Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,922,380 C→T 7.3% L168L (CTG→CTA)  CJ019_05543 ← putative binding protein component of ABC iron transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,922,3800CT 7.3% 176.3 / 2.2 54L168L (CTG→CTA) CJ019_05543putative binding protein component of ABC iron transporter
Reads supporting (aligned to +/- strand):  ref base C (27/23);  new base T (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTGCCCGGCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCGGGCG  >  KT2440/5922249‑5922512
                                                                                                                                   |                                                                                                                                    
catTGCCCGGCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGc                                                                                                                                   <  1:89511/135‑1 (MQ=255)
catTGCCCGGCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGc                                                                                                                                   <  2:94704/135‑1 (MQ=255)
    gCCCGGCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTTAGCGACt                                                                                                                               >  5:337905/1‑135 (MQ=255)
    gCCCGGCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTTAGCGACt                                                                                                                               >  5:337909/1‑135 (MQ=255)
         gCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTg                                                                                                                          <  4:178313/135‑1 (MQ=255)
                               cgTTGTCGTCGGAGACCACCTCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCa                                                                                                    >  4:331474/1‑135 (MQ=255)
                               cgTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCa                                                                                                    >  8:162798/1‑135 (MQ=255)
                                       tcgGAGACCACAGCGGGGGACAGGTGGTTGACCCACCCTGTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGGTTCGAGCAGGGGGGCGGTCAGCGACTGGGTGTACACCTTCTTGGCCGTGCGCAGGCACAGa                                                                                            <  7:7400/135‑1 (MQ=255)
                                                 cctCGGTGGACAGGTTGTTGACCCACCCTTTGAAGATTTGCTAGGTCTTGGCCTCACCGTGGGTTTCGAGCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTccc                                                                                  <  7:138490/133‑1 (MQ=255)
                                                 caTCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGACTCACAGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGCCGGCCTTccc                                                                                  >  4:308464/1‑135 (MQ=255)
                                                     ggTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGCCGGCCTTCCCACTg                                                                              >  6:236990/1‑135 (MQ=255)
                                                      gTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGt                                                                             <  6:22204/135‑1 (MQ=255)
                                                              gttgttGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGTTCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGc                                                                     <  3:331474/135‑1 (MQ=255)
                                                                 gttgACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAg                                                                  >  7:78783/1‑135 (MQ=255)
                                                                      cccacccTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTTAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCtt                                                             <  6:337909/135‑1 (MQ=255)
                                                                      cccacccTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCtt                                                             <  3:24756/135‑1 (MQ=255)
                                                                      cccacccTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCtt                                                             <  1:82601/135‑1 (MQ=255)
                                                                      cccacccTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCtt                                                             <  8:247491/135‑1 (MQ=255)
                                                                      cccacccTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGGGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCtt                                                             <  8:131173/135‑1 (MQ=255)
                                                                      cccaccaTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTTAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCGTCCCACTGTTTGTCAGCCAGGGCtt                                                             <  6:337905/135‑1 (MQ=255)
                                                                         acccTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCg                                                                                                                                  >  4:13942/1‑63 (MQ=255)
                                                                         acccTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCg                                                                                                                                  <  3:13942/63‑1 (MQ=255)
                                                                           ccTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTg                                                                                                                          >  8:33615/1‑69 (MQ=255)
                                                                           ccTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTg                                                                                                                          <  7:33615/69‑1 (MQ=255)
                                                                           ccTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAg                                                        >  4:142386/1‑135 (MQ=255)
                                                                                     tttGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAAct                                              <  8:113795/135‑1 (MQ=255)
                                                                                        gCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAActcct                                           >  5:157447/1‑135 (MQ=255)
                                                                                          tCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCg                                         >  3:328108/1‑135 (MQ=255)
                                                                                          tCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCg                                         >  6:24521/1‑135 (MQ=255)
                                                                                                    ccTCACGGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGAccc                               <  5:24521/135‑1 (MQ=255)
                                                                                                        cccGTGGGTTTCGATCAGGGGGGCGGGCAGCGACTGGATGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGGCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTc                           <  6:202895/134‑1 (MQ=255)
                                                                                                         ccGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCg                          <  7:162798/135‑1 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTc                                                                        <  2:330933/87‑1 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTc                                                                        >  1:330933/1‑87 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                        >  8:110737/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                        >  1:165122/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                        >  8:306608/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                        >  1:169087/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                        >  8:233528/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                        >  5:339385/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                        >  6:239830/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                        >  7:136016/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                        >  3:222356/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTAGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                        >  3:239420/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGAGGTCAGCGACTGGTTGTACACCTTCTTGGACGTGCGCAGGAACAGAAGGCCTTCAAACTGTTTGTCAGACAGGTCTTCGTAGGTGCTCAACTACTCGGGTTTGACAAGCTCGGt                        >  8:225564/1‑135 (MQ=255)
                                                                                                                 ttCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGa                     >  1:208581/1‑132 (MQ=255)
                                                                                                                    gATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAga                <  4:328108/134‑1 (MQ=255)
                                                                                                                     aTCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAgatg              >  2:162876/1‑135 (MQ=255)
                                                                                                                     aTCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAgatg              >  5:237923/1‑135 (MQ=255)
                                                                                                                             ggCGGTCAGCGACTGGTTGTACACCATCTAGGCCGAGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGCTCGAGTAGATGATGGTGCg      >  6:90448/1‑135 (MQ=255)
                                                                                                                                ggTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCGGgc   <  2:169087/135‑1 (MQ=255)
                                                                                                                                ggTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCGGgc   <  2:165122/135‑1 (MQ=255)
                                                                                                                                ggTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCGGgc   <  8:136016/135‑1 (MQ=255)
                                                                                                                                 gTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCGGgcg  <  5:43154/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                    
CATTGCCCGGCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCGGGCG  >  KT2440/5922249‑5922512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑