Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,784,7840TG52.2% 7.7 / 20.1 23S531R (AGC→CGC) dmdC_43‑methylmercaptopropionyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (9/2);  new base G (0/12);  total (9/14)
Fisher's exact test for biased strand distribution p-value = 6.73e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCGTGTCGCAC  >  KT2440/3784696‑3784917
                                                                                        |                                                                                                                                     
agcagGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAg                                                                                          >  4:453/1‑134 (MQ=255)
 gcagGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACACCTGCAg                                                                                          >  6:84252/1‑133 (MQ=255)
     gTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCCGCACCGTTGCGCCCAACGGCAGGAACTc                                                                                    >  2:340869/1‑135 (MQ=255)
              gaagaaCCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCggcggc                                                                           >  2:165675/1‑135 (MQ=255)
               aagacCCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACAGGCAACGCCTCGATGCAGCCCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGAGGACAACAGCAGGAATTCGCCGggggcg                                                                          >  3:318200/1‑135 (MQ=255)
                               ccTGCTTTCCCCGGCGCTGCGCCGCCCCCACGGCCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTc                                                            <  8:114126/133‑1 (MQ=255)
                                     ctcccgggCGCCGCGCCGCCCCCCCTCCCACCGCCGGGAGGCAGCGCGGCCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGc                                                    <  5:37707/129‑1 (MQ=255)
                                     cgccccGGCGCGGCGCCGCCCCCACTGCCCAGGCGTGGATGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGc                                                    <  7:214031/132‑1 (MQ=255)
                                       ccccGCCGCCGCGCCGCCCCCCCGCCCACCGCCTGGAGGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGc                                                    <  5:734/132‑1 (MQ=255)
                                          cGGCGCTGCGCCGCCCCCCCTCGCACCGCCTGGAAGCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCcgcg                                               <  7:177992/135‑1 (MQ=255)
                                          cGGCGCGGCCCCGCCCCCCCTGCCACCGCGTGCAGGCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCcgcg                                               <  1:302873/135‑1 (MQ=255)
                                            gcgcGGCGCCGCCCCCCCTGCACCCGCGGGGATCCCGCGGGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGt                                             <  3:91041/135‑1 (MQ=255)
                                                tGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCCGGCGAAGGCCTGCAGCACCGTTGTGCACAACTGCAGGAAGTCGCCTGCGGCGCGGGTCGGCAAATCGGGATAGCCcgccggc                                            >  3:298577/1‑128 (MQ=255)
                                                           cACTGGCACCGCCTGGATGCCGCGCGGGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCAACAGCACCtt                              <  2:37269/135‑1 (MQ=255)
                                                                         ggATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATGGACAGCACCATGGCCCGCGCTGCCt                >  6:63015/1‑135 (MQ=255)
                                                                           aTGCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTcc              <  4:98082/135‑1 (MQ=255)
                                                                             gccgcgcgcgcGCCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAg            <  7:219092/132‑1 (MQ=255)
                                                                             gCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCGGCGGCGCGGGACGGGAATTCGGGATCGCCCGCGGGCCTCGCCAGCACCATGGCCCGCGCGGCCGCCAg            >  3:239275/1‑135 (MQ=255)
                                                                             gCACCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAg            <  6:150389/135‑1 (MQ=255)
                                                                               cgcgcgcgcGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAg            <  5:328226/132‑1 (MQ=255)
                                                                                  gcgcgcGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCgtgt       <  7:43289/135‑1 (MQ=255)
                                                                                    gcgcTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCGGCGGCGCGGGTCGGGAATTCGGGATGGCCCGCGGGGAGGGACCCCCCCATGGCCCGCGCTGCCTCCCGCGGGTcg     >  5:346605/1‑135 (MQ=255)
                                                                                       cTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCGTGTcgcac  <  6:354855/135‑1 (MQ=255)
                                                                                        |                                                                                                                                     
AGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCGTGTCGCAC  >  KT2440/3784696‑3784917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑