| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 3,061,793 | G→A | S592N (AGC→AAC) | qedA_2 → | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,061,793 | 0 | G | A | 97.3% | 120.5 / NA | 37 | S592N (AGC→AAC) | qedA_2 | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (26/10); minor base C (0/1); total (26/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGT > KT2440/3061661‑3061924 | tttCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg < 1:13731/135‑1 (MQ=255)tttCGGGAGGCTCCGGCGGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg < 3:234663/135‑1 (MQ=255) cGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAATGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGATGCAGCAAACGGCAAACGt > 3:270035/1‑133 (MQ=255) ggTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACCGCAAACGTCaa > 3:141573/1‑135 (MQ=255) gTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAg > 3:19724/1‑135 (MQ=255) tAGTGTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGc < 3:50723/135‑1 (MQ=255) tcCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa < 7:153690/135‑1 (MQ=255) ggCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCCACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCACACGTCAAGCCATAACgc > 8:175586/1‑135 (MQ=255) tGGGGCGGCGCGGTGCCGCTGGGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCCAGCAGTTGCAGCAAACGGCCAACCTCAAGCCATAACgcctg > 6:178111/1‑135 (MQ=255) gcgGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGcc < 4:19724/135‑1 (MQ=255) ggTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGc > 4:286853/1‑135 (MQ=255) gCCGCGGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTtg < 2:127307/135‑1 (MQ=255) gCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTtgctg < 7:250035/135‑1 (MQ=255) cgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGt > 7:344586/1‑135 (MQ=255) aaGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTg > 4:237847/1‑134 (MQ=255) aGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTgg > 1:103867/1‑134 (MQ=255) cGGGTCAAGGACTTCAACCCGGGCGGCATGCTCTGGACCTTCACGTTGCCCACGCTGTTGCAGCAAACGGCAAACGTCACGCCATACCGCCGCCCTACTGCCGCTTGCTGCCGCTGAT‑CGGGGGCAAGCGGCCct < 4:238285/135‑1 (MQ=255) ggTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCcta > 6:117923/1‑134 (MQ=255) cTTAAAACAGGGCCGCATTCATTGTACATTCAAGTTGCCCAATGATTTTGCTCAAACGGCAAACGTCAATCCATAACGCCTTGCTACAGGCGGTTTCTGCCGGTTATGTGGTGCAAGGCGGCCCTACTACCAAAt < 8:11965/135‑1 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACg > 2:273807/1‑133 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACg > 6:319263/1‑133 (MQ=255) cggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCg > 1:94062/1‑135 (MQ=255) ggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGc > 6:281002/1‑135 (MQ=255) gcAGGCGCTGCACCTCCACGTGCCCCAAGCAGTGCCAGAAAACGCCAAACGACACCCCAAACCGCCGCCCCCCGGCCGCTTGCTGCCGCTGAG‑CGGGGCCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCt < 2:33500/135‑1 (MQ=255) tGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTTCTGCCGCAGTT‑AGGTGGCCAACGGCCCGACTACCAAAAGCAGCCACCCCCGCTGcc > 6:47272/1‑135 (MQ=255) ggACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCACCGag > 7:275975/1‑134 (MQ=255) gACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGCT‑AGGTGGCACGCGGCCCTAACACCAAATGACCAGAGCCCCCCAGCCCAAGAt > 5:334479/1‑134 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGcc > 5:355756/1‑135 (MQ=255) gTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGccc > 4:320434/1‑135 (MQ=255) gTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGACACCCCCGCTGCCCACCATGCATTCGccc > 1:228367/1‑135 (MQ=255) cacgCAGTTCCCGCAAACGCCAACCGGCAAGCCATACCGCCTGCCTACTGCCGCTGGCTGCCGCTGAG‑CGGTGCCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcg < 4:150885/132‑1 (MQ=255) cAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcg > 3:318999/1‑135 (MQ=255) aaGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCACCGATGCATTCGCCCGGCAcgc > 5:100686/1‑135 (MQ=255) aaGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGCTGCATTAGCGCGGCAcgc > 8:82348/1‑135 (MQ=255) aaGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcgc > 2:285989/1‑135 (MQ=255) cgCAGTGCCACCAACCGCCAAACGCCAACCCAAAACGCCGGCCTACTGCCGCTGGCTGCCGCTGAT‑CGGGGGCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcgcg < 2:103867/134‑1 (MQ=255) agcaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGcttctt > 7:215344/1‑135 (MQ=255) aaaCGCCAAACGTCAAGCATTACCGCGGGCTTACTGCCGGTTGCGGCCGCTTAT‑GGGTGGCATGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAcc < 2:228367/135‑1 (MQ=255) cAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGt > 8:69981/1‑135 (MQ=255) | TTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAT‑CGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGT > KT2440/3061661‑3061924 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |