Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	573,463	C→T	7.6%	C258C (TGC→TGT)	fdhE →	Protein FdhE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	573,463	0	C	T	7.6%	166.8 / 2.3	52	C258C (TGC→TGT)	fdhE	Protein FdhE
Reads supporting (aligned to +/- strand):  ref base C (31/17);  new base T (2/2);  total (33/19)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

GTGGCATGTGGTGCGGGTCAAGTGTGTGTACTGCGAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTGGATATACGTCTGGACGGGGAGGGCTTCCATCGCCTGGCGCCCAACCTGAT  >  KT2440/573337‑573594                                                                                                                               |                                                                                                                                    gTGGCATGTGGTGCGGGTCAAGTGTGTGTAGTGCGAGTCGTGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGTCAGCt                                                                                                                              <  8:295189/134‑1 (MQ=255) gTGGCATGTGGTGCGGGTCAAGTGTGTGTACTGCGAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTa                                                                                                                             >  5:181232/1‑135 (MQ=255)     cATGTGGTGCGGGTCAAGTGTGTGTACTGCGAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTg                                                                                                                         <  2:105253/135‑1 (MQ=255)            tgCGGGTCAAGTGTGTGTACTGCGAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGa                                                                                                                  <  1:245568/135‑1 (MQ=255)             gCGGGTCAAGTGTGTGTACTGCGAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGaa                                                                                                                 >  1:77736/1‑135 (MQ=255)               gggTCAAGTGTGTGTACTGCGAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACt                                                                                                               >  1:52443/1‑135 (MQ=255)                              aCTGCGAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATg                                                                                                >  3:290119/1‑135 (MQ=255)                              aCTGCGAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATg                                                                                                >  3:139118/1‑135 (MQ=255)                              aCTGCGAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATg                                                                                                >  7:173191/1‑135 (MQ=255)                                   gAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGTGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATgcggcg                                                                                           >  1:301184/1‑135 (MQ=255)                                   gAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGTGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATgcggcg                                                                                           >  1:305379/1‑135 (MQ=255)                                   gAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGTGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATgcggcg                                                                                           <  2:301184/135‑1 (MQ=255)                                   gAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGTGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATgcggcg                                                                                           <  2:305379/135‑1 (MQ=255)                                   gAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTAcc                                                                                                                           >  1:335057/1‑103 (MQ=255)                                   gAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTAcc                                                                                                                           <  2:335057/103‑1 (MQ=255)                                    aGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTAcc                                                                                                                           <  2:216689/102‑1 (MQ=255)                                    aGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTAcc                                                                                                                           >  1:216689/1‑102 (MQ=255)                                    aGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGcggcgg                                                                                          >  7:56380/1‑135 (MQ=255)                                    aGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGcggcgg                                                                                          >  3:74817/1‑135 (MQ=255)                                    aGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGcggcgg                                                                                          >  1:4706/1‑135 (MQ=255)                                    aGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGAAGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGcggcgg                                                                                          >  7:289950/1‑135 (MQ=255)                                        gAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGCACGATGCGGCGGCCGa                                                                                      >  4:113443/1‑135 (MQ=255)                                            aaGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCg                                                                                  >  8:157161/1‑135 (MQ=255)                                            aaGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCg                                                                                  >  6:55982/1‑135 (MQ=255)                                                aTTTGCGTTACACCCGCCTTGAGGATTATCGCCATGCGCCAGGCAACGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTgg                                                                              <  5:294500/135‑1 (MQ=255)                                                             cAGCCTTGAGTATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGa                                                                                                  >  3:201562/1‑102 (MQ=255)                                                             cAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGa                                                                                                  <  4:201562/102‑1 (MQ=255)                                                               gCCTTGAGGATGATCGCCATGCGCCAGGCAACGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCa                                                               <  4:74817/135‑1 (MQ=255)                                                                  ttGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTg                                                                                                                         <  8:228909/74‑1 (MQ=255)                                                                  ttGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTg                                                                                                                         >  7:228909/1‑74 (MQ=255)                                                                  ttGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCCGcc                                                            >  5:4033/1‑135 (MQ=255)                                                                  ttGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAccc                                                            >  1:275834/1‑135 (MQ=255)                                                                  ttGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGcc                                                            >  3:251808/1‑135 (MQ=255)                                                                  ttGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGcc                                                            >  8:193204/1‑135 (MQ=255)                                                                  ttGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGcc                                                            >  2:294964/1‑135 (MQ=255)                                                                     aGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTg                                                          <  5:159350/134‑1 (MQ=255)                                                                     aGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTg                                                          <  7:29316/134‑1 (MQ=255)                                                                     aGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGAGGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTg                                                          <  3:239736/134‑1 (MQ=255)                                                                     aGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGGGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTg                                                          <  2:5783/134‑1 (MQ=255)                                                                            tCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTgg                                                   <  2:77736/134‑1 (MQ=255)                                                                             cGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGTCGCTGGCCGAGGCCCTGGCCAGCCTGGcccg                                                     >  6:21712/1‑130 (MQ=255)                                                                             cGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTGGat                                                 >  1:293244/1‑135 (MQ=255)                                                                              gCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTGGat                                                 >  8:219916/1‑134 (MQ=255)                                                                              gCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTGGat                                                 >  1:228556/1‑134 (MQ=255)                                                                                     gcCAGGCAAAGCGCCGCTGCGCGCGGAATGCTTCCCCGGGTGCGACTGCTACCTTAAGCAGAACTACCTGGAGAAGGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTGGATATACGTCt                                         <  6:73092/135‑1 (MQ=255)                                                                                     gcCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTGGATATACGTCt                                         <  7:193204/135‑1 (MQ=255)                                                                                          gaaaaGCGCCGCGGCGCGCGGATTGCGGCCCGGGGGGCGACAGCTACCTTAAGCAGACCTACCTGGAGAACGAGGGGGGGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTGGATATACGTCTGGACg                                    <  7:134800/133‑1 (MQ=255)                                                                                             aaGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTGGATATACGTCTGGACgg                                   >  2:297549/1‑133 (MQ=255)                                                                                             aaGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTGGATATACGTCTGGACgg                                   >  8:268376/1‑133 (MQ=255)                                                                                                                     gcCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGCTGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTGGATATACGTCTGGACGGGGAGGGCTTCCATCGCCTGGCGCCCa         >  4:150769/1‑135 (MQ=255)                                                                                                                            gTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGCCCTGGCCAGCCTGGCCCTGGATATACGTCTGGACGGGGAGGGCTTCCATCGCCTGGCGCCCAACCTGAt  >  4:157525/1‑135 (MQ=255)                                                                                                                            gTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTGGATATACGTCTGGACGGGGAGGGCTTCCATCGCCTGGCGCCCAACCTGAt  >  4:329869/1‑135 (MQ=255)                                                                                                                               |                                                                                                                                    GTGGCATGTGGTGCGGGTCAAGTGTGTGTACTGCGAGTCGAGCAAGGATTTGCGTTACACCAGCCTTGAGGATGATCGCCATGCGCCAGGCAAAGCGCCGCTGCGCGCGGAATGCTGCCCCGGGTGCGACAGCTACCTGAAGCAGAACTACCTGGAGAACGATGCGGCGGCCGAGCCGCTGGCCGATGACCTGGCCAGCCTGGCCCTGGATATACGTCTGGACGGGGAGGGCTTCCATCGCCTGGCGCCCAACCTGAT  >  KT2440/573337‑573594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑