Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,846,725 Δ1 bp 5.5% coding (847/1056 nt) CJ019_05475 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,846,7250C. 5.5% 247.5 / 7.8 73coding (847/1056 nt)CJ019_05475hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (38/31);  new base . (2/2);  total (40/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

CGCCGCATTCTTGGCAATCATCCCGTTGAGCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTCCAGGCCCTCGTTAGCCTTGGCATCGTAGT  >  KT2440/5846591‑5846856
                                                                                                                                      |                                                                                                                                   
cgccgcATTCTTGGCAATCATCCCGTTGAGCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTcgcc                                                                                                                                     >  3:199782/1‑135 (MQ=255)
cgccgcATTCTTGGCAATCATCCCGTTGAGCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGCACCTGCAGGAAGCCATCGCTGGTCGGCGTGTcgcc                                                                                                                                     >  5:75731/1‑135 (MQ=255)
              cAATCATCCCGTTGAGCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCTCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCAcc                                                                                                                        <  4:185762/134‑1 (MQ=255)
              cAATCATCCCGTTGAGCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCAcc                                                                                                                        <  1:143616/134‑1 (MQ=255)
              cAATCATCCCGTTGAGCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCAcc                                                                                                                        <  4:199782/134‑1 (MQ=255)
                    tcCCGTTGAGCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTttc                                                                                                                  <  6:287210/134‑1 (MQ=255)
                       cGTTGAGCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCACCACCGGTttcttac                                                                                                              >  8:215271/1‑133 (MQ=255)
                       cGTTGAGCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCACACCAGCACCGGTTTCTTcc                                                                                                              >  2:264457/1‑135 (MQ=255)
                       cGTTGAGCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCG‑‑GCCCACCAGCACCGGTTTCTTCCAc                                                                                                            >  4:326101/1‑135 (MQ=255)
                          tGAGCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACt                                                                                                           <  8:32586/135‑1 (MQ=255)
                          tGAGCTGGGCCATGTACTTGGCCGTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACt                                                                                                           <  1:259938/135‑1 (MQ=255)
                             gCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCAt                                                                                                        <  8:37638/135‑1 (MQ=255)
                               tGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCg                                                                                                      <  5:34165/135‑1 (MQ=255)
                               tGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCg                                                                                                      <  7:64122/135‑1 (MQ=255)
                                    cATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCCCCGGTTTCTTGCACTCATAGATGTa                                                                                                 >  7:110784/1‑135 (MQ=255)
                                    cATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTa                                                                                                 >  5:121205/1‑135 (MQ=255)
                                    cATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTa                                                                                                 >  1:250509/1‑135 (MQ=255)
                                         aCTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTaa                                                                                            >  5:121568/1‑135 (MQ=255)
                                              gCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATc                                                                                       >  3:177302/1‑135 (MQ=255)
                                                   gCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATcgccgc                                                                                  >  6:142296/1‑135 (MQ=255)
                                                   gCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATcgccgc                                                                                  >  4:103733/1‑135 (MQ=255)
                                                     ggTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCCCCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGCTCGCCGCCt                                                                                >  1:9245/1‑135 (MQ=255)
                                                      gTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCCCCCGCCCCGGTTTCGTGCACGCATCGGAGTAGGTAAGGATCgccgccgg                                                                               >  1:326762/1‑133 (MQ=255)
                                                      gTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCGTCCCCTCATCGATGTAGGTAAGGATCGCCGCCTg                                                                               >  1:6833/1‑135 (MQ=255)
                                                          aTCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCtt                                                                            <  6:121568/134‑1 (MQ=255)
                                                            ccACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGCACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTGGCCCTCATCGAGGTAGGTAAGGATCGCCGCCTGCTTGcc                                                                         >  1:267199/1‑135 (MQ=255)
                                                            ccACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGGTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGcc                                                                         >  6:102953/1‑135 (MQ=255)
                                                                        aCCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCCCCCGCACCGGGTTCTTCCACTCATCGATGTAGGTAAGGCTCGCCGCCTGCTTGCCCGCCCTCCCGGt                                                             >  5:253204/1‑135 (MQ=255)
                                                                        aCCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGt                                                             >  4:159386/1‑135 (MQ=255)
                                                                          cggcTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCCTCGCTGGTCGGCGGGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTgg                                                           <  4:323/132‑1 (MQ=255)
                                                                          cGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTgg                                                           <  8:50987/135‑1 (MQ=255)
                                                                          cGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTgg                                                           <  3:235248/135‑1 (MQ=255)
                                                                          cGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTgg                                                           <  2:250509/135‑1 (MQ=255)
                                                                             cTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCg                                                        >  4:323852/1‑135 (MQ=255)
                                                                             cTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCG‑‑GCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCggg                                                      <  3:326101/135‑1 (MQ=255)
                                                                               ttGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCggg                                                      >  6:83469/1‑135 (MQ=255)
                                                                               ttGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCggg                                                      >  7:197924/1‑135 (MQ=255)
                                                                                 gCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCggg                                                      <  5:209779/133‑1 (MQ=255)
                                                                                  cccACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGt                                                    <  4:77503/134‑1 (MQ=255)
                                                                                  cccACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGt                                                    <  1:253319/134‑1 (MQ=255)
                                                                                  cccACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGt                                                    <  4:177302/134‑1 (MQ=255)
                                                                                  cccACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGGCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGt                                                    <  8:181448/134‑1 (MQ=255)
                                                                                   ccACCTCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTcc                                                  <  2:17472/135‑1 (MQ=255)
                                                                                          cACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAgg                                             >  5:319516/1‑133 (MQ=255)
                                                                                          cACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGC‑‑CCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCggg                                                      <  7:185206/122‑1 (MQ=255)
                                                                                          cACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGC‑‑CCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCggg                                                      >  8:185206/1‑122 (MQ=255)
                                                                                          cACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGACGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAgg                                             >  8:63503/1‑133 (MQ=255)
                                                                                             cccGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAgg                                        <  6:121205/135‑1 (MQ=255)
                                                                                             cccGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAgg                                        <  6:191581/135‑1 (MQ=255)
                                                                                               cGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAgggg                                      >  7:213303/1‑135 (MQ=255)
                                                                                               cGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAgggg                                      >  8:7810/1‑135 (MQ=255)
                                                                                                    aaCTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTcc                                                                                                              >  7:84607/1‑58 (MQ=255)
                                                                                                    aaCTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTcc                                                                                                              <  8:84607/58‑1 (MQ=255)
                                                                                                    aaCTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGCTCGCCGCCTGCTTGCCCGCCATCCCGGTGGCCGGGGTCCACAGGTAAGGGGTCCGc                                 >  5:21562/1‑135 (MQ=255)
                                                                                                    aaCTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAgg                                        >  1:56552/1‑128 (MQ=255)
                                                                                                    aaCTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAgg                                        <  2:56552/128‑1 (MQ=255)
                                                                                                    aaCTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGc                                 >  8:260557/1‑135 (MQ=255)
                                                                                                    aaCTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGc                                 >  2:207443/1‑135 (MQ=255)
                                                                                                    aaCTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCACCACCGGTTTCTTCCCCTCATAGATGGATGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCCCAGGTAAGGGGTCAGc                                 >  2:100805/1‑135 (MQ=255)
                                                                                                    aaCTGCAGGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCCCCATCACCGGTGTCTTCCCCTCATCGATGTAGGTAAGGATCGCCGACTGCTTGCCCGCCATCCAGTTGGCAGGGGGCCACAGGTAAGGGGTCAGc                                 >  2:279899/1‑135 (MQ=255)
                                                                                                      cTGCATGTAGCCATCGCTGGGCGGCGTGGCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTc                               <  6:201205/135‑1 (MQ=255)
                                                                                                       tGCATGTAGCCATCGCTGGTCGGCGTGTCGACGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTcc                              <  7:63503/135‑1 (MQ=255)
                                                                                                          aTGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAgg                                        >  1:219446/1‑122 (MQ=255)
                                                                                                          aTGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAgg                                        <  2:219446/122‑1 (MQ=255)
                                                                                                            gTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACt                                                                                                           >  3:256841/1‑53 (MQ=255)
                                                                                                            gTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACt                                                                                                           <  4:256841/53‑1 (MQ=255)
                                                                                                            gTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTCCCGGcc                         >  7:16059/1‑135 (MQ=255)
                                                                                                                ccATCGATGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTCCAGGCCCTc                      <  5:29342/134‑1 (MQ=255)
                                                                                                                     gCTGGGCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGGCAGCTCCAGGCCCTCGTTAg                 <  5:27624/134‑1 (MQ=255)
                                                                                                                      cTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCCCAGGTAAGGGGTCAGCTCCAGGCCCTCGTTAGcc               >  3:222541/1‑135 (MQ=255)
                                                                                                                      cTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTCCAGGCCCTCGTTAGcc               >  7:196945/1‑135 (MQ=255)
                                                                                                                      cTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTCCAGGCCCTCGTTAGcc               >  2:64045/1‑135 (MQ=255)
                                                                                                                      cTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTCCAGGCCCGCGTTAGcc               >  6:249741/1‑135 (MQ=255)
                                                                                                                      cTGGTCGGCGTGTCGCCGCCCACCAGCACCGGGTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTCCAGGCCCTCGTTAGcc               >  1:122187/1‑135 (MQ=255)
                                                                                                                       tGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTCCAGGCCCTCGTTAGCCt              <  6:319516/135‑1 (MQ=255)
                                                                                                                                   cgccgcCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTCCAGGCCCTCGTTAGCCTTGGCATCGTAGt  <  7:7810/135‑1 (MQ=255)
                                                                                                                                   cgccgcCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTCCAGGCCCTCGTTAGCCTTGGCATCGTAGt  <  6:212072/135‑1 (MQ=255)
                                                                                                                                   cgccgcCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTCCAGGCCCTCGTTAGCCTTGGCATCGTAGt  <  4:260738/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
CGCCGCATTCTTGGCAATCATCCCGTTGAGCTGGTCCATGTACTTGGCCTTGCGGTTTATCCACAGGTGGATACCGCCTTTGCCCACATCCACCCCGTGGAACTGCATGTAGCCATCGCTGGTCGGCGTGTCGCCGCCCACCAGCACCGGTTTCTTCCACTCATCGATGTAGGTAAGGATCGCCGCCTGCTTGCCCGCCATCCAGGTGGCCGGGGTCCACAGGTAAGGGGTCAGCTCCAGGCCCTCGTTAGCCTTGGCATCGTAGT  >  KT2440/5846591‑5846856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑