Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 383,395 G→A 8.5% G207S (GGC→AGC)  soxA_1 → Sarcosine oxidase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440383,3950GA 8.5% 146.0 / 4.0 49G207S (GGC→AGC) soxA_1Sarcosine oxidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (23/21);  new base A (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

AGCTACGACTACATGAACCGGCACTGCGACGTGCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCCTGCTGCCACGTGCCACGGTCAACGGCTACCACGAC  >  KT2440/383263‑383529
                                                                                                                                    |                                                                                                                                      
aGCTACGACTACATGAACCGGCACTGCGACGTGCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCggcggc                                                                                                                                      <  5:238397/135‑1 (MQ=255)
     cGACTACATGAACCGGCACTGCGACGTGCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCt                                                                                                                                 >  2:234117/1‑135 (MQ=255)
     cGACTACATGAACCGGCACTGCGACGTGCTGATCGTCGGCGCCGGACCTGCTGGCCTGTCTTCCGCACTGGCCGCTGCGCGCAGTTGTGCCCGCCTGATACTGGCTGACGAGCAGGAAGAGTTCGGCCGCAGccg                                                                                                                                 >  1:166119/1‑134 (MQ=255)
      gACTACATGAACCGGCACTGCGACGTGCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGCGCAGGAAGAGTTCGGCAGCAGCCTg                                                                                                                                >  2:19931/1‑135 (MQ=255)
      gACTACATGAACCGGCACTGCGACGTGCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCAGCAGCCTg                                                                                                                                >  2:13888/1‑135 (MQ=255)
      gACTACATGAACCGGCACTGCGACGTGCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCAGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGGAGGAAGAGTTCGGCGgccgccag                                                                                                                                >  6:38023/1‑133 (MQ=255)
             tGAACCGGCACTGCGACGTGCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGaca                                                                                                                         >  4:105268/1‑135 (MQ=255)
                        tGCGACGTGCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCGGGCTGCTCGCGCCCCGCGAAAc                                                                                                               >  1:283416/1‑134 (MQ=255)
                        tGCGACGTGCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACCCCCGCGAAAcc                                                                                                              >  1:224021/1‑135 (MQ=255)
                             cGTGCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGa                                                                                                         >  7:160065/1‑135 (MQ=255)
                             cGTGCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGa                                                                                                         >  1:22075/1‑135 (MQ=255)
                              ggggtGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGAc                                                                                                        <  7:146135/131‑1 (MQ=255)
                                gCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACgg                                                                                                      <  6:335864/135‑1 (MQ=255)
                                  tGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCa                                                                                                    <  5:120560/135‑1 (MQ=255)
                                  tGATCGGCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGGGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCa                                                                                                    <  8:133641/135‑1 (MQ=255)
                                       gtcgGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGCAACCCTCGACGGCAAGCct                                                                                               >  3:280082/1‑135 (MQ=255)
                                        tcgGCGCCGGACCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGGGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGGTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCctg                                                                                              <  6:160464/135‑1 (MQ=255)
                                             gcCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGcc                                                                                         <  4:150949/135‑1 (MQ=255)
                                             gcCGGCCCTGCTGGCCGGGCTGCCGCACTGGCCGCTGCGCGCAGGGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGcc                                                                                         <  5:270265/135‑1 (MQ=255)
                                             gcCGGCCCTGCGGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGGGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGcc                                                                                         <  3:259116/135‑1 (MQ=255)
                                                         ggcctggcTGCCGCACTGGCCGCTGCGCGCAGTGGTGCGCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAAc                                                                             <  2:22075/135‑1 (MQ=255)
                                                          gcctggcTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACg                                                                            <  3:252493/135‑1 (MQ=255)
                                                          gcctggcGGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACg                                                                            <  6:311824/135‑1 (MQ=255)
                                                                 tGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGt                                                                     <  8:227081/135‑1 (MQ=255)
                                                                  gCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGt                                                                     >  2:178408/1‑134 (MQ=255)
                                                                  gCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGt                                                                     >  1:107229/1‑134 (MQ=255)
                                                                  gCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCAGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTc                                                                    <  1:19931/135‑1 (MQ=255)
                                                                    cGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCAGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTc                                                                    <  1:13888/133‑1 (MQ=255)
                                                                    cGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTGCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTc                                                                    <  8:259615/133‑1 (MQ=255)
                                                                         tGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGc                                                             <  8:160065/135‑1 (MQ=255)
                                                                              gCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAACCCCTCGACGGCAAGCCTGCTGCCGACTGGGTCCACGCCGTGGTCAAAGAGCTGGaa                                                        >  1:173772/1‑135 (MQ=255)
                                                                              gCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGaa                                                        >  4:288559/1‑135 (MQ=255)
                                                                              gCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGGTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGaa                                                        <  3:161493/135‑1 (MQ=255)
                                                                                   gcgcAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCACGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCt                                                   >  2:286990/1‑135 (MQ=255)
                                                                                   gcgcAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCt                                                   >  8:16755/1‑135 (MQ=255)
                                                                                   gcgcAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCt                                                   >  1:186778/1‑135 (MQ=255)
                                                                                   gcgcAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCt                                                   >  4:61165/1‑135 (MQ=255)
                                                                                         tggtgCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGGTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGa                                             <  7:64485/135‑1 (MQ=255)
                                                                                           gtgCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAg                                           <  4:231076/135‑1 (MQ=255)
                                                                                            tgCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAg                                           >  7:214368/1‑134 (MQ=255)
                                                                                                 gcgTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGAccc                                     >  7:5353/1‑135 (MQ=255)
                                                                                                 gcgTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGAccc                                     >  6:302171/1‑135 (MQ=255)
                                                                                                 gcgTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGAccc                                     >  3:164826/1‑135 (MQ=255)
                                                                                                 gcgTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGAccc                                     >  1:319122/1‑135 (MQ=255)
                                                                                                 gcgTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGAccc                                     <  1:234117/135‑1 (MQ=255)
                                                                                                      aTCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCctgctg                                >  2:24753/1‑135 (MQ=255)
                                                                                                      aTCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCctgctg                                >  6:258791/1‑135 (MQ=255)
                                                                                                             cTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCCTGCtgccacgtg                         <  2:107229/135‑1 (MQ=255)
                                                                                                             cTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCCTGCtgccacgtg                         <  1:295056/135‑1 (MQ=255)
                                                                                                             cTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCCTGCtgccacgt                          <  3:288559/134‑1 (MQ=255)
                                                                                                                                 gggggcAGCCTGCCCGACACCAGCTAAAACCTCGACGGCACGCCTGCTGCCGACTGTGTCAACGCCGTGGTCATAGAGCGGGAAGGCCTGCGGGAAGTGACCCTGCTGCCACGTGCCACGGTCAACGGCTACCa      <  8:157265/131‑1 (MQ=255)
                                                                                                                                 ggcggcAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCCTGCTGCCACGTGCCACGGTCAACGGCTACCAc     >  1:321237/1‑135 (MQ=255)
                                                                                                                                 ggcggcAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCCTGCTGCCACGTGCCACGGTCAACGGCTACCAc     >  1:209171/1‑135 (MQ=255)
                                                                                                                                   cggcAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCATCGCCGTGGTCACAGAGCTGGAAGGCATGCCGGAAGTGACGCTGCTGCCTCGTGCCACGGTCACCGGCTACcgcgc   >  5:258084/1‑134 (MQ=255)
                                                                                                                                   cggcAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCCTGCTGCCACGTGCCACGGTCAACGGCt          <  3:88636/128‑1 (MQ=255)
                                                                                                                                   cggcAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCCTGCTGCCACGTGCCACGGTCAACGGCt          >  4:88636/1‑128 (MQ=255)
                                                                                                                                   cggcAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCATGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCCTGCTGCCACGTGCCACGGTCAACGGCTACCACGa   >  7:203257/1‑135 (MQ=255)
                                                                                                                                    ggcAGCCGGCTCGACACCCGCGAACCCCTCGACGGCACGCCTGCTGCCGACTGGGTCAACGCCGGGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCCTGCTGCCACGTGCCACGGTCAACGGCTACCACGAc  <  1:139574/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
AGCTACGACTACATGAACCGGCACTGCGACGTGCTGATCGTCGGCGCCGGCCCTGCTGGCCTGGCTGCCGCACTGGCCGCTGCGCGCAGTGGTGCCCGCGTGATCCTGGCTGACGAGCAGGAAGAGTTCGGCGGCAGCCTGCTCGACACCCGCGAAACCCTCGACGGCAAGCCTGCTGCCGACTGGGTCAACGCCGTGGTCAAAGAGCTGGAAGGCCTGCCGGAAGTGACCCTGCTGCCACGTGCCACGGTCAACGGCTACCACGAC  >  KT2440/383263‑383529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑