Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	4,959,063	G→A	Q40* (CAG→TAG)	norR_4 ←	Anaerobic nitric oxide reductase transcriptionregulator NorR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,959,063	0	G	A	100.0%	202.7 / NA	61	Q40* (CAG→TAG)	norR_4	Anaerobic nitric oxide reductase transcriptionregulator NorR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/12);  total (49/12)

TAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGCAATAGC  >  KT2440/4958958‑4959188                                                                                                          |                                                                                                                              tAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGtttt                                                                                                  >  6:301494/1‑135 (MQ=255)  aGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTttc                                                                                                 >  8:355449/1‑135 (MQ=255)      aCTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCg                                                                                             >  6:234005/1‑135 (MQ=255)             tAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCagag                                                                                        >  1:59729/1‑133 (MQ=255)                 cagcCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGtt                                                                                  <  8:323080/135‑1 (MQ=255)                 cagcCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGtt                                                                                  <  8:49359/135‑1 (MQ=255)                    cccAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAg                                                                               <  4:133706/135‑1 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACCGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                            >  8:158056/1‑135 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCAtggc                                                                                                                    <  5:263933/95‑1 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCAtggc                                                                                                                    >  6:263933/1‑95 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCg                                                                                          >  1:361816/1‑121 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCg                                                                                          <  2:361816/121‑1 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCCGcac                                                                            >  6:321830/1‑135 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                            >  4:23841/1‑135 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                            >  4:83804/1‑135 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                            >  3:88599/1‑135 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                            >  4:251492/1‑135 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                            >  6:302344/1‑135 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                            >  7:302449/1‑135 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                            >  1:365041/1‑135 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGca                                                                             >  3:172543/1‑134 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGca                                                                             >  4:118959/1‑134 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGca                                                                             >  8:102706/1‑134 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGACTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                            >  1:200008/1‑135 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACCCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                            >  2:112384/1‑135 (MQ=255)                       aaGCAGATTGCCCGGAGCATTCACGGTGAAGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGCCCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGca                                                                             >  1:278045/1‑134 (MQ=255)                       aaGCAGATTGCCCGGAGCATTAACGGTGCCGATGAGCACGCCCAGCACTTCGCGGCTCGAGGACACTGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcac                                                                            >  8:247242/1‑135 (MQ=255)                       aaGCAGAGTGCCCGGAGACTTCACGGTGCCGATGAGCACGCCCAGCACTTCGCGGCTAGCGTACAATTGCTCGACCTCCTGCTACCAGTCAGGTCTGGAACACGCGAGTGTGTCGTCGCGGAGGAAGTgcagca                                                                             >  6:321827/1‑134 (MQ=255)                          cAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcaccac                                                                         >  1:334697/1‑135 (MQ=255)                          cAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcaccac                                                                         >  1:342966/1‑135 (MQ=255)                            gATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGa                                                                                         <  4:277498/117‑1 (MQ=255)                            gATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGa                                                                                         >  3:277498/1‑117 (MQ=255)                                 cccGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAgg                                                                  >  5:192272/1‑135 (MQ=255)                                 cccGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAgg                                                                  >  4:207048/1‑135 (MQ=255)                                 cccGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAgg                                                                  >  5:304379/1‑135 (MQ=255)                                      aGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTcgcg                                                             >  6:305449/1‑135 (MQ=255)                                              cGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGgcgcg                                                     >  8:314283/1‑135 (MQ=255)                                               ggTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGCCCGCCTGCTACCAGTCATGGCTGGCTCCCGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGgcgcgc                                                    >  6:134821/1‑135 (MQ=255)                                                   ccGATGAGCACGCACAGCACTTCGCGGCTCGAGGTCAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTg                                                >  1:6207/1‑135 (MQ=255)                                                     gATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTgtc                                              >  1:278625/1‑135 (MQ=255)                                                      aTGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTgtcg                                             >  5:315419/1‑135 (MQ=255)                                                           cacgcacAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATc                                        <  7:355449/135‑1 (MQ=255)                                                              ccacaGCACAACGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGACATGGCTGGCACGCGCGAGGTGATCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGCCGCGCGCTGTCGGCCTCggt                                     >  2:105703/2‑133 (MQ=255)                                                                acaGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATca                                   >  3:351602/1‑135 (MQ=255)                                                                       ttCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCGTCGCTCAGCAGaa                             >  5:292463/1‑134 (MQ=255)                                                                       ttCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAAt                            >  6:101018/1‑135 (MQ=255)                                                                            ggCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTgg                       >  5:190917/1‑135 (MQ=255)                                                                            ggCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTgg                       >  5:207837/1‑135 (MQ=255)                                                                            ggCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTCTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGAt                                     <  2:350444/121‑1 (MQ=255)                                                                            ggCTCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTCTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGAt                                     >  1:350444/1‑121 (MQ=255)                                                                               tCGAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGttt                    >  8:35212/1‑135 (MQ=255)                                                                                 gAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGgcgc                                                      >  6:87185/1‑99 (MQ=255)                                                                                 gAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGgcgc                                                      <  5:87185/99‑1 (MQ=255)                                                                                 gAGGACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGAGCAGCAGAATCTTGGTTTCa                  >  6:28280/1‑135 (MQ=255)                                                                                   ggACAATGGCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACg                <  2:59729/135‑1 (MQ=255)                                                                                           gCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATg         >  8:202968/1‑134 (MQ=255)                                                                                           gCTCGACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATg         >  3:348575/1‑134 (MQ=255)                                                                                               gACCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGCAATa    >  5:235696/1‑135 (MQ=255)                                                                                                aCCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGCAAt     <  7:191525/133‑1 (MQ=255)                                                                                                aCCGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGGCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGCAATAg   <  5:234005/135‑1 (MQ=255)                                                                                                 ccGCCTGCTACCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCGGTCGTCCTCGATCAGCAGCATCTTGGTTTGCCGCCACATGCAATAGc  >  5:293070/1‑135 (MQ=255)                                                                                                          |                                                                                                                              TAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTGGTTTCACGCCACATGCAATAGC  >  KT2440/4958958‑4959188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑