Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,245,1450AC56.8% ‑3.9 / 11.8 42L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (6/10);  new base C (16/5);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 2.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  KT2440/4245015‑4245265
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gcCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCCGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGGCCAACCCGGCGG‑CCCgggg                                                                                                                      >  3:343012/1‑134 (MQ=255)
 ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCCACCCGGCGG‑CCCGGgcg                                                                                                                     >  8:200904/1‑135 (MQ=255)
 ccGTCGCGTATCAGCCGCTTGGGCTGCGCAGTGGCGAAGGTCGGGCCATTGGCCCGGCGCCCGGCCCGTGCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTGGCTGCCCCACCCGGCGG‑CCCGGgcg                                                                                                                     >  1:104550/1‑135 (MQ=255)
                         tGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGAACAACCCGGCGG‑CCCGGGCGCCATACGCGCCCCAGAACacgg                                                                                               >  6:128126/1‑132 (MQ=255)
                              aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGCTCGGCGCCCCACAACTCGTCGAggca                                                                                        >  8:106379/1‑133 (MQ=255)
                              aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGa                                                                                         >  6:172062/1‑134 (MQ=255)
                                aGGCGACGGTCGGGCCTTTGGCCAGGCGCCCGGCCAGTTCGGCGGAAACGGCAGGCAACTGCTCTGCGGCGCAGAGTTCGCTGACCACCCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  4:349049/135‑1 (MQ=255)
                                 ggCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTGCGGCGGCAACGGCAGGCAAGTGCTCGGGGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  4:355066/134‑1 (MQ=255)
                                  gCGAGGGGCGGGCCATGGGCCCGGCGCCCGGCCTGTGGGGCGGCAACGGCAGGCACCTGCGCGGCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  3:121772/133‑1 (MQ=255)
                                   cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACACCTCGTCGAGGAAcagca                                                                                   >  6:275475/1‑135 (MQ=255)
                                    gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAcagcag                                                                                  >  4:63486/1‑135 (MQ=255)
                                     acgggcGGGCCATTGGCCTGGCGCCGGGCCTGTGCGGCGGCAACGGCAGGCAACTGCGCGGGGGCGCAGACTTCGCTGACCAACCCGGCGG‑GCTGGGGGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                 <  4:355497/130‑1 (MQ=255)
                                        gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCCCCACTCGTCGAGGCACAGCAGgcgc                                                                              >  8:16921/1‑135 (MQ=255)
                                        gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGATCCGCGCCCCCCAACTCGTCGAGGCACCCCCGgcgc                                                                              >  4:211455/1‑135 (MQ=255)
                                        gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGCTCGGCGCCCCCCCACTCGGCGAGGAACAGCAGgcgc                                                                              >  7:25791/1‑135 (MQ=255)
                                             gCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGCTCGGCGGCCCCCCACACGTCGAGGAACAGCAGGCGCTTGGc                                                                         >  2:28772/1‑135 (MQ=255)
                                               cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGCCG‑CCCCGGCGCGCTCGGCGCCCCACAACTCGGCGCGGCACAGCAGGCGCGTGGcca                                                                       >  7:285543/1‑134 (MQ=255)
                                               cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGCTCGGCGCCCCCCCCCTCGGCGAGGAACCGCAGGCGCGTGGCCt                                                                       >  5:141174/1‑135 (MQ=255)
                                               cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGCTCGGCGCCCCCCAACTCGTCGCGGGACAGCAGGCGCGTGGCCt                                                                       >  2:80797/1‑135 (MQ=255)
                                               cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCAGGCGCTTGGCCt                                                                       >  7:231586/1‑135 (MQ=255)
                                                  gggCCCGGCGCCCGGCCAGTGCGGCGGAAACGGCAGGCAACTGCTCGGCGGCGCATACGTGGCTGACCACCCCGGCGG‑CCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCt                                                                    <  8:7966/134‑1 (MQ=255)
                                                    ccccggCGCCCGCCAAGTGCGGCGCAAAGGGCAGCCAAGTGCCCGGCGCCGCAGACTGCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGCCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCg                                                                  <  5:85300/131‑1 (MQ=255)
                                                     ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGCCCAACCCGGCGG‑CCAGGGCGCGCTCGGCGCCCCACAACTCGTCCCGGCACCGCCGGCGCTTGGCCTGCTCGc                                                                 >  8:284199/1‑135 (MQ=255)
                                                     ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGGGACGCGGCCCCCAACGCGGGGGGGGACACCCGGCGCGTGGCCGgcgcgc                                                                 >  7:324311/1‑135 (MQ=255)
                                                          cgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGCTCGGGGCCCCACCCCTCGGCGGGGCACAGCCGGCGCGTGGGCTGCTCGCGGCCg                                                            >  1:2603/1‑135 (MQ=255)
                                                           gcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGGGCGCAGGGCGCCCCCCAACTCGTGGAGGAACACCCGGGGCGGGGCCAgcgcgcgggccg                                                           >  3:15417/1‑129 (MQ=255)
                                                            cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGATCGGCGCCCCACAACGCGGCGAGGAACAGCAGGCGCTTTGCCGGCTCGCTGCCgc                                                           >  6:183344/1‑133 (MQ=255)
                                                                   aGTTCGGCGGCAACGCCAGGCAACTGCTCGGCGGCGCAGACTGCGCTGACCAACCCGGCGG‑CCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  6:161978/135‑1 (MQ=255)
                                                                             cAAGGGCAGGCACCGGCGCGGCGGCGCAGACTGCGCTGCCCACCCCGCGGG‑CCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGcc                                         <  8:249325/135‑1 (MQ=255)
                                                                                  gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGATAGGCGCCCCCCAACTCGTCGAGGCACAGCAGGCGCTTGGGCTGCTCGCGGCCCCTCAGCCGTGGCCGGTGCCCGcgg                                    >  6:191072/1‑133 (MQ=255)
                                                                                           tGCTCGTCGGCGCAGACTTCGCGGACCACCCCGGCGG‑CCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  4:288206/135‑1 (MQ=255)
                                                                                           tGCTCGTCGGCGCAGACTCCGCTGACCACCCCGGCGG‑CCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  5:275475/135‑1 (MQ=255)
                                                                                           tGCGCGGCGCCGCAGAGTTCCCTGCCCACCCGGGCGG‑CCCGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  6:60583/135‑1 (MQ=255)
                                                                                                 gcgGCGCAGACTGCGCTGCCCACCCCGGCGG‑CCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                     <  8:336323/134‑1 (MQ=255)
                                                                                                      ccAGACTCCGCGCACCACCCCGGCGG‑CCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                <  3:217199/134‑1 (MQ=255)
                                                                                                          aCTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc            <  4:270549/135‑1 (MQ=255)
                                                                                                          aCTGCGCTCACCACCCCGGCGG‑CCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc            <  7:70722/135‑1 (MQ=255)
                                                                                                                    cccccccGGCGC‑CCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCCGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  3:283824/131‑1 (MQ=255)
                                                                                                                    ccACCCCGGCGG‑CATGGGGGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  7:189642/135‑1 (MQ=255)
                                                                                                                    ccACCCCGGCGC‑CCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  1:28772/135‑1 (MQ=255)
                                                                                                                    caaccccGGCGG‑CCGGGGCGGTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  4:343012/131‑1 (MQ=255)
                                                                                                                     ccaCCCGGGGGCCCTGGGCGCGTGCGGCCCCCCACAACTCCTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  3:271910/133‑1 (MQ=255)
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GCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  KT2440/4245015‑4245265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑