Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	763,257	0	T	G	25.9%	97.0 / 13.0	58	F267C (TTT→TGT)	gcvA_4	Glycine cleavage system transcriptionalactivator
Reads supporting (aligned to +/- strand):  ref base T (5/38);  new base G (15/0);  total (20/38)
Fisher's exact test for biased strand distribution p-value = 5.21e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGTCGACCCTGCGCATGGCGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTGGAGCACCGATGCAGTACACATCCAC  >  KT2440/763128‑763383                                                                                                                                  |                                                                                                                               tgtCGACCCTGCGCATGGCGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGGTGTTCCTg                                                                                                                         >  7:67298/1‑137 (MQ=255) ggtCGACCCTGCGCATGGCGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTg                                                                                                                         <  8:214299/136‑1 (MQ=255)   tCGACCCTGCGCATGGCGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTGCTGGt                                                                                                                       >  1:43662/1‑137 (MQ=255)   tCGACCCTGCGCATGGCGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGt                                                                                                                       <  8:335106/137‑1 (MQ=255)       cccTGCGCATGGCGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGGGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTAcgc                                                                                                                   <  6:104107/137‑1 (MQ=255)        ccTGCGCATGGCGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTAcgcg                                                                                                                  <  3:46934/137‑1 (MQ=255)        ccTGCGCATGGCGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTAcgcg                                                                                                                  <  6:116716/137‑1 (MQ=255)         cTGCGCATGGCGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGTTTGGTGGTACgggg                                                                                                                 >  3:226793/1‑137 (MQ=255)             gcATGGCGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGTTTGGGGGTTCGCGGccga                                                                                                             >  3:175441/1‑135 (MQ=255)               aTGGCGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGGTTACTGGTAAGCgggccacg                                                                                                           >  2:332762/1‑131 (MQ=255)                gggCGGAGCGGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGt                                                                                                          <  6:201733/136‑1 (MQ=255)                   cGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGaa                                                                                                       <  5:33412/137‑1 (MQ=255)                    ggAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGTTTGCTTGTTAGCGGGCGAGGTGaaa                                                                                                      >  4:46934/1‑136 (MQ=255)                         tGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGGTTGCTGGGTCGCGGCCGAGGTGTAGCGGTg                                                                                                 >  3:25387/1‑137 (MQ=255)                         tGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGCGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGGTTGCTGGTGAGCCGCCGAGGTGAAGCGGTg                                                                                                 >  7:5827/1‑137 (MQ=255)                           gggCTCGGGGTGGCGCTGGGACGCTCGTCGCTGGGAGAGGAGCTGCTGCAGGAGGGGCGGCTGGTGGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTc                                                                                               <  2:233598/137‑1 (MQ=255)                           gggCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGTTTGCAGGTACGCGGCCGACGGGAAGCGgtgtg                                                                                               >  5:309478/1‑136 (MQ=255)                             gcgcGGGGGGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTccc                                                                                             <  3:314211/134‑1 (MQ=255)                             gCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTccc                                                                                             <  7:102305/137‑1 (MQ=255)                                   ggTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGTTTTCTGGTACGCGGCCGAGGGGCAGCGGTGTgccccccag                                                                                       >  4:304760/1‑133 (MQ=255)                                   ggTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGTTTTCGGGTAAGCGGCCGAGGTGAAGCGTTGTCCCCCGATg                                                                                       >  8:31226/1‑137 (MQ=255)                                   ggTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGTTTGCGGGTTGGCGGCCGAGGGGAAGCGGTGTccccccaag                                                                                       >  3:37483/1‑133 (MQ=255)                                    gTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGTTTGCTGGTTCGCGGGCGAGGTGTAGCGTTGTGCCCCGCTgg                                                                                      >  3:250376/1‑136 (MQ=255)                                                cGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCGAGGGGAAGCGTTGTCCCCCGATGCGCAGGCGTTCAg                                                                          >  8:66593/1‑137 (MQ=255)                                                     gtcgCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCAtt                                                                     >  8:290037/1‑137 (MQ=255)                                                      tcgCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTg                                                                    <  2:188108/137‑1 (MQ=255)                                                         cTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCt                                                                 <  1:242239/137‑1 (MQ=255)                                                          tGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGTTTACTTGTAAGCGGCCTACGTGAAGCGTTGTTCCCCCATGCTCAGGCGTTCAGCCAATGGcgg                                                                >  8:101328/1‑135 (MQ=255)                                                              agagGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCtggtgg                                                            <  2:303288/137‑1 (MQ=255)                                                              agagGACCTGCGGCAGGAGGGGCGGCTGGGCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGGTGTCCCCCGATGCTCAGGCGGTCAGCCATTGGCtggtgg                                                            <  6:239289/137‑1 (MQ=255)                                                                  gACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGTTTTAAGGGTCGCGGCCGAGGGTAAGCGTTTTCCCCCCATGCGCAGGCGTTCAGCCATTGGCgggtgggccg                                                        >  2:79333/1‑133 (MQ=255)                                                                   cccTGCGGCAGGCGGGGCGTCTGGTCGCTCCCGTGGGCCAGCGTATCGCGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGCTGCTCAGGCGGGCAGCCAGTGGCTGGTGGCCATg                                                       <  5:172757/136‑1 (MQ=255)                                                                   aCCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATg                                                       <  1:230713/137‑1 (MQ=255)                                                                   aCCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATg                                                       <  2:201840/137‑1 (MQ=255)                                                                   aCCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATg                                                       <  3:159694/137‑1 (MQ=255)                                                                    ccTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCTAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATgg                                                      <  7:184201/137‑1 (MQ=255)                                                                          gcAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATc                                                <  8:259189/137‑1 (MQ=255)                                                                          gcAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATc                                                <  3:7478/137‑1 (MQ=255)                                                                          gcAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATc                                                <  1:79333/137‑1 (MQ=255)                                                                          gcAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTGTTTACTGGTACGCGGCCTAGGTGAAGCGGTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGGGGGCATGGCGCAtg                                                >  7:258696/1‑136 (MQ=255)                                                                            aggaggGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGt                                              <  8:221933/137‑1 (MQ=255)                                                                                     ggcTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATg                                     <  6:198237/137‑1 (MQ=255)                                                                                     ggcTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATg                                     <  5:260673/137‑1 (MQ=255)                                                                                      gcTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTTGTTGTAGGCGGGCGAAGTGGAGCCGTGTCACCCCCTCCGCAGGCGTTCAGCCAATTTGTGGTGGGCCTGGCGCAGAGGTAACatattt                                    >  4:184811/1‑135 (MQ=255)                                                                                       cTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTa                                   <  8:201105/137‑1 (MQ=255)                                                                                               tCCCTTTGCCCAGAGTATCTAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTg                           <  4:63920/137‑1 (MQ=255)                                                                                                  cTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTGGAg                        <  2:74135/137‑1 (MQ=255)                                                                                                  cTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTGGAg                        <  5:128556/137‑1 (MQ=255)                                                                                                     tGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTGGAGCAc                     <  7:101175/137‑1 (MQ=255)                                                                                                     tGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTGGAGCAc                     <  8:5827/137‑1 (MQ=255)                                                                                                       cccAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTGGAGCACCg                   <  1:335974/137‑1 (MQ=255)                                                                                                       cccAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTGGAGCACCg                   <  5:229230/137‑1 (MQ=255)                                                                                                       cccAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTGGAGCACCg                   <  1:221563/137‑1 (MQ=255)                                                                                                       cacAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCTGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGTCGTTCAGCCATTGGCTGGTGGCCATGGCGCATAGTTAACTTATGTATCTATCTGGAGCACCg                   <  5:25631/135‑1 (MQ=255)                                                                                                            cGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTGGAGCACCGATGCa              <  7:290037/137‑1 (MQ=255)                                                                                                              tATCGAGGCAAGCGAGTCGTGTTTGCTGGGTCGCGGCCGAGGTGAAGCGTTGTCCCCCGATGCGCAGGCGTTAAGCCATTGGGTGGTTGGCATGGCGCATCGGTAACTTATTTAGCTATCTGGAGCACCGATGCAGt            >  6:117218/1‑137 (MQ=255)                                                                                                                      cAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTGGAGCACCGATGCAGTACACATcc    <  4:175441/137‑1 (MQ=255)                                                                                                                        aGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTGGAGCACCGATGCAGTACACATCCAc  <  6:329919/137‑1 (MQ=255)                                                                                                                                  |                                                                                                                               TGTCGACCCTGCGCATGGCGGAGCTGGGGCTCGGGGTGGCGCTGGCACGCTCGTCGCTGGTAGAGGACCTGCTGCAGGAGGGGCGGCTGGTCGCTCCCTTTGCCCAGCGTATCGAGGCAAGCGAGTCGTTTTTCCTGGTACGCGGCCTAGGTGAAGCGTTGTCCCCCGATGCTCAGGCGTTCAGCCATTGGCTGGTGGCCATGGCGCATCGTTAACTTATGTAGCTATCTGGAGCACCGATGCAGTACACATCCAC  >  KT2440/763128‑763383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑