (MQ=255) cGCCTGATTCGCGGGCATGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGCCTGTGTGGTACCTGTTACGTGCGCAAGAAGGACCGCCACGACGGTCGTTTTGTGTACAAATGTACCCTCTCGGATTCTgg > 8:235890/1‑135 (MQ=255) tccgcgGGCAGGCCCGCCCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTGGAGTACAAATGTACACTCTCGAATTCTGGcaaca < 6:166876/131‑1 (MQ=255) gggCAGGCCCGCACCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTGGAGTACAAATGTACACTCTCGAATTCTGGCAACAACGCGcc < 5:174002/135‑1 (MQ=255) | GGCAAGCTGTAAGCCGCTGACAAGGGCAACGCGCTTCCCTGTGGGAGCGGGCACGCCCGCGAACACCGGCGAAGCCGGTGCCATCCACCGTGTCGCCTGATTCGCGGGCATGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTGGAGTACAAATGTACACTCTCGAATTCTGGCAACAACGCGCC > KT2440/2950091‑2950330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |