| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,850,615 | T→G | 28.0% | V236G (GTG→GGG) | CJ019_05478 → | putative membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,850,615 | 0 | T | G | 28.0% | 64.0 / 4.3 | 61 | V236G (GTG→GGG) | CJ019_05478 | putative membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (24/18); new base G (3/16); total (27/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.65e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGG > KT2440/5850481‑5850747 | gcTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGt > 1:211540/1‑135 (MQ=255) ggCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTAGCCGCCGACCCGTTAATTCACCTGACCCCATCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCGTAGCCGTGccc > 8:135345/1‑135 (MQ=255) gCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCcg < 1:241776/135‑1 (MQ=255) gCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCcg > 8:109584/1‑135 (MQ=255) gCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCcg < 3:80867/135‑1 (MQ=255) tGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGCCCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCcgac > 8:260869/1‑134 (MQ=255) tACAGGGCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTGCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGGGCCCGACGAAg < 5:8906/135‑1 (MQ=255) aCTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGCCCCGTTCATTCCCCTGCCCCCAGCACTGGGCGCCCGGCCGTCGT‑TGGTGGGCTTCGCCGGGCCCGCCGACGc > 4:291599/1‑135 (MQ=255) tCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTGGGGTGGGCTTCGCCGGCCCCGCCGAGGCCagg > 7:323810/1‑133 (MQ=255) tCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCcg < 8:269909/123‑1 (MQ=255) tCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCcg > 7:269909/1‑123 (MQ=255) tCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGCCGAAGCCATg > 3:106414/1‑134 (MQ=255) tCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCGTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTAGGCGTGCCCGACGAAGCCATGa > 8:148632/1‑135 (MQ=255) tGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATcc < 8:176699/135‑1 (MQ=255) cTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGCCCCGTTCATTCACCTGACCCCAGCACTGGGCGCCCTCCCGTCGT‑TGGGGGGGGTGGCCGTGGCCGCCGGCGCCCTGATCcgc > 5:18031/1‑135 (MQ=255) cTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGCCCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCGCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGCCGAAGCCATGCTCcgc > 8:210344/1‑135 (MQ=255) ggCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGGTCATTCCCCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTGGCCGTGCCCGCCGCAGCCCTGATCCgcgc > 8:121627/1‑135 (MQ=255) cGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGCCCCGTTCATTCACCTGCCCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCGTCGCCGTGGCCGACGAAGCCAAGATCCGCGccc > 8:293962/1‑134 (MQ=255) cccGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCcgcg > 5:163091/1‑135 (MQ=255) ggCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAgcggcg < 2:211540/135‑1 (MQ=255) gcgcTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGCCCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCGCCCGTCGT‑GGCGGGGCTTCGCCGGGCCCGACGAAGCCATGATCCGCGCCAGCGGCGa < 7:4591/135‑1 (MQ=255) aTGGCTTTGCCTGGGAAACCACCTTGCGCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGGCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGcc < 6:18031/135‑1 (MQ=255) aTGGCTGTGTCTGGGAAACCACCTTGCGCGCCGCCGACCCGTACATTCCCCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTGCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGcc < 7:244069/135‑1 (MQ=255) ggCTTGGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCCCTGGGCGCCCTCCGGTGGT‑TGCGGGGCTGCGCCGTGCCCGACTAAGCCATGAGCCGCGCCAGCGGCGACAGcc < 6:30669/133‑1 (MQ=255) gggAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGGGCCCGGCGAAGCCAGGATACGCGCCAGCGGCGCCACCCAGCCACCCCtcg > 8:288595/1‑133 (MQ=255) gAAACCACCTTGCTCGCCGCCGACCCGTTCATTCCCCTGACCCAAGCACTGGGCGCCCGCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGAc < 3:22957/135‑1 (MQ=255) aaccccccTTGCGCGCCGCCGACCCGTTCTTACACCTGCCCCACGCAGGGGGCGCCCTCCCGTCGT‑TGCGGGGCTTCGCCGGGCCGGAGTAAGCCTGGATCCGCGCCCGCGGCGACAGCCTGCCAGCCCTGGACg < 6:48710/129‑1 (MQ=255) aaCCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGt > 8:58692/1‑135 (MQ=255) accaccTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGAACTGGGCACCCTCCCGTCGT‑TGCTTGGCTTCTCCGTGCCCGACCAAGCCATTATCAGCGCCAGCGGCGACAGCCATCAAGCCCTGTACGTg > 2:266422/1‑135 (MQ=255) accTTGCTCGCCGCCCACCCGTTCATACCCCTGACCCAAGCACGGGGCGCCCGCCCGGCGT‑TGCTGGGCTGCGCCGTCCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGcc < 3:291599/135‑1 (MQ=255) cTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGCCGCAGCCAAGATCCCCCCCAGCGGCGACACCCTGCCCCCCCGGGCCGTGGgccc > 6:36848/1‑134 (MQ=255) cgcgccgcCGACCCGTTCCTTCACCTGACCCACGCCCGGGGCGCCCGCCCGGCGG‑GGCGGGGCTGCGCCGGGCCCGACGAAGCCATGAGCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAg < 4:190644/133‑1 (MQ=255) cTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCa < 2:190740/134‑1 (MQ=255) ccgccgACCCGTCCATCCACCTGCCCCACGCCCTGGGCGCCCTCCCGTCGT‑TGCTGGGCGGCGCCGGGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGc < 3:327163/133‑1 (MQ=255) gccgACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCCGCCCGGGCCGTGGCCCGCCAGGCCggg > 7:141945/1‑135 (MQ=255) gTTCATCCCCCTGCCCCAAGCAGTGGGCGCCCTCCCGGCGT‑TGGTGGGCTGCGCGGGGCCCGACGAAGCCATGAGCCGCGCCCGCGGCGACAGCCGGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgctg < 4:290979/135‑1 (MQ=255) attcaCCTGACCCAAGCCCTGGGCGCCCGCCCGTCGT‑TGCTGGGCTTCGCCGGGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgctggct < 8:323810/134‑1 (MQ=255) attcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGGGCCCGACGAAGCCATGAGCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgctggct < 7:169604/134‑1 (MQ=255) aCCTCACCCAAGCACTGGGCGCCCGCCCGTCGT‑TGGTGGGCTTCGCCGGGCCCGACGAAGCCAGGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCg < 8:2120/135‑1 (MQ=255) ccTGACCCACGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGGGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCg < 3:283103/134‑1 (MQ=255) ccccAAGCACTGGGCGCCCTCCCGGGGT‑GGCGGGGCTGCGCCGGCCCCGACGACGCCATGAGCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtg < 7:314039/134‑1 (MQ=255) agaccggCACTGGCCGCCCTCCCGTCGT‑TGCGGGGCGGCGCCGGGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtg < 5:312172/129‑1 (MQ=255) aCCCATGCCCTGGGCGCCCGCCGGGCGT‑GGCGGGGCTGCGCGGGGCCCGACGATCCAAGGAGCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtg < 6:25984/135‑1 (MQ=255) aCCCACGCCCGGGCCGCCCTCCCGGCGT‑GGCTGGGCTTCGCCTTGCCCGACGAAGCCATGAGCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtg < 1:91365/135‑1 (MQ=255) aCCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtg < 7:109584/135‑1 (MQ=255) ccAACCACTGGGCGCCCTCCCGGCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCAGGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGCCGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtggt < 3:128904/135‑1 (MQ=255) cAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACCCCCTGCCCGCCCGGGCCGGTGCCCGCCAGGCCGGGGGCAGCTGGCGGCTCGGCgtgggg > 7:304163/1‑133 (MQ=255) ccTGGGCGCCCGCCCGTGGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGt < 8:134733/134‑1 (MQ=255) gcCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCCGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGc > 4:169726/1‑135 (MQ=255) cccGTCGT‑TGCTGGGCTTCGCCCTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCCGCCCTGGCCGTGGCCCGCCACGCCTGGGCCCGCGGGCTGCGCGGCGTGGTGCGGGGATACGGcggtcg > 6:312172/1‑130 (MQ=255) cccGTCGT‑GGCGGGGCTTCGCCGGGCCCGACGAAGCCAGGACCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCctgct < 7:210344/135‑1 (MQ=255) gtcgt‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGACCTGCTGcc > 7:82162/1‑135 (MQ=255) cgt‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGcccc < 7:260869/135‑1 (MQ=255) ggtgcgtGGGCTGCGCGGTGCACGTCGAAGCCTGGTTCCGCGCCAGCGGCGAGAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGcccc < 8:57284/129‑1 (MQ=255) gggCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCGGGCTGCTCGGCGTGGTGCTGGTATACCGCCTGCTGCCCCGCctgcg > 5:228209/1‑134 (MQ=255) ctgcGCCGGGCCCGACGACGCCAGGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctgct < 7:293962/132‑1 (MQ=255) cTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctgct < 6:163091/135‑1 (MQ=255) cTTCGCCGGGCCCGACGAAGCCATGATCCGCGCCCGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctgct < 5:56212/135‑1 (MQ=255) cGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCCCCCCGCCAGGGCCCGCAGGCTGCTAGGGGGGGTGGTGGGATAAGGCCTGCGTCCCCCCCGGCTGCTggg > 5:38576/1‑134 (MQ=255) cGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCCGGCCGGGGCCCGCTGGCTGCTCGGCGGGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCGGCTGGCCgg > 6:18693/1‑135 (MQ=255) cGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCgg > 8:202425/1‑135 (MQ=255) cGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGGGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCgg > 6:81868/1‑135 (MQ=255) | GCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGT‑TGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGG > KT2440/5850481‑5850747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |