Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	12,483	G→A	9.4%	T618T (ACG→ACA)	CJ019_00019 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	12,483	0	G	A	9.4%	129.1 / 4.0	43	T618T (ACG→ACA)	CJ019_00019	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (18/20);  new base A (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GGTTTCGCCCCGTTGCGGGGCCATCCATTCCAAACGCGGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCATTTTGGAGAATCTTATGTCCCGTCTCG  >  KT2440/12353‑12615                                                                                                                                   |                                                                                                                                     ggTTTCGCCCCGTTGCGGGGCCATCCATTCCAAACGCGGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGttt                                                                                                                                   >  5:297067/1‑134 (MQ=255)        ccccGTTGCGGGGCCATCCATTCCAAACGCGGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCcac                                                                                                                           <  7:30208/135‑1 (MQ=255)          ccGTTGCGGGGCCATCCATTCCAAACGCGGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCacac                                                                                                                         >  3:238795/1‑135 (MQ=255)                cGGTGCCATCCATTCCAAACGCGGCTTGCCGGCAGTACGTTTGGGTAAGTAGCAAAAAGCTGATCGATTACGTCGCAAGTGAAGCCGCCCAGATCGTCGATCCTGAAGCCATCACGTTTTTATCCACACCGATc                                                                                                                    <  1:215347/134‑1 (MQ=255)                   ggCCATCCATTCCAAACGCTGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTc                                                                                                                <  6:117080/135‑1 (MQ=255)                     ccttccatTCCAAACGCGGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAg                                                                                                              >  7:48216/4‑135 (MQ=255)                           atTCCAAACGCGGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCg                                                                                                         >  4:246740/1‑134 (MQ=255)                           atTCCAAACGCGGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACATTTTTATCCACACCGATCGCTCAGCCTCg                                                                                                         >  3:86827/1‑134 (MQ=255)                           atTCCAAACGCGGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACATTTTTATCCACACCGATCGCTCAGCCTCg                                                                                                         >  3:91624/1‑134 (MQ=255)                                   cgcgGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCg                                                                                                <  4:298372/135‑1 (MQ=255)                                    gcgGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCCCGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGa                                                                                               >  5:138255/1‑135 (MQ=255)                                     cgGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGAt                                                                                              >  4:326499/1‑135 (MQ=255)                                        cTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCa                                                                                           <  6:332587/135‑1 (MQ=255)                                             cGGCAGTACGTTTTGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCa                                                                                       <  8:129145/134‑1 (MQ=255)                                             cGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCa                                                                                       <  3:310745/134‑1 (MQ=255)                                             cGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCa                                                                                       <  4:238795/134‑1 (MQ=255)                                             cGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACATTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCa                                                                                       <  4:91624/134‑1 (MQ=255)                                             cGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACATTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCa                                                                                       <  4:86827/134‑1 (MQ=255)                                                       tttGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCaa                                                                            <  8:266914/135‑1 (MQ=255)                                                        ttGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGAt                                                                               >  5:333162/1‑131 (MQ=255)                                                                   gCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCAc                                                                >  4:117960/1‑135 (MQ=255)                                                                            tGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGctttcttt                                                       <  3:14768/135‑1 (MQ=255)                                                                                 gATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGa                                                  >  1:157329/1‑135 (MQ=255)                                                                                 gATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGa                                                  >  7:92890/1‑135 (MQ=255)                                                                                      ccTCGCAAGTGAAGCCGACCAGATCGACGATCCTGAAGCCATCACTTTTTTATCCACACCGATCGCTCAGCCTCGTGATTTCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGc                                              >  6:102904/1‑134 (MQ=255)                                                                                       cTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTa                                            <  2:157329/135‑1 (MQ=255)                                                                                           cAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTaaaa                                         >  3:127063/1‑134 (MQ=255)                                                                                                          aGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCAtttt                         >  4:294964/1‑135 (MQ=255)                                                                                                          aGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCAtttt                         <  8:92890/135‑1 (MQ=255)                                                                                                          aGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCAtttt                         <  3:117960/135‑1 (MQ=255)                                                                                                          aGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCAtttt                         <  1:210829/135‑1 (MQ=255)                                                                                                            aTCGACGATCCTGAAGCCATCACGTTTTTCTCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCATTTTgg                       <  3:55018/135‑1 (MQ=255)                                                                                                            aTCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCATTTTgg                       <  2:282523/135‑1 (MQ=255)                                                                                                            aTCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCATTTTgg                       <  8:330649/135‑1 (MQ=255)                                                                                                                 cgaTCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCATTTTGGAGAAt                  <  7:203242/135‑1 (MQ=255)                                                                                                                         aaGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCAtttt                         <  5:28046/120‑1 (MQ=255)                                                                                                                         aaGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCAtttt                         >  6:28046/1‑120 (MQ=255)                                                                                                                                 aCGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAg                                                                   >  2:5298/1‑70 (MQ=255)                                                                                                                                 aCGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAg                                                                   <  1:5298/70‑1 (MQ=255)                                                                                                                                 aCGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCATTTTGGAGAATCTTATGTCCCGTCTCg  >  4:173989/1‑135 (MQ=255)                                                                                                                                 aCGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCATTTTGGAGAATCTTATGTCCCGTCTCg  >  1:31475/1‑135 (MQ=255)                                                                                                                                 aCGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCAGTTTGGAGAATCTTATGTCCCGTCTCg  >  8:250543/1‑135 (MQ=255)                                                                                                                                 aCGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATGGCTAAAATCATCGAACCCATTTTGGAGAATCTTATGTCCCGTCTCg  >  2:143231/1‑135 (MQ=255)                                                                                                                                   |                                                                                                                                     GGTTTCGCCCCGTTGCGGGGCCATCCATTCCAAACGCGGCTTGCCGGCAGTACGTTTGGGTAAGAAGCAAAAAGCTGATCGATTACCTCGCAAGTGAAGCCGCCCAGATCGACGATCCTGAAGCCATCACGTTTTTATCCACACCGATCGCTCAGCCTCGTGATTGCCGATTCAGGCATGTGGGATCAAGATAGTTAGCACGCTTTCTTTCTTGATTGCTAAAATCATCGAACCCATTTTGGAGAATCTTATGTCCCGTCTCG  >  KT2440/12353‑12615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑