| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 6,133,865 | C→G | 10.5% | P412R (CCT→CGT) | cusB → | Cation efflux system protein CusB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 6,133,865 | 0 | C | G | 10.5% | 123.6 / 6.0 | 38 | P412R (CCT→CGT) | cusB | Cation efflux system protein CusB |
| Reads supporting (aligned to +/- strand): ref base C (13/21); new base G (2/2); total (15/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTGGGATGACCATGACCTTTCCCGTCGCTGATCCC > KT2440/6133733‑6133995 | tCGCAATCTTGAACGGCCGGCCGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTg < 8:103742/135‑1 (MQ=255) aGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGc < 6:305328/134‑1 (MQ=255) gCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGc > 4:271980/1‑135 (MQ=255) aGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCgg > 6:85118/1‑135 (MQ=255) aGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCgg > 5:327450/1‑135 (MQ=255) ggCGGGGCAGAATGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGa < 1:285941/135‑1 (MQ=255) gCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACGTGAACCCGAACTGCACCAAGCGGATGGcca > 4:65929/1‑134 (MQ=255) gCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACGTGAACCCGAACTGCACCAAGCGGATGGcca > 4:61267/1‑134 (MQ=255) aaaGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTc < 3:271980/135‑1 (MQ=255) cGTGGCTTCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATGGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACa < 4:112455/135‑1 (MQ=255) ggCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAAt < 3:215512/135‑1 (MQ=255) cATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCACGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCg < 4:124327/135‑1 (MQ=255) cATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCg < 2:46323/135‑1 (MQ=255) tCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGc < 3:181352/135‑1 (MQ=255) cTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTg > 3:235808/1‑135 (MQ=255) tGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGa < 1:127726/135‑1 (MQ=255) gACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATc > 2:125634/1‑135 (MQ=255) aagcaagcCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACg < 3:124365/135‑1 (MQ=255) ccTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCAtt < 8:281923/135‑1 (MQ=255) cTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTc > 1:299838/1‑135 (MQ=255) cTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTc > 8:312005/1‑135 (MQ=255) aGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGaa > 5:338561/1‑53 (MQ=255) aGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGaa < 6:338561/53‑1 (MQ=255) ggCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAAt < 6:327450/135‑1 (MQ=255) ccacAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTg < 7:312005/135‑1 (MQ=255) ccacAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTg > 2:235637/1‑135 (MQ=255) cacaGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCaa < 7:84137/55‑1 (MQ=255) cacaGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCaa > 8:84137/1‑55 (MQ=255) acaGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTggg > 4:295294/1‑135 (MQ=255) acaGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGCCACCCAGCTGACCATCGACCACGGGCCATTAGTCACCTTGGGAATGCCTggg > 3:16807/1‑135 (MQ=255) gCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTGGGatg < 7:7558/135‑1 (MQ=255) aTCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTGGGatgac < 3:295294/135‑1 (MQ=255) gATTCACCGCCTGCCTCGCAAGCACGTGAACCCGAACTGCACCAAGCGGATGGCCATGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTGGGatgaccatg < 3:65929/135‑1 (MQ=255) gATTCACCGCCTGCCTCGCAAGCACGTGAACCCGAACTGCACCAAGCGGATGGCCATGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTGGGatgaccatg < 3:61267/135‑1 (MQ=255) ccGCCTGGCTCGCAAGGACCTGAACCCGAACTGCACCAAGCGGATGGGCGTGTCGTACAAATCGCAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTGGGATGAGCATGACCttt < 4:235808/135‑1 (MQ=255) ctCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGCCCACGGGCCATTCGTCACCTTGGGAATGCCAGGGATGACCATCACCTTTCCCGTc > 4:230503/1‑133 (MQ=255) cAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTGGGATGACCATGACCTTTCCCGTCGCTg < 5:85118/133‑1 (MQ=255) gCACCTGAACCCGAACTGCACCAAGCGGATGGGCGTGTCGTACATATCGAAGGCAGCCAGCTGACCTTCGACCACGGGCCATTGGTCAGCTTGGGAATGCCTGGGATGACCATGACCTTTCCCGTCGCTGATccc < 6:22916/135‑1 (MQ=255) | TCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTGGGATGACCATGACCTTTCCCGTCGCTGATCCC > KT2440/6133733‑6133995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |