| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,316,630 | 0 | A | C | 54.5% | 4.7 / 10.6 | 11 | L107R (CTG→CGG) | CJ019_03117 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/4); new base C (0/6); total (1/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCCACCAGATCCAGCTGCAAGTGCTCAGCCAGTTCCTCTGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCCGTGCGGTCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTCCAACTGGCTCAGC > KT2440/3316506‑3316749 | ggCCACCAGATCCAGCTGCAAGTGCGCAGCCAGTTCCTCTGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCAcc < 6:301489/135‑1 (MQ=255) gCCACCAGATCCAGCTGCAAGTGCTCAGCCAGTTCCTCTGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCAcc < 4:157312/134‑1 (MQ=255) gCCACCAGATCCAGCTGCAAGTGCTCAGCCAGTTCCTCTGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCAcc < 6:1403/134‑1 (MQ=255) tGCAAGTGCTCAGCCAGTTCCTCTGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCCGTg < 7:42950/135‑1 (MQ=255) gCTCAGCCAGTTCCTCTGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCCGTgggggggg > 5:339032/1‑131 (MQ=255) gggaatgTGAGGCGCCTCGGGGGTTAGCTGCTGAATCCGCGGTTCCAGCTCAGTGCCCCCCGGGCCACGGCCCAGATTGGCGGTGGGGGCGCCTGGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAgcg < 8:344706/129‑1 (MQ=255) ggaggcgcCGCGGGGGTTAGCTGCTGGATGCGGCGTTCCAGCGCACTCCCCCGCCGGCCCCGGGCCAGCATGGCGGGGGGGCCGCCAGGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCg < 3:100044/131‑1 (MQ=255) ccggCTTGCTGCGCGGTCCCACCCCACTCCCCCGCCGGCCCCGGCCCACCATGCCCGGGGGGGCCCCTGGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCt < 3:300936/132‑1 (MQ=255) ttGAGGCGCCGTTGCAGCCCCCGCCCCCGCCGGCCCCCGGCACGAATGGCGGGGGGGGCGCCTGGGGCGCCGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTcc < 8:200307/135‑1 (MQ=255) ttGAGGCGCCGTGGCAGCCCCCGCCCCCGCCGGCCCCCGGCCCGAATGGCCGGGCGGGCGCCTGGGGCGCCGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTcc < 8:205520/135‑1 (MQ=255) ggccgCTCACTGCCCCGCCGGCCACCGCCCAGCATGGCGGGGCGGGCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTCCAACTGGCTcagc < 8:124772/131‑1 (MQ=255) | GGCCACCAGATCCAGCTGCAAGTGCTCAGCCAGTTCCTCTGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCCGTGCGGTCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTCCAACTGGCTCAGC > KT2440/3316506‑3316749 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |