Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 3,712,756 T→C intergenic (‑299/‑48) paaF_1 ← / → yrdA_3 2,3‑dehydroadipyl‑CoA hydratase/Protein YrdA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,712,7560TC98.6% 254.0 / NA 73intergenic (‑299/‑48)paaF_1/yrdA_32,3‑dehydroadipyl‑CoA hydratase/Protein YrdA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (28/44);  minor base A (1/0);  total (29/44)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGATCACAGCTCCAGTGCAATGCTTGCTGAAGAATCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCATCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCACCCCAGCGCAGTCC  >  KT2440/3712625‑3712876
                                                                                                                                   |                                                                                                                        
tATGATCACAGCTCCAGTGCAATGCTTGCTGAAGAATCGTTTAACCTTTGCATTTTCTATTACTTACAGAGGGTTTTTGCCTTGCAGCATTAATTAAACACAAGTGATACACGATTGACGCCCAAACAGCACCTg                                                                                                                       >  3:292489/1‑135 (MQ=255)
   gATCACAGCTCCAGTGCAATGCTTGCTGAAGAATCGTTTAAACTTTTCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATa                                                                                                                    <  4:167339/135‑1 (MQ=255)
     tCACAGCTCCAGTGCAATGCTTGCTGAAGAATCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATAc                                                                                                                   <  2:323103/134‑1 (MQ=255)
            tCCAGTGCAATGCTTGCTGAAGAATCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGa                                                                                                           >  7:118988/1‑135 (MQ=255)
             ccAGTGCAATGCTTGCTGAAGAATCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGAc                                                                                                          >  4:106203/1‑135 (MQ=255)
                    aaTGCTTGCTGAAGAATCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACAtt                                                                                                   >  5:203057/1‑135 (MQ=255)
                     aTGCTTGCTGAAGAATCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTc                                                                                                  <  5:195860/135‑1 (MQ=255)
                       tactgctGAATATACGTTTAACCTTTCAATTTTATCGAAATTACCGCGGGTTTTTCCCTTGAAGCATAAATTAAACAAACGTTAAAAACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCa                                                                                                <  2:365026/132‑1 (MQ=255)
                        cttgctGAAGAATCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCaa                                                                                               >  5:90683/1‑135 (MQ=255)
                        cttgctGAAGAATCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCaa                                                                                               >  6:139671/1‑135 (MQ=255)
                         ttgctGAAGAATCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACCCGATTGACGACCAAACAGCACCTGATACATGATCGACTGACATTCCaaa                                                                                              >  1:138724/1‑135 (MQ=255)
                           gctGAAGAATCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATc                                                                                            >  8:163965/1‑135 (MQ=255)
                           gctGAAGAATCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCAACTGATACAAGATCGACTGACATTCCAAATc                                                                                            >  4:42623/1‑135 (MQ=255)
                              gaagaaTCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCAtt                                                                                         >  6:75115/1‑135 (MQ=255)
                              gaagaaTCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCAtt                                                                                         >  7:319892/1‑135 (MQ=255)
                                  aaTCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCga                                                                                     <  5:13540/135‑1 (MQ=255)
                                  aaTCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCga                                                                                     <  4:241794/135‑1 (MQ=255)
                                  aaTCGATTAACCTTTGCATTATCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCga                                                                                     <  2:35285/135‑1 (MQ=255)
                                    tCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACGTGATACAAGATCGACTGACATTCCAAATCATTTCgaga                                                                                   <  8:60615/135‑1 (MQ=255)
                                    tCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCgaga                                                                                   <  6:198262/135‑1 (MQ=255)
                                       tttAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAgtg                                                                                <  5:75115/135‑1 (MQ=255)
                                          aaCCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGagagctgt                                                                              >  1:365026/1‑130 (MQ=255)
                                          aaCCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTg                                                                             <  1:100495/135‑1 (MQ=255)
                                            ccTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGcc                                                                           <  4:154623/135‑1 (MQ=255)
                                              tttGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAACCAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCAt                                                                         <  7:90565/135‑1 (MQ=255)
                                              tttGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCAt                                                                         <  2:139919/135‑1 (MQ=255)
                                              tttGCATTTTCTAGTACTTACAGCGGGGTGTTGCCTTGCAGCATTTATTCAACACCAGTGTTACTCGATTGACGACCAAACAGCACCTGATACAAGATCGACTGTCATTCCAAATCATTTCGAGAGTGTTGCGAt                                                                         <  2:353266/135‑1 (MQ=255)
                                              tttGCATTTACTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGAGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCAt                                                                         <  5:70307/135‑1 (MQ=255)
                                               ttGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTg                                                                                                        <  8:190451/103‑1 (MQ=255)
                                               ttGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTg                                                                                                        >  7:190451/1‑103 (MQ=255)
                                                   aTTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCtt                                                                    >  6:290110/1‑135 (MQ=255)
                                                     tttCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGc                                                                  <  6:203057/135‑1 (MQ=255)
                                                          aGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCTACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCg                                                             >  1:241104/1‑135 (MQ=255)
                                                             aCTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACt                                                          <  1:235033/135‑1 (MQ=255)
                                                                   aGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACgg                                                    <  7:237715/135‑1 (MQ=255)
                                                                    gCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCAt                                                                         <  2:267261/113‑1 (MQ=255)
                                                                    gCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCAt                                                                         >  1:267261/1‑113 (MQ=255)
                                                                    gCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTAt                                                               <  5:220860/123‑1 (MQ=255)
                                                                    gCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTAt                                                               >  6:220860/1‑123 (MQ=255)
                                                                    gCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGc                                                   >  3:96886/1‑135 (MQ=255)
                                                                    gCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGc                                                   >  7:55863/1‑135 (MQ=255)
                                                                    gCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGc                                                   >  6:251979/1‑135 (MQ=255)
                                                                     cGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGcc                                                  <  8:315558/135‑1 (MQ=255)
                                                                     cGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGcc                                                  <  4:289161/135‑1 (MQ=255)
                                                                      gggTTTTTGCGTTTCAGCATTAATTCAACACAAGTGATACATGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCt                                                 <  2:190039/135‑1 (MQ=255)
                                                                          ttttGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCTACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGCCg                                             >  4:170349/1‑135 (MQ=255)
                                                                              gCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCt                                          <  4:226543/134‑1 (MQ=255)
                                                                                     agcaTTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCa                                  <  8:242106/135‑1 (MQ=255)
                                                                                      gcaTTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCAc                                 >  7:317308/1‑135 (MQ=255)
                                                                                       caTTAATTCAACACATGTGATAAACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCAcc                                <  2:298707/135‑1 (MQ=255)
                                                                                       caTTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCAcc                                <  8:180941/135‑1 (MQ=255)
                                                                                       caTTAATTCAACACAAGGTATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCAcc                                <  7:315262/135‑1 (MQ=255)
                                                                                          tAATTCAACACAAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGa                             <  2:303297/135‑1 (MQ=255)
                                                                                                  cacaAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTAc                     <  2:240309/135‑1 (MQ=255)
                                                                                                  cacaAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTAc                     <  7:4127/135‑1 (MQ=255)
                                                                                                    caAGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGt                   >  5:59446/1‑135 (MQ=255)
                                                                                                     aaGTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGt                   >  3:148124/1‑134 (MQ=255)
                                                                                                       gTGATACACGATTGACGACCAAACAGCCCCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCa                <  6:130541/135‑1 (MQ=255)
                                                                                                       gTGATACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCa                <  4:95195/135‑1 (MQ=255)
                                                                                                       gTGATACACGATTGACGACAAAACAGCACCTGATACAATATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCa                <  4:90109/135‑1 (MQ=255)
                                                                                                          aTACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCAcc              >  1:257757/1‑134 (MQ=255)
                                                                                                          aTACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCAcc              >  4:361102/1‑134 (MQ=255)
                                                                                                          aTACACGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGAGTTGCCATGCCTTGCTATCGACTGGACGTCCTGACGCCTGTCGTTCACCAGACAGCCTACGTGCAccc             >  5:292021/1‑135 (MQ=255)
                                                                                                            acacGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCACCCCa           <  8:249548/135‑1 (MQ=255)
                                                                                                            acacGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCACCCCa           <  8:319047/135‑1 (MQ=255)
                                                                                                            acacGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCACCCCa           <  5:139671/135‑1 (MQ=255)
                                                                                                            acacGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCACCCCa           <  4:7681/135‑1 (MQ=255)
                                                                                                            acacGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGGTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCACCCCa           <  6:344205/135‑1 (MQ=255)
                                                                                                            acacGATTGACGACCAAACAGCACCTGATACAAGAGCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCACCCCa           <  6:240450/135‑1 (MQ=255)
                                                                                                              acGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCACCCCAgc         <  3:284617/135‑1 (MQ=255)
                                                                                                              acGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCACCCCAgc         <  1:114375/135‑1 (MQ=255)
                                                                                                               cGATTGACGACCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCACCCCAgc         >  5:69469/1‑134 (MQ=255)
                                                                                                                     acgacCAAACAGCACCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCACCCCAGCGCAGTcc  >  4:359477/1‑135 (MQ=255)
                                                                                                                                   |                                                                                                                        
TATGATCACAGCTCCAGTGCAATGCTTGCTGAAGAATCGTTTAACCTTTGCATTTTCTAGTACTTACAGCGGGTTTTTGCCTTGCAGCATTAATTCAACACAAGTGATACACGATTGACGACCAAACAGCATCTGATACAAGATCGACTGACATTCCAAATCATTTCGAGAGTGTTGCCATGCCTTGCTATCGACTGGACGGCCTGACGCCTGTGGTTCACCCGACAGCCTACGTGCACCCCAGCGCAGTCC  >  KT2440/3712625‑3712876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: