Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 3,956,826 G→C H275D (CAT→GAT)  dmlR_13 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,956,8260GC97.2% 240.3 / NA 72H275D (CAT→GAT) dmlR_13HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (31/39);  minor base A (2/0);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGCGGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATGGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGCCAATGCCCAT  >  KT2440/3956696‑3956949
                                                                                                                                  |                                                                                                                           
aCCGCGGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGt                                                                                                                         >  7:180610/1‑135 (MQ=255)
 ccGCGGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTg                                                                                                                        >  7:243262/1‑135 (MQ=255)
  cgcgGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGa                                                                                                                       <  3:236719/135‑1 (MQ=255)
    ctgCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGTCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                     <  7:345525/133‑1 (MQ=255)
    cgGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGATGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                     <  3:11206/135‑1 (MQ=255)
    cgGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                     <  2:6019/135‑1 (MQ=255)
    cgGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                     <  6:331513/135‑1 (MQ=255)
    cgGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCGCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                     <  6:110170/135‑1 (MQ=255)
      gCAAACTTCTCGCCTCAGCGCCAATACCGCGAATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                     <  5:38520/133‑1 (MQ=255)
       cAAACTTCTCGCCTCAGCGCCAATACCGCGCATGATCCACCACCCTGGCATGCCCCTCGAACAGCTCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGAt                                                                                                                  <  4:336062/135‑1 (MQ=255)
         aaCTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATAc                                                                                                                <  2:299506/135‑1 (MQ=255)
            ttCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATc                                                                                                             <  2:228015/135‑1 (MQ=255)
               tcGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGCCGGATACATCGc                                                                                                           >  1:250791/1‑134 (MQ=255)
               tcGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGc                                                                                                           <  1:307244/134‑1 (MQ=255)
               tcGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGc                                                                                                           <  3:142680/134‑1 (MQ=255)
                  ccTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCAt                                                                                                         <  8:315338/133‑1 (MQ=255)
                  ccTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCAt                                                                                                         <  2:118498/133‑1 (MQ=255)
                  ccTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCAt                                                                                                         <  1:287128/133‑1 (MQ=255)
                    tCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAg                                                                                                     >  3:363354/1‑135 (MQ=255)
                     tcaggcCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAgc                                                                                                    <  4:17632/131‑1 (MQ=255)
                       gcgcCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAgc                                                                                                    <  4:200660/133‑1 (MQ=255)
                           cAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGCCGGATACATCGCATACAGCGCActc                                                                                              >  1:337146/1‑135 (MQ=255)
                             aTACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACtctc                                                                                            <  8:180610/135‑1 (MQ=255)
                              tACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTcc                                                                                           <  1:81948/135‑1 (MQ=255)
                                 cgcgCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGc                                                                                        <  3:73106/135‑1 (MQ=255)
                                     catcatCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACCTCCCATACAGCGCACTCTCCCGCAGcc                                                                                    >  2:361793/1‑135 (MQ=255)
                                     catcatCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACCGCGCCCTCTCCCGCAGcc                                                                                    >  1:348676/1‑135 (MQ=255)
                                     catcatCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACCGCGCACTCTCCCGCAGc                                                                                     >  5:202996/1‑134 (MQ=255)
                                     catcatCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGc                                                                                     >  7:294195/1‑134 (MQ=255)
                                     catcatCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGGGACGGATACATCGCATACAGCGCACTCTCCCGCAGcc                                                                                    >  7:140013/1‑135 (MQ=255)
                                     catcatCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGAGCCAGGTGCGCCCCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGGATACCGCGCACTCTCCCGCAGcc                                                                                    >  4:218398/1‑135 (MQ=255)
                                     catcatCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTCAGGTAGTCGATCCAGGTGCGCCCCTTGGCGTCCACGTAATAGCCGGACGGCTACCACGCATACCCCCCACTCACCCGCacc                                                                                     >  6:237297/1‑132 (MQ=255)
                                        catcCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGAt                                                                                 <  7:101789/135‑1 (MQ=255)
                                                   cTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAAt                                                                      <  3:157847/135‑1 (MQ=255)
                                                       cATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                     <  6:238749/84‑1 (MQ=255)
                                                       cATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                     <  6:234083/84‑1 (MQ=255)
                                                       cATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                     >  5:238749/1‑84 (MQ=255)
                                                       cATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTg                                                                           <  5:111982/126‑1 (MQ=255)
                                                       cATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAAcc                                                                  >  2:36054/1‑135 (MQ=255)
                                                       cATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCACGCAGCCGATACGGTg                                                                           >  6:111982/1‑126 (MQ=255)
                                                       cATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATAACTCGCAAAAAGCGCACTCTCACGCAGCCGATACGGTGCCAATAAcc                                                                  >  1:359123/1‑135 (MQ=255)
                                                       cATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGAGTGACg                                                                                                                     >  5:234083/1‑84 (MQ=255)
                                                       cATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTAGGCGTCCAGGTAATCGCGTGACGGATACATAGCATACAGCGCACTCTCCCGCAGCCGAAACGGTGCCAATAAcc                                                                  >  4:275391/1‑135 (MQ=255)
                                                         tGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGAGACGGTGCCAATAACCgg                                                                >  1:179310/1‑135 (MQ=255)
                                                               tCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATa                                                                                                        <  6:72426/89‑1 (MQ=255)
                                                               tCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATa                                                                                                        >  5:72426/1‑89 (MQ=255)
                                                                   aCAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTc                                                      >  4:232939/1‑135 (MQ=255)
                                                                          cGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGca                                                              >  2:282363/1‑120 (MQ=255)
                                                                          cGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGca                                                              <  1:282363/120‑1 (MQ=255)
                                                                          cGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGccc                                               <  1:226052/135‑1 (MQ=255)
                                                                                    tCCTTGAGGTAGGCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCg                                     <  4:221590/135‑1 (MQ=255)
                                                                                          aGGTAGTCGATCCAGGAGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCTCAGCCGCTACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCg                               <  4:341961/135‑1 (MQ=255)
                                                                                           ggTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCgg                              >  6:170835/1‑135 (MQ=255)
                                                                                             tAGTCGATCCAGGTGCGCACCTTGGCGGCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGgtg                            <  8:140013/135‑1 (MQ=255)
                                                                                                  gATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGaa                       >  8:235309/1‑135 (MQ=255)
                                                                                                   aTCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTGCCTGCCCGATCGCCTCGCTGGCGGTGTAGAAc                      >  4:102057/1‑135 (MQ=255)
                                                                                                   aTCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGc                                    >  5:246430/1‑121 (MQ=255)
                                                                                                   aTCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGc                                    <  6:246430/121‑1 (MQ=255)
                                                                                                   aTCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAAc                      >  1:246055/1‑135 (MQ=255)
                                                                                                   aTCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAAc                      >  1:143462/1‑135 (MQ=255)
                                                                                                     acAGCTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACGCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTCCCCGATCGCCTCGCTGGCTGTGTAGAACgg                    <  7:94296/134‑1 (MQ=255)
                                                                                                       aGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGgca                  >  1:32452/1‑135 (MQ=255)
                                                                                                       aGGTGCGCACCTTGGCGTCCACGTAATAGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTAGCTGGCGGTGTAGAACGgca                  >  3:196357/1‑135 (MQ=255)
                                                                                                            cgcACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAgg             <  3:232939/135‑1 (MQ=255)
                                                                                                             gcACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGc            <  8:243262/135‑1 (MQ=255)
                                                                                                             gcACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCAGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGc            <  6:299664/135‑1 (MQ=255)
                                                                                                             gcACCTTGGCGTCCAGGGAATCGCGTGACGGATACATGGCATACAGCGCACTCTCCCGCAGCCGATACGCTGCCAATAACCGGCACAAGCGTCCCAGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAgg             <  3:275391/134‑1 (MQ=255)
                                                                                                                 cTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCGTACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGCCAat        >  1:110523/1‑135 (MQ=255)
                                                                                                                 cTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGCCAat        >  1:207626/1‑135 (MQ=255)
                                                                                                                    ggCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGCCAatgc      >  1:318074/1‑134 (MQ=255)
                                                                                                                       ggccAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGCCAatgcccat  <  1:149701/133‑1 (MQ=255)
                                                                                                                       gTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCTGTGTAGAACGGCAGCAGGCCAatgcccat  <  4:58092/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                           
ACCGCGGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATGGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGCCAATGCCCAT  >  KT2440/3956696‑3956949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: