Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	3,440,343	A→C	S18R (AGC→CGC)	CJ019_03266 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,440,343	0	A	C	90.9%	19.6 / ‑5.9	22	S18R (AGC→CGC)	CJ019_03266	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base C (15/5);  total (17/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA  >  KT2440/3440228‑3440473                                                                                                                    |                                                                                                                                   aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGGCCGCGCGGGCGGCGCCCGGGc                                                                                                                 >  8:342287/1‑135 (MQ=255) aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCAGGCGGCGCCCCGGc                                                                                                                 >  1:92089/1‑135 (MQ=255) aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTAGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCgcgc                                                                                                                 >  5:185861/1‑135 (MQ=255)        tAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCTGGCGGCGCCCGGGCCGCCggg                                                                                                          >  3:98336/1‑135 (MQ=255)         aGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCGCCgggg                                                                                                         >  2:274011/1‑135 (MQ=255)          gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCggggg                                                                                                        >  7:79460/1‑134 (MQ=255)                gTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGcc                                                                                                  >  8:61879/1‑134 (MQ=255)                 gcgggacgaCCGCAAGGCCCTCTCCACGCGAGCGCTCGAGGGGCTGCAGGGAGCGCGTGGGCCACCCGCCGGGGCGCCGTGGCGCGGCTGCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAg                                                                                                 <  7:244908/132‑1 (MQ=255)                   tGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCTGGCGCCGCCCGGGCCCCCCGGCTGTGGCCGGc                                                                                               >  4:47697/1‑135 (MQ=255)                              aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCCGGCGGCGCCCGGGCCGCCGGGGGGTTGGCGGCCACCGCCta                                                                                      >  5:231674/1‑133 (MQ=255)                              aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCAGGCCGCGCCCGGGCCGCCGGGGTGTGGCAGGCCAACGCCta                                                                                      >  3:117987/1‑133 (MQ=255)                                   ccTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCCGGCCCACCCCGATAAGgcg                                                                               >  2:288630/1‑135 (MQ=255)                                     tctACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTGGCGGCGCCCGGGCAGCCTGGGTGTGGGAGGCCACCGCCGATAAGGCGCa                                                                             >  3:362924/1‑135 (MQ=255)                                        aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCGGGGGGTGGGAGGCCCCCCCCGATAAGGcgccgcg                                                                          >  6:132064/1‑134 (MQ=255)                                                    tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGGGGCGCCCGGGCCGCCGGGGGGTTGGAGGCCCACCCCGATAATGCGCCGCTCGCGGATCACGc                                                              >  2:222480/1‑135 (MQ=255)                                                                aGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCCGGGGGGGGGCGGCCCCCCCCGAAAAGGCGCCGCGCGCCGCTCAGGCCGCGGACTTCgg                                                  >  4:301582/1‑135 (MQ=255)                                                                     gcgcGTGGGCCCCCCGGCGGGGGGGGGGGGCGCTGCGGCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCCGGCCGATGACTTCGGCAAGc                                             <  3:304743/135‑1 (MQ=255)                                                                       gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGGCGGCCCCCGCCGAGAAGGCGCAGCGCGCGGCTCAGGCCGCGGACTTCGGCGAGCAg                                           >  4:141635/1‑135 (MQ=255)                                                                       gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGGGGCGGCCGGGGCGCCGGGGGGGGGGCGGGCCACCCCGATTATGCGCAGCGCGCGGGTCAGGGCGAGGACGTCGGcgcgcac                                           >  6:6115/1‑129 (MQ=255)                                                                                           ggCCGGGGCGCGGCTCCTGCCCGCCCGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                       <  7:267358/134‑1 (MQ=255)                                                                                                          gcgggCCGCCCGGGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCg        <  4:98336/132‑1 (MQ=255)                                                                                                                cgccCGCGCGGCCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa  <  1:288630/135‑1 (MQ=255)                                                                                                                    |                                                                                                                                   ATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA  >  KT2440/3440228‑3440473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑