| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 690,144 | A→G | intergenic (+21/‑2438) | bauC → / → PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 690,144 | 0 | A | G | 91.7% | 14.2 / ‑6.1 | 13 | intergenic (+21/‑2438) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (11/0); minor base T (1/0); total (13/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGG‑CC‑GTCAGGC‑CTCCCCG‑C‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGA > KT2440/690043‑690210 | aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGG‑CCGGGCCGGCGCCCCCCG‑G‑TTT‑‑TTTTTGGGggt > 2:27505/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGG‑CG‑GGCCGGC‑C‑CCCCG‑CGGTGG‑TTTTTTGGggtgggtttt > 5:290848/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCC‑GGCTGGC‑GACCCCC‑C‑TGGT‑TTTTTGGGGGGTGgttgt > 8:38019/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGG‑GC‑GTCCGGG‑CGCCCCC‑C‑GTTT‑TTTTTTGGGGGTGgttgtt > 2:236022/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGG‑GC‑GGGAGGG‑CGGCCCC‑C‑CGTG‑TTTTTTTGGGGTGgttgtt > 5:327796/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGG‑GC‑GGGAGGG‑CGCCCCC‑C‑GTTT‑TTTTTTGGGGTTTgttgtt > 2:66783/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGG‑GC‑GGGAGGC‑CGCCCCC‑C‑GTTG‑TTTTTTGGgggtggtgtgt > 7:73591/1‑133 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGG‑CC‑GACCCGC‑CGCCCCC‑C‑GTTG‑TTTTTTGGGGGTGggtgtt > 6:258639/1‑137 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGTGG‑GC‑CGGAGGC‑CGGCCCGCC‑GTTG‑TTTTTGGGGGTGggtggtgg > 7:294139/1‑135 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGG‑CC‑GGCAGGG‑CGCCCCC‑C‑GTTG‑TTTTTTGGGGGTGggtgtttg > 6:300376/1‑135 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCGG‑GG‑CTGAGGG‑CGCCCCC‑C‑GGTGTTTTTTTGGggttgggtttgg > 7:38721/1‑131 (MQ=255) ttGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGG‑GC‑GGCGCGC‑CCCCCCC‑C‑GGTGTTTTTTGTGGGTGGGTTGTTGGTGTTGGgggggactgg > 5:96899/1‑133 (MQ=255) cGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGG‑CG‑GTCGGGC‑GGCCCGG‑G‑GTGG‑TTTTGGGGGGGGGTTTGTTG‑GGTTGGGGGTGAGCTGtttttt > 5:337962/1‑135 (MQ=255) | ACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGG‑CC‑GTCAGGC‑CTCCCCG‑C‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGA > KT2440/690043‑690210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |