Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	5,426,158	Δ1 bp	5.8%	coding (186/519 nt)	CJ019_05104 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,426,158	0	T	.	5.8%	234.8 / 7.7	69	coding (186/519 nt)	CJ019_05104	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (40/25);  new base . (2/2);  total (42/27)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

CACCGTCGACCGGGACATCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTACTGGCATGCAGAGGTTAGCGAATACTGGA  >  KT2440/5426032‑5426291                                                                                                                               |                                                                                                                                      cACCGTCGACCGGGACATCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTc                                                                                                                               >  2:210996/1‑135 (MQ=255)  aCCGTCGACCGGGACATCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTcg                                                                                                                              >  3:222715/1‑135 (MQ=255)   ccGTCGACCGGGACATCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTcgc                                                                                                                             >  4:171109/1‑135 (MQ=255)    tgTCGACCGGGACATCCCGCTACGCCTCAGCCGCAGTACAGCCAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGGGATGGACTTTCATTACCACTcgc                                                                                                                             >  8:198991/2‑134 (MQ=255)    cGTCGACCGGGACATCCCGCTACGCCTCAGCCGCAGTACCGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTcgcg                                                                                                                            >  1:276210/1‑135 (MQ=255)    cGTCGACCGGGACATCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTcgcg                                                                                                                            >  3:308904/1‑135 (MQ=255)    cGTCGACCGGGACATCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTcgc                                                                                                                             >  5:148982/1‑134 (MQ=255)     gTCGACCGGGACATCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCgg                                                                                                                           >  3:170466/1‑135 (MQ=255)      tCGACCGGGACATCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGa                                                                                                                          >  8:275329/1‑135 (MQ=255)      tCGACCGGGACATCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGa                                                                                                                          >  2:145711/1‑135 (MQ=255)         aCCGGGACATCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCa                                                                                                                       >  8:53619/1‑135 (MQ=255)            gggACATCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACg                                                                                                                    <  1:210996/135‑1 (MQ=255)            gggACATCCCGCTACGCATCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACg                                                                                                                    >  8:16171/1‑135 (MQ=255)                 aTCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGc                                                                                                               >  1:43125/1‑135 (MQ=255)                 aTCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGc                                                                                                               <  6:94569/135‑1 (MQ=255)                   cccGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCt                                                                                                             <  7:275329/135‑1 (MQ=255)                   cccGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGGGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCt                                                                                                             <  8:275480/135‑1 (MQ=255)                      gCTAGGCGTCTGCGGCACTACAGACAAGTGCCTCTGGGGCATTACCCATGAGGATGAGTGGTTGCAGTCGGGTGTACAGAAGTACTCGCCGGTTATGGACTTTCATTACAACTCGCGGACCAACGATCGCCTGCa                                                                                                          <  6:257536/135‑1 (MQ=255)                           gCCTCAGCCGGAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCTGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCTCGGACCAACGATCGCCTGCACTAcc                                                                                                     >  4:25614/1‑135 (MQ=255)                            ccTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGACAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCg                                                                                                    <  7:244695/135‑1 (MQ=255)                                aGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCa                                                                                                  >  6:65437/1‑133 (MQ=255)                                  ccGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCa                                                                                               >  6:221346/1‑134 (MQ=255)                                     cAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCAt                                                                                           <  2:210653/135‑1 (MQ=255)                                         aCAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCa                                                                                       <  7:325681/135‑1 (MQ=255)                                          cAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAg                                                                                      >  7:318540/1‑135 (MQ=255)                                          cAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCA‑TACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAgg                                                                                     >  8:307799/1‑135 (MQ=255)                                                aGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGcc                                                                                >  4:150815/1‑135 (MQ=255)                                                aGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCA‑TACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGccc                                                                               >  6:145712/1‑135 (MQ=255)                                                     ggCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGgag                                                                           >  5:80533/1‑135 (MQ=255)                                                     ggCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGgag                                                                           >  8:6578/1‑135 (MQ=255)                                                     ggCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATAACCACTCGCGGACCACCGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGgag                                                                           >  5:235070/1‑135 (MQ=255)                                                     ggCTGGGGCATTACACCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGCCTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGgag                                                                           >  2:298663/1‑135 (MQ=255)                                                       cTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTTCACTACCTCATCAGCATGCCAGGGCGCCCGGAGAc                                                                         <  8:65594/135‑1 (MQ=255)                                                         ggggCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTg                                                                                                            >  1:152650/1‑98 (MQ=255)                                                         ggggCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTg                                                                                                            <  2:152650/98‑1 (MQ=255)                                                            tcATTACCCCTGAGGATGACTGGTTGCAGTCGGGTGGAAGGCAGGACTCGCCGGTGATGGAGTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCaaga                                                                    <  1:173210/134‑1 (MQ=255)                                                               ttACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCaagaag                                                                  >  2:37602/1‑134 (MQ=255)                                                                    ccTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGa                                                            <  4:308904/135‑1 (MQ=255)                                                                        aGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGTCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCa                                                        >  7:300498/1‑135 (MQ=255)                                                                        aGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGCCCAAGAAGCTAGGATTCa                                                        >  7:108231/1‑135 (MQ=255)                                                                        aGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGCAGCTAGGATTCa                                                        >  8:329436/1‑135 (MQ=255)                                                                        aGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCa                                                        >  7:209370/1‑135 (MQ=255)                                                                        aGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCa                                                        >  3:325689/1‑135 (MQ=255)                                                                        aGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCa                                                        >  5:313226/1‑135 (MQ=255)                                                                           aTGACTTGTTGCAGGCGGTTGGCAGTCCGGACTCGCCGGTGATTGACTTTCATTAACACTCGCGGACCAACGATCGCCTGCACTACCGCATCACCATGCCAGGTCGCCCGGAGAACAAGAAGCTAGGATTCAGcc                                                     <  7:310710/135‑1 (MQ=255)                                                                           aTGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGcc                                                     <  4:280105/135‑1 (MQ=255)                                                                              aCTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGCTTCAGCCGCa                                                  >  8:337423/1‑135 (MQ=255)                                                                                tGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAAt                                                >  5:30689/1‑135 (MQ=255)                                                                                   ttGCAGGCAGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATgg                                              >  4:15049/1‑134 (MQ=255)                                                                                    tGCCGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCt                                            <  5:126960/135‑1 (MQ=255)                                                                                        ggCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCA‑TACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTg                                       <  5:145712/135‑1 (MQ=255)                                                                                         gCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTg                                       <  1:37602/135‑1 (MQ=255)                                                                                             gTGGAAGGCAGGACTCGCCGGTGATGGACTTTCA‑TACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCtt                                  <  7:307799/135‑1 (MQ=255)                                                                                              tGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCtt                                  <  5:163402/135‑1 (MQ=255)                                                                                              tGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCtt                                  <  7:53619/135‑1 (MQ=255)                                                                                               ggAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCtt                                  >  1:281895/1‑134 (MQ=255)                                                                                               ggAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCtt                                  <  2:281895/134‑1 (MQ=255)                                                                                                 caGGCAGGACGCGCCGGGGATGGCCTTTCCTTACCCCTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTa                               <  2:180309/134‑1 (MQ=255)                                                                                                 aaGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTa                               >  6:320973/1‑135 (MQ=255)                                                                                                 aaGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTa                               >  1:20888/1‑135 (MQ=255)                                                                                                 aaGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTa                               <  6:30689/135‑1 (MQ=255)                                                                                                 aaGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTa                               <  3:164869/135‑1 (MQ=255)                                                                                                        gACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGAGCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTACTGGCAt                        <  5:305882/135‑1 (MQ=255)                                                                                                         aCTCGTCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTACTGGCAt                        <  8:300498/134‑1 (MQ=255)                                                                                                           tCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAANNAGNNAGGNTTCAGCCGCAATGGCTATCTGGGCTTCTACTGGCAt                        >  3:51248/1‑132 (MQ=255)                                                                                                              ccGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCCTCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTACTGGCATGCag                    <  3:164465/133‑1 (MQ=255)                                                                                                              ccGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTACTGGCATGCagag                  >  6:149644/1‑135 (MQ=255)                                                                                                                ggTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTACTGGCATGCAGAgg                 >  5:224816/1‑134 (MQ=255)                                                                                                                ggTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTACTGGCATGCAGAgg                 >  8:38648/1‑134 (MQ=255)                                                                                                                               ttACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATCGCTATCTGGGCTTCTACTGGCATGCAGAGGTTAGCGAATACTGGa  >  1:193981/1‑134 (MQ=255)                                                                                                                               |                                                                                                                                      CACCGTCGACCGGGACATCCCGCTACGCCTCAGCCGCAGTACAGACAAGTTCGGCTGGGGCATTACCCCTGAGGATGACTGGTTGCAGGCGGGTGGAAGGCAGGACTCGCCGGTGATGGACTTTCATTACCACTCGCGGACCAACGATCGCCTGCACTACCGCATCAGCATGCCAGGGCGCCCGGAGACCAAGAAGCTAGGATTCAGCCGCAATGGCTATCTGGGCTTCTACTGGCATGCAGAGGTTAGCGAATACTGGA  >  KT2440/5426032‑5426291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑