Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,094,8170TG41.5% 16.3 / 10.1 42Q183P (CAG→CCG) CJ019_01995hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/6);  new base G (10/7);  total (29/13)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG  >  KT2440/2094691‑2094936
                                                                                                                              |                                                                                                                       
cGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTg                                                                                                               >  6:247993/1‑137 (MQ=255)
  gTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGGCCCGCGCGCCGGGCCgggcgggc                                                                                                             >  7:76939/1‑137 (MQ=255)
      aGTACCTGGGCATTGGCACTGTAAAAAAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGGGGGccggt                                                                                                         >  3:146513/1‑134 (MQ=255)
             gggCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCCCGCCCGGCCGGGGGGGCCGGTTTGCAgg                                                                                                  >  5:311660/1‑137 (MQ=255)
                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGcc                                                                                               >  5:244480/1‑137 (MQ=255)
                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGGTGGCCGTTTTGGAggggg                                                                                               >  3:75617/1‑135 (MQ=255)
                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCGGGCCGTTTTGCAGGGcc                                                                                               >  7:316835/1‑137 (MQ=255)
                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGGGCGGGCGGGGCTGTTTGGAgggag                                                                                               >  1:82053/1‑135 (MQ=255)
                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGGCCCGGCGGGCCGGTTTGGAggggc                                                                                               >  3:284628/1‑135 (MQ=255)
                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGGCCGGGCGGGGCGTTTTGCAGGGcc                                                                                               >  1:25314/1‑137 (MQ=255)
                      cccGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCGCCGGGCCGGGCGGGCCGTTTTTCACGGCCGGGTgg                                                                                         >  6:187132/1‑137 (MQ=255)
                             cAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGGCCGGGCGGGGCGTTTTTCAGGGCCGGTTGGGCggggg                                                                                  >  8:20453/1‑136 (MQ=255)
                                  ttttCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCGCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCGTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGttgttg                                                                             <  7:285763/136‑1 (MQ=255)
                                  ttttCTCAACATCCGGCCGCTGCTCTCCAAACCCCTTCGGCTCCGGGTGCTTGCGCAAAAACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGttgttg                                                                             <  2:272683/136‑1 (MQ=255)
                                        cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCAGGCTGGCCTTTTTGCAGGGGCTGTTGGCCGAGGTTGTTGAAGAAc                                                                       >  2:217025/1‑137 (MQ=255)
                                                  cAGCTGCTATTCATACCCCTTCGGCTCCGGCTGCTTGCGAAAATACTCCCCGCCGCGCTCCAAGGCCAGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgca                                                             <  6:66881/137‑1 (MQ=255)
                                                     ctgctCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGACGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagca                                                          <  8:295154/137‑1 (MQ=255)
                                                      tgctCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTga                                                                            <  4:280628/118‑1 (MQ=255)
                                                      tgctCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTga                                                                            >  3:280628/1‑118 (MQ=255)
                                                             cATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGACGGCCAGGCGCGCCTGGCCGGGCTGGCCATTTTGCAGGGCCGGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAgg                                                  >  6:63177/1‑137 (MQ=255)
                                                                     ttCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGGTGTTGAAGAACTCTATATGCCTCAGCACCAGGATATGGCg                                          >  4:64096/1‑137 (MQ=255)
                                                                     ttCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCGCCTGGCCGGGGTGGCCTTTTTTCAGGGGCTGTTGGCCGAGGTTGTTGAACTACTCTATATGCATCAGCACCAGGAATTGGCg                                          >  6:186301/1‑137 (MQ=255)
                                                                      tCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCgg                                         >  8:345974/1‑137 (MQ=255)
                                                                      tCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCgg                                         >  8:270714/1‑137 (MQ=255)
                                                                        ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCGGGGCTTTTTTCAGGGGCGGTTGGCCGAGGGTGTTGTAGAACTATATATGGAGCAGCACCAGGATATTGCGGAt                                       >  3:83422/1‑137 (MQ=255)
                                                                        ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGGCCGGGCTGGCCTTTTTTCAGGGCCGCTTGGGCGAGGTTGTTGAAGAACTCAATATGCAGCAGCACCAGGATATGGCGGAt                                       >  5:222224/1‑137 (MQ=255)
                                                                         gCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCCGGATTTGGCGGAtt                                      >  4:60239/1‑137 (MQ=255)
                                                                          cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGGCTTTTTGCAGGGCGTGGTGGCCGAGGTTGTTGCAGCACTCTATATGCAGCAGCACCAGGATATGggggttgt                                     >  3:25908/1‑133 (MQ=255)
                                                                          cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAgtt                                     >  7:23199/1‑137 (MQ=255)
                                                                                 tgctTGCGCAAATACTGCACGCCGCGCTCGAAGGCCATGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCGGGTGGCCGAGGTTGTTGAAGCACTCTATATGCATCAGCCCCAGGATATGGCGGATGTCCTTGAc                              >  8:288198/1‑137 (MQ=255)
                                                                                     tGCGCAAATACTCCACGCCGCGCGCTAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                          <  2:82053/137‑1 (MQ=255)
                                                                                                  ccgcccgcGCGCGCGGGCCCGGCGCGCGGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGa             <  5:187132/133‑1 (MQ=255)
                                                                                                  cACGCGGCGCTCGACGGCCAGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGa             <  6:311660/137‑1 (MQ=255)
                                                                                                    cgccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa           >  7:63873/1‑137 (MQ=255)
                                                                                                    cgccgcGCGCGATGGCCTGGCGCGCCGGCCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa           <  7:274399/137‑1 (MQ=255)
                                                                                                       cgcgCTCGACGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCATGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCg        <  8:76939/137‑1 (MQ=255)
                                                                                                       cgcgCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCCGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCg        >  4:76011/1‑137 (MQ=255)
                                                                                                         cgcTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGGCGAGGTTGTTGAAGAAATCTATATTCAGCAGCACCAGGATATGGCGGATTTCCTTGAACCAATGCTTGGCCTCGACAGCggg      >  8:100349/1‑137 (MQ=255)
                                                                                                          gcTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCGTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGc     >  8:284658/1‑137 (MQ=255)
                                                                                                             cgttggCGAGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  7:181340/133‑1 (MQ=255)
                                                                                                             cGACGGCCTGGCGCGCCGGCCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  7:94149/137‑1 (MQ=255)
                                                                                                             cGACGGCCCGGCGCGGCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  3:160664/137‑1 (MQ=255)
                                                                                                                              |                                                                                                                       
CGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG  >  KT2440/2094691‑2094936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑