Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 3,061,793 G→A S592N (AGC→AAC)  qedA_2 → Quinoprotein alcohol dehydrogenase (cytochromec)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,061,7930GA96.9% 97.7 / NA 32S592N (AGC→AAC) qedA_2Quinoprotein alcohol dehydrogenase (cytochromec)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (22/9);  minor base C (0/1);  total (22/10)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGC  >  KT2440/3061659‑3061926
                                                                                                                                      |                                                                                                                                      
ggTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCTGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                      <  4:255891/136‑1 (MQ=255)
cGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                      <  6:208327/137‑1 (MQ=255)
cGTTTCGGTAGTCTCCGGCGGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAATCGGGTCAAGGACTTCAACCAGGGCGCCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                      <  5:11866/137‑1 (MQ=255)
      ggTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATTCTCTGGACCATCAACTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGc                                                                                                                                >  1:297808/1‑137 (MQ=255)
       gTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGcc                                                                                                                               >  2:300860/1‑137 (MQ=255)
        tAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCa                                                                                                                              >  8:283787/1‑137 (MQ=255)
             tcCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGTCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACg                                                                                                                         <  2:103205/137‑1 (MQ=255)
               cGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACgc‑c                                                                                                                      <  6:337187/137‑1 (MQ=255)
                   tGGGGCGGCGCGGTGGCGCTGTGGGGCGGCGAAGTGGCCAAACTGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACgc‑ctgcc                                                                                                                  <  3:146083/137‑1 (MQ=255)
                       gcggcgCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACt                                                                                                              >  7:78360/1‑137 (MQ=255)
                                  ccGCTGTGGGGCGGCGAAGTGGCCAATCGGGTCAAGGACTTCAAGCAGGGCGGCATGCTCTGGACCTTCAAGTTCCCCATGCTGTTGCAGCATACGGGAAACGTCAAGCCTTATCTC‑CTGCCTACTGCCGCTtgctg                                                                                                   <  4:324762/137‑1 (MQ=255)
                                    gCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCCGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGcc                                                                                                 >  6:251748/1‑137 (MQ=255)
                                       gtgGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCt                                                                                              <  1:300860/137‑1 (MQ=255)
                                             cggcgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCgg                                                                                        >  6:261290/1‑137 (MQ=255)
                                                          aaaCGGGTCAAGGACTTCACCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGGAAGCGGccc                                                                           >  8:231474/1‑137 (MQ=255)
                                                                   aaGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCAGCTGATCGGTGGCAAGCGGCCCTACTACCaa                                                                  >  6:88838/1‑137 (MQ=255)
                                                                    aGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCaaa                                                                 >  5:25902/1‑137 (MQ=255)
                                                                            aaCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACgaga                                                         <  4:203889/137‑1 (MQ=255)
                                                                             aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGagag                                                        >  3:318144/1‑137 (MQ=255)
                                                                             aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGagag                                                        >  1:74460/1‑137 (MQ=255)
                                                                              ccAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGc                                                       >  7:235554/1‑137 (MQ=255)
                                                                                    cggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGcg                                                 >  5:198093/1‑136 (MQ=255)
                                                                                    cggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCt                                                 >  4:87257/1‑137 (MQ=255)
                                                                                      gcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATTACGAGCGCCCCGCTGc                                               >  7:298761/1‑137 (MQ=255)
                                                                                      gaaTGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCTGCAAACCGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTCCTGCCGCTGCTCGGACCCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCAGc                                               >  3:156100/3‑137 (MQ=255)
                                                                                                   cTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTc                                  >  5:106083/1‑137 (MQ=255)
                                                                                                   cTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTc                                  >  4:93642/1‑137 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCCAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCg                             >  4:300801/1‑137 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCg                             >  7:355683/1‑137 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCg                             >  4:31284/1‑137 (MQ=255)
                                                                                                               caacccgTTGCAGCAACCGGCAAACGTAAACCAAAAACGC‑CTGCCTCCTGCCGCGGGCGGCCGGTTATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcgcg                      <  6:3537/131‑1 (MQ=255)
                                                                                                                    aGTTGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CGGCCTACTGCCGCTGGCGGCCGCTGAGCGGGGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGct                 <  5:342300/137‑1 (MQ=255)
                                                                                                                       tGCAGCAAACGGCAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGcttct              <  3:93642/137‑1 (MQ=255)
                                                                                                                          agcaAACGCAAACCGTCAA‑CCAAAACCCACTTCCTCCTCCCGCTTGGTGCCGCTCATCGGGGGCAGGCGCCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAc           <  2:141057/137‑1 (MQ=255)
                                                                                                                           gcaaacggcaaacgTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAcc          >  3:212003/1‑137 (MQ=255)
                                                                                                                                   cAAACGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGc  >  4:248812/1‑137 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGC‑CTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGC  >  KT2440/3061659‑3061926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑