Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 3,957,395 C→G G85A (GGG→GCG)  dmlR_13 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,957,3950CG100.0% 182.3 / NA 52G85A (GGG→GCG) dmlR_13HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (26/26);  total (26/26)

GCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGGGTGGTGCGGTGCAGCAGGCGGGTCTGCAGCTGGTT  >  KT2440/3957264‑3957525
                                                                                                                                   |                                                                                                                                  
gcTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTcgc                                                                                                                               <  8:349637/137‑1 (MQ=255)
gcTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTcgc                                                                                                                               >  3:158728/1‑137 (MQ=255)
     gACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTc                                                                                                                          <  5:170721/137‑1 (MQ=255)
       cAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCGAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGc                                                                                                                        >  7:159360/1‑137 (MQ=255)
             cAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACgg                                                                                                                  >  4:339848/1‑137 (MQ=255)
             cAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACgg                                                                                                                  >  8:328129/1‑137 (MQ=255)
               gCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGct                                                                                                                <  3:19728/137‑1 (MQ=255)
                       accacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCACGCCTCGCCCACGGCTTCTACGGt                                                                                                        >  5:278605/1‑137 (MQ=255)
                           cTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTcagc                                                                                                    <  1:176539/137‑1 (MQ=255)
                                gggTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCAt                                                                                               <  7:163632/137‑1 (MQ=255)
                                          tgttgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgc                                                                                     <  7:328129/137‑1 (MQ=255)
                                             tgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATGTGCCTCGCa                                                                                  <  8:107415/137‑1 (MQ=255)
                                              gtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCAc                                                                                 >  2:280411/1‑137 (MQ=255)
                                              gtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCAAGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCAc                                                                                 >  8:345589/1‑137 (MQ=255)
                                              gtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGAGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTATTCGGTCAGCAGCCTGATCTGCCGAGCAc                                                                                 >  8:340716/1‑137 (MQ=255)
                                                    ggCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCa                                                                           >  7:169585/1‑137 (MQ=255)
                                                          caGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAAcc                                                                     >  8:317586/1‑137 (MQ=255)
                                                          caGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAAcc                                                                     <  8:129550/137‑1 (MQ=255)
                                                            gCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGc                                                                   <  5:296419/137‑1 (MQ=255)
                                                                         cAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGc                                                      <  4:3282/137‑1 (MQ=255)
                                                                            ccccATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTca                                                                                                      <  3:54925/86‑1 (MQ=255)
                                                                            ccccATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTca                                                                                                      >  4:54925/1‑86 (MQ=255)
                                                                             cccATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCAt                                                  <  8:354867/137‑1 (MQ=255)
                                                                             cccATGCCGGGCGGGCTGTGCTAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGACCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCCCAt                                                  <  1:260059/137‑1 (MQ=255)
                                                                                 tGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATgcgc                                              >  7:26090/1‑137 (MQ=255)
                                                                                   ccGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgcg                                                                                    >  5:289671/1‑97 (MQ=255)
                                                                                   ccGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgcg                                                                                    <  6:289671/97‑1 (MQ=255)
                                                                                   ccGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAg                                            <  7:189806/137‑1 (MQ=255)
                                                                                   ccGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAg                                            <  3:20648/137‑1 (MQ=255)
                                                                                   ccGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAg                                            <  7:320715/137‑1 (MQ=255)
                                                                                    cGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAgg                                           >  2:99691/1‑137 (MQ=255)
                                                                                     gTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGgc                                          <  1:40845/137‑1 (MQ=255)
                                                                                         gggCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTc                                                        <  5:13824/119‑1 (MQ=255)
                                                                                         gggCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTc                                                        >  6:13824/1‑119 (MQ=255)
                                                                                         gggCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCg                                      >  6:282704/1‑137 (MQ=255)
                                                                                         gggCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCg                                      >  6:275176/1‑137 (MQ=255)
                                                                                         gggCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCg                                      >  5:339429/1‑137 (MQ=255)
                                                                                         gggCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCg                                      >  1:78669/1‑137 (MQ=255)
                                                                                         gggCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCg                                      >  7:86700/1‑137 (MQ=255)
                                                                                         gggCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTGCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCg                                      >  3:33281/1‑137 (MQ=255)
                                                                                         gggCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCGTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCg                                      >  8:304507/1‑137 (MQ=255)
                                                                                             aGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGggtg                                  <  4:279632/137‑1 (MQ=255)
                                                                                                 cAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGGGTggtgc                              >  4:217607/1‑137 (MQ=255)
                                                                                                  aaaCGCAGATGGCCGCGAGGCGTACGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGGGTggtgcg                             <  6:98633/137‑1 (MQ=255)
                                                                                                  aaaCGCAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGGGTggtgcg                             <  8:102789/137‑1 (MQ=255)
                                                                                                       cAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGGGTggtgcg                             <  7:324942/132‑1 (MQ=255)
                                                                                                       cAGATGGCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGGGTggtgcg                             >  8:324942/1‑132 (MQ=255)
                                                                                                            ggCCGCGAGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGGGTGGTGCGGTGCAGCAgg                   >  3:149323/1‑137 (MQ=255)
                                                                                                                   aGGCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGGGTGGTGCGGTGCAGCAGGCGGGTCt            >  2:126456/1‑137 (MQ=255)
                                                                                                                     gCGTAAGGTGCGCCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGGGTGGTGCGGTGCAGCAGGCGGGTCTGc          <  4:158728/137‑1 (MQ=255)
                                                                                                                      cGTAAGGTGCGCCGCTCGCGCCGCGCCCACGGCTTCTTCGGTCAGCAGCATCATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGGGTGGTGCGGTGCAGCAGGCGGGTCTGCa         <  5:20304/137‑1 (MQ=255)
                                                                                                                             ggcgcCGCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGAATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGGGTGGTGCGGTGCAGCAGGCGGGTCTGCAGCTGGtt  <  4:216084/136‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                  
GCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGCGCCGGGTGGTGCGGTGCAGCAGGCGGGTCTGCAGCTGGTT  >  KT2440/3957264‑3957525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: