Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 120,983 (T)9→8 intergenic (+280/+92) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440120,9750T.94.0% 270.4 / 14.6 70intergenic (+272/+100)polA/engBDNA polymerase I/putative GTP‑binding protein EngB
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base . (42/21);  total (44/26)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCAGAGTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATCCA  >  KT2440/120841‑121107
                                                                                                                                      |                                                                                                                                    
cTCAGAGTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGttt                                                                                                                                    >  4:190738/1‑137 (MQ=255)
        aaCTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGttttttttgcc                                                                                                                            >  6:51870/1‑134 (MQ=255)
             aaTGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGaa                                                                                                                      >  7:172086/1‑137 (MQ=255)
                ggCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAAttt                                                                                                                   <  3:190738/137‑1 (MQ=255)
                    ggTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCg                                                                                                               <  5:47410/137‑1 (MQ=255)
                      tGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCggg                                                                                                             <  4:262802/137‑1 (MQ=255)
                       gAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGa                                                                                                            >  1:49598/1‑137 (MQ=255)
                        aaGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAg                                                                                                           >  3:157210/1‑137 (MQ=255)
                              ttCGATGCTCCTATGTTCTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCCGACTTGGACACCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCTGAATTTGCCGGGAGCGGGtt                                                                                                     >  8:145441/1‑137 (MQ=255)
                                        cTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCACCCTCCCAATACGAAGTCCGGGGTTTTTTTTTCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGaa                                                                                           >  1:234549/1‑137 (MQ=255)
                                         tATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                          <  4:302879/137‑1 (MQ=255)
                                         tATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                          <  5:16493/137‑1 (MQ=255)
                                           tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCTGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGc                                                                                        <  5:350621/137‑1 (MQ=255)
                                           tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGc                                                                                        <  1:5778/137‑1 (MQ=255)
                                                  ttAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGACGGGAGCGGGTTTTCTCGCGAATGCGATGTCg                                                                                 >  4:230127/1‑137 (MQ=255)
                                                   tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                          <  3:108617/127‑1 (MQ=255)
                                                   tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                          >  4:108617/1‑127 (MQ=255)
                                                   tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGa                                                                                >  7:49525/1‑137 (MQ=255)
                                                   tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGa                                                                                >  7:254657/1‑137 (MQ=255)
                                                   tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGa                                                                                >  2:22074/1‑137 (MQ=255)
                                                   tAAGTGTTGGCAGATATCTGGACCCCGCACTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGCTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTATCGCGAATGCGATGTCGa                                                                                >  7:173949/1‑137 (MQ=255)
                                                    aaGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCTGGGGGTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGaa                                                                               >  1:330706/1‑137 (MQ=255)
                                                     aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAAt                                                                              >  5:98043/1‑137 (MQ=255)
                                                     aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAAt                                                                              >  3:257511/1‑137 (MQ=255)
                                                     aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAAt                                                                              >  3:61149/1‑137 (MQ=255)
                                                     aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCAGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTATCGCGAATGAGATGTCGatt                                                                              >  4:113462/1‑135 (MQ=255)
                                                        gtTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATaca                                                                           >  1:100614/1‑137 (MQ=255)
                                                                     tGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCTAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTCCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTcgc                                                              <  6:216563/137‑1 (MQ=255)
                                                                           ccGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTaa                                                        <  8:254657/137‑1 (MQ=255)
                                                                               ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGATTTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAccc                                                    >  2:5168/1‑137 (MQ=255)
                                                                               ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTACAccc                                                    >  1:130634/1‑137 (MQ=255)
                                                                               ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAccc                                                    >  6:204948/1‑137 (MQ=255)
                                                                               ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAccc                                                    >  6:320855/1‑137 (MQ=255)
                                                                               ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAccc                                                    >  6:143948/1‑137 (MQ=255)
                                                                               ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAccc                                                    >  3:317131/1‑137 (MQ=255)
                                                                               ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAccc                                                    >  8:28146/1‑137 (MQ=255)
                                                                               ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAccc                                                    >  3:211005/1‑137 (MQ=255)
                                                                               ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAccc                                                    >  2:299867/1‑137 (MQ=255)
                                                                               ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCAGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAccc                                                    >  2:231881/1‑137 (MQ=255)
                                                                                 tAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCgg                                                  <  3:86345/137‑1 (MQ=255)
                                                                                  aGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCggg                                                 >  5:43046/1‑137 (MQ=255)
                                                                                     ggTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCa                                              >  2:81453/1‑137 (MQ=255)
                                                                                      gTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGATTGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAg                                             >  8:145170/1‑137 (MQ=255)
                                                                                      gTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAg                                             >  4:272338/1‑137 (MQ=255)
                                                                                        ccGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                           <  6:86413/137‑1 (MQ=255)
                                                                                         cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgcg                                          <  5:204948/137‑1 (MQ=255)
                                                                                           gACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCa                                        <  7:44459/137‑1 (MQ=255)
                                                                                            aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCaa                                       >  8:11880/1‑137 (MQ=255)
                                                                                            aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCaa                                       >  2:45202/1‑137 (MQ=255)
                                                                                            aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCaa                                       >  7:240260/1‑137 (MQ=255)
                                                                                                   ccccGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTa                                >  3:295633/1‑137 (MQ=255)
                                                                                                    cccGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATACGCGGGTAAACCCGGGTCCGGCGCAAACCACTaa                               >  6:102759/1‑137 (MQ=255)
                                                                                                         aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGAc                          >  8:49171/1‑137 (MQ=255)
                                                                                                         aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGAc                          >  4:68428/1‑137 (MQ=255)
                                                                                                         aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGAc                          >  2:37460/1‑137 (MQ=255)
                                                                                                         aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGGCGAATACACCGCCGCATTCGCCGGTATACCCGGGTCAGGCGCAAACCACTAAACGAc                          >  1:179902/1‑137 (MQ=255)
                                                                                                         aCTTCCCCCTCCCCATACGAAGTCCGGGGTGTTTTTTGCCCGGAATTAGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCATCCCACTAAACgcc                          >  8:178185/1‑135 (MQ=38)
                                                                                                          attCCCCCTCCCCTTACGAAGTCGGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGGGGGTTTTCTCGCTAATGCTATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGAcc                         <  3:112002/136‑1 (MQ=255)
                                                                                                             cccccTCCCCTTACTAAGGCCGTGTTTTTTTTTGCCCTGAATTTGCCGGGAGCGGGTTTTCTGGGTAATGGTTTGTCGAATACACCGCCTCATTCGCGTGTAAACCCGGGTCAGGCTAAAACCACTAAACGACCGGc                      <  8:173949/137‑1 (MQ=255)
                                                                                                             cccccTCCCCATAGGAAGTCCGGGGTTTTTTTTGCCCGTAATTTGCCGGGAGGGGGTTTTCTCGCGAATGCTATGTCGAATACACCGCCGCATTCGCGGTTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGc                      <  4:46029/137‑1 (MQ=255)
                                                                                                             cccccTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCTAATACACCGCCGAATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGc                      <  6:122726/137‑1 (MQ=255)
                                                                                                             cccccTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGc                      <  1:22074/137‑1 (MQ=255)
                                                                                                             cccccACCCAATACTAAGTCGGGGGTTTTTTTTGCCCGTATTTTGCGGGTCGGGGGTTTTCTCGCTAATGGGATGTCGAATACACCGCCGCATTCGCGGGTAAACCGGGGTCAGGCGCAAACCACTAAACGACCGGc                      <  2:330706/137‑1 (MQ=255)
                                                                                                                 cTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGt                  >  7:103383/1‑137 (MQ=255)
                                                                                                                  tCCCCATCAGACGTCGGGGTTTTTTTTTGCCGGGAATTTGCCGGGGGGGGTTTTGCTCGCGAAGGCGATGTGTAATACACCGCCGCATTCGCGGGTAAACCGGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTc                 <  2:234549/137‑1 (MQ=255)
                                                                                                                  tCCCAATCCGAGGTCCGGGGTTTTTTTTGCCTGGAATTTGCCGGGTGCGGTTTTTCTGGCTAATGCGTTGTCTAATACACCGCCGCATTCGCGGGTAAACCCGGTTCAGGCGCAAACCACTACACGACCGGCTTGTc                 <  4:234569/137‑1 (MQ=255)
                                                                                                                  cccccATAGGAAGTCCGGGGTTTTTTTTGCCCTGAATTTGCCGGGTGCGGGTTTTCTCGCTAAGGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGGCAGGCGCAAACCACTAAACGACCGGCTTGTc                 <  3:334012/136‑1 (MQ=255)
                                                                                                                     ccATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAACCCACTAAACGACCGGCTTGTCttg              >  1:346087/1‑136 (MQ=255)
                                                                                                                      cATACTAAGGCCGGGTTTTTTTTTGCCGGTTATTTGGGGGGTGGGGGTTTTCTCGGTAATGCGATGTCGAATACACCGCGGGTTGGGCGGGTAAACCGGGGTCCGGCGAAAACCCATCAACGACCGGCTTGTCTTcc             <  8:128629/137‑1 (MQ=255)
                                                                                                                       aTACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCa            <  4:307567/137‑1 (MQ=255)
                                                                                                                       aTACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCa            <  8:36400/137‑1 (MQ=255)
                                                                                                                            aaGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACAACTAAACGACCGGCTTGTCTTCCAGTtcc       >  4:128413/1‑137 (MQ=255)
                                                                                                                                 ccggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatcc   >  7:33149/3‑137 (MQ=255)
                                                                                                                                 ccggggTTTTTTATGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTAGCGGGTAACCCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatcc   >  7:133388/3‑137 (MQ=255)
                                                                                                                                  cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatcca  >  7:340224/2‑137 (MQ=255)
                                                                                                                                  cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatcca  >  2:38558/2‑137 (MQ=255)
                                                                                                                                  cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatcca  >  5:59995/2‑137 (MQ=255)
                                                                                                                                  cggggTTTTTTTTGCCCGGAATTTGCCGGGAGAGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGCTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCCGTtccatcca  >  7:128629/2‑137 (MQ=255)
                                                                                                                                      |                                                                                                                                    
CTCAGAGTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATCCA  >  KT2440/120841‑121107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: