Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,245,145	0	A	C	38.0%	35.2 / 12.1	53	L178R (CTG→CGG)	CJ019_03982	Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (10/21);  new base C (16/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 4.50e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCG  >  KT2440/4245010‑4245270                                                                                                                                        |                                                                                                                              cGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCac                                                                                                                              >  6:116653/1‑136 (MQ=255)  gggCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCCACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCgg                                                                                                                             >  2:297453/1‑137 (MQ=255)   ggCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCAGCGCAGACTTCGCTGACCAACCCGGCGGCCCggg                                                                                                                            >  2:71943/1‑137 (MQ=255)         gTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCTAAGTGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGAGTTCGCTGACCAACCCGGCGGCCAGGGCGCGAt                                                                                                                      <  5:340000/137‑1 (MQ=255)                  cAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGcccc                                                                                                             >  7:301933/1‑137 (MQ=255)                     ccGCTTGGTCTGCGGATTGGCGAAGGGCGGGCCATTGGCAAGGCGCCCGGCCTGTGCGGCGGCAACGGAAGGCAACTGGGCGTCGGGGCAGACTTGGCTGACCAACCCGGCGGCATGGGCGCGATCGGCGCCCcaca                                                                                                          <  6:323342/137‑1 (MQ=255)                       gCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAAc                                                                                                        >  3:264319/1‑137 (MQ=255)                           ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCACCACtcgt                                                                                                    >  1:99737/1‑137 (MQ=255)                             tCTGCGCATAGGCGAAGGTCGGGCCATTGGTCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACtcgtcg                                                                                                  >  4:36065/1‑137 (MQ=255)                             tCTGCGCATAGGCGAAGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACtcgtcg                                                                                                  <  6:84941/137‑1 (MQ=255)                             gcTGCGCATAGGCTATGGTCGGGCCATTGGCCAGGCGCCCGGCCTGTTCGGCGGCAACGGCAGGCAACTGCGCGTCGGCGCAGACTTCGCTCACCAACCCGGCGGCCTGGGCGCTATCGGCGCCCCACAACtcgtcg                                                                                                  <  4:45195/136‑1 (MQ=255)                             gcTGCGCATAGGCGAAGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACtcgtcg                                                                                                  <  3:98455/136‑1 (MQ=255)                                  gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACTCGTCGCGGaa                                                                                             >  3:333146/1‑137 (MQ=255)                                    aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCGGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                           <  4:144070/137‑1 (MQ=255)                                    aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCACACCTCGTCGAGGcaca                                                                                           >  7:282918/1‑137 (MQ=255)                                    aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAAcc                                                                                           >  4:184201/1‑136 (MQ=255)                                         cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACGCGGCGAGGAACACCAgg                                                                                      >  1:308734/1‑137 (MQ=255)                                         cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACACCTCGGCGAGGAACAGCCgg                                                                                      >  6:203220/1‑137 (MQ=255)                                         cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCCgg                                                                                      >  1:66258/1‑137 (MQ=255)                                         cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACGTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGGGGAGGAACAGCAgg                                                                                      >  7:261713/1‑137 (MQ=255)                                            cgggcGGGCCATTGGCCAGGCGCCGGGCCAGTTCGGCGGCACCGGCAGGCAAGTGCTCGGCGGCGCAGACTTCGCGGACCAACCCGGCGGCCAGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGgcgc                                                                                   <  6:48535/133‑1 (MQ=255)                                              ggcGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCGCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCtt                                                                                 <  3:303859/135‑1 (MQ=255)                                              gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCtt                                                                                 >  3:271304/1‑137 (MQ=255)                                                      aagggCCAGGCGCCCGGCCATGTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCt                                                                         <  8:264171/134‑1 (MQ=255)                                                             cggCGCCCGGCCAGTTCGGCGGCAACGGCAGGAAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                  <  6:159187/136‑1 (MQ=255)                                                             cggCGCCCGGCCAGTGCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTGCCCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                  <  8:187007/136‑1 (MQ=255)                                                                cgcCCAGCCAGTTCGTCGGAAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCACGCCCTTGGCCGGGTCGCTGCCGAt                                                               >  5:292848/1‑137 (MQ=255)                                                                  cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATAGGCGCCCCCCAACTCGGCGAGGCACCGCCCGCGCTTGGCCGGCTCGCTGCCGAtac                                                             >  3:270589/1‑135 (MQ=255)                                                                       ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACCACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCgg                                                        >  5:338704/1‑136 (MQ=255)                                                                                        gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCCACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                       >  4:149630/1‑137 (MQ=255)                                                                                        gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGCACAGCAGGCGCTTGGCCTGCTCGCTGCCGCTCAGCCGTGGCAGGTGCCAGCTGgc                                       >  3:143833/1‑137 (MQ=255)                                                                                        gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                       >  5:358327/1‑137 (MQ=255)                                                                                        gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                       <  8:301933/137‑1 (MQ=255)                                                                                               ccTGCGCGGCGGCGCAGACTTCGCTGACCACCCCGGCGGCCAGGGCGCTACCGGCGCCCCACAACTCGTCGAGGACCAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                <  4:271953/136‑1 (MQ=255)                                                                                               agtGCTCGCCGGCGCTGCGTCCGCTGCCCACCCCGCCGCCCCGGGCGCGAGCGGCGCCCCACAACTCGGCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                <  8:300892/135‑1 (MQ=255)                                                                                               aCTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                <  6:358327/137‑1 (MQ=255)                                                                                                    tcgtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                           >  7:305822/1‑137 (MQ=255)                                                                                                    gcgtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                           <  4:264319/136‑1 (MQ=255)                                                                                                       gcgGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCt                        <  4:263372/136‑1 (MQ=255)                                                                                                         ggCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGTGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                      >  4:296911/1‑137 (MQ=255)                                                                                                            gcAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGc                   <  5:131106/137‑1 (MQ=255)                                                                                                              ataCTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCATGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGTGGCTGTAGccc                 <  4:324496/135‑1 (MQ=255)                                                                                                              aGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc                 <  3:44141/137‑1 (MQ=255)                                                                                                                     gCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCCGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTgg          >  2:88334/1‑137 (MQ=255)                                                                                                                     gCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTgg          >  2:115854/1‑137 (MQ=255)                                                                                                                        gACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt       <  6:67489/137‑1 (MQ=255)                                                                                                                        gACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAAATCCTCGAGGAACAGCAGGCGCTTTGCCAGCTCGCTGCCGATCAGCCGTGGCAGGTTCCAGCTGGCGCCCGCATCGGGGCGGTAGCCCATGCTGgtgt       >  4:198009/1‑137 (MQ=255)                                                                                                                        gACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt       <  4:182718/137‑1 (MQ=255)                                                                                                                        gACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt       <  3:149630/137‑1 (MQ=255)                                                                                                                        caccaccCGGGCGGCCGGGGGGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt       <  1:335080/136‑1 (MQ=255)                                                                                                                        caccaccCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt       <  3:198009/136‑1 (MQ=255)                                                                                                                        caccaccCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt       <  6:292848/136‑1 (MQ=255)                                                                                                                        caCCAACCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt       <  1:71943/136‑1 (MQ=255)                                                                                                                             aCCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCg  >  6:288682/1‑137 (MQ=255)                                                                                                                                        |                                                                                                                              CGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCG  >  KT2440/4245010‑4245270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑