Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 635,862 G→A 8.3% R59C (CGC→TGC)  acoC ← Dihydrolipoyllysine‑residue acetyltransferasecomponent of acetoin cleaving system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440635,8620GA 8.3% 143.4 / 4.1 48R59C (CGC→TGC) acoCDihydrolipoyllysine‑residue acetyltransferasecomponent of acetoin cleaving system
Reads supporting (aligned to +/- strand):  ref base G (26/18);  new base A (2/2);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

GGCGACGAACTCGGCCTGGAAGCGCTGTACCACGGCATCGATCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTCCTGCTTGAGCCAGGTGTCCACCCGGCCCTCGGTCATCGACA  >  KT2440/635734‑635996
                                                                                                                                |                                                                                                                                      
ggCGACGAACTCGGCCTGGAAGCGCTGTACCACGGCATCGATCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCgcagc                                                                                                                                    <  4:59018/133‑1 (MQ=255)
      gaACTCGGCCTGGAAGCGCTGTACCACGGCATCGATCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCt                                                                                                                            <  8:94982/135‑1 (MQ=255)
         cTCGGCCTGGAAGCGCTGTACCACGGCATCGATCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGaa                                                                                                                         <  2:235702/135‑1 (MQ=255)
                    aGCGCTGTACCACGGAAACGATCTCGCCCTCCTCAGATACGACATCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGCGTATCATCCCCCTTGGCTAACTGGCGGCGAAGCACGCCGCTCAAAGGCGATTCGa                                                                                                              >  2:102718/1‑135 (MQ=255)
                          gTACCCCGGCATCGATCGCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACgctgc                                                                                                        <  5:225538/135‑1 (MQ=255)
                           tACCACGGCATCGATCTCGGCCTCCACTGAATCGCCTTCGACTACCCCCGCCAGCAGTGCGCCGACGGGCAGGGATTCATCCGGCATGGCTACCTGGCGGCGCAGCACGCCGATGAAAGGCGCATCGACgctgct                                                                                                       >  3:233829/1‑135 (MQ=255)
                                cGGCATCGATCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGa                                                                                                  >  1:273488/1‑135 (MQ=255)
                                   cATCGATCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCt                                                                                               <  4:96285/135‑1 (MQ=255)
                                   cATCGAGCTCGGCCTCCTCTGCATCGCGTGCGACTACCACCGCCATCAGTGCGCCGAGGGTAAGGGTTTCATCGGGCTTGGATACCGGGCGGCGAAGCACGACGCTGAAAGGCGCTTAGACGCTGCTGCTGATCt                                                                                               <  1:245445/135‑1 (MQ=255)
                                     tCGATCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTg                                                                                             >  3:253092/1‑135 (MQ=255)
                                      cGATCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGt                                                                                            >  6:245188/1‑135 (MQ=255)
                                      cGATCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCGGAAAGGCGCTTCGACGCTTCTGCTGATCtt                                                                                              >  5:15600/1‑133 (MQ=255)
                                        aTCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCCGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCg                                                                                          >  3:219896/1‑135 (MQ=255)
                                        aTCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAACGGCGCTTCGACGCTGCTGCTGATCTTGTCg                                                                                          >  7:69379/1‑135 (MQ=255)
                                        aTCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCg                                                                                          >  8:156965/1‑135 (MQ=255)
                                        aTCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCg                                                                                          >  8:169474/1‑135 (MQ=255)
                                        aTCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCg                                                                                          >  3:111488/1‑135 (MQ=255)
                                        aTCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCAGGCTTGGCTACCTGGCGGCGCAGCACGCCCCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGtag                                                                                          >  5:194345/1‑133 (MQ=255)
                                           tcGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGtc                                                                                       <  8:313292/135‑1 (MQ=255)
                                              gCCTCCTCTGCTTCGCCTTCGACTACCACCGCCCGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGtc                                                                                       <  4:219896/132‑1 (MQ=255)
                                                ctcctcTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGAc                                                                                  <  8:331530/135‑1 (MQ=255)
                                                 tcctcTGCTTCGCCTTCGACTACAACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCAGAAAGGCGCTTAGACGCTGCTGCTGATCTTGTCGGTCTCGACg                                                                                 >  2:271890/1‑135 (MQ=255)
                                                    tctGCTGCGCCTTCGACTCCCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTcc                                                                              <  1:64676/135‑1 (MQ=255)
                                                              cTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCACCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGCCGTCCAGCACCgcgt                                                                    >  5:239926/1‑135 (MQ=255)
                                                                tCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGCTCTTGTCGGTCTCGACGTCCAGCACCtcgtcg                                                                  >  3:88364/1‑135 (MQ=255)
                                                                tCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGGAACacgtcg                                                                  >  1:316917/1‑135 (MQ=255)
                                                                tCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCCCCtcgtcg                                                                  >  3:14759/1‑135 (MQ=255)
                                                                   aCTACCACCGCCAGCAGTGCGCCGACGGGCAGGGGTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCCCCTCGTCGccc                                                               >  7:245108/1‑135 (MQ=255)
                                                                     tACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCtt                                                             <  8:97851/135‑1 (MQ=255)
                                                                     tACCACCGCCAGCAGTGCACCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCtt                                                             >  4:90078/1‑135 (MQ=255)
                                                                          ccGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGa                                                        <  4:302720/135‑1 (MQ=255)
                                                                           cGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGAt                                                       >  4:298661/1‑135 (MQ=255)
                                                                           cGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCACAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGAt                                                       >  3:275423/1‑135 (MQ=255)
                                                                           cGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCACAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGAt                                                       >  3:280086/1‑135 (MQ=255)
                                                                                  agTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCa                                                <  4:111488/135‑1 (MQ=255)
                                                                                       gcCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGCTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTc                                           >  4:218829/1‑135 (MQ=255)
                                                                                         cGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTCCt                                         <  7:169474/135‑1 (MQ=255)
                                                                                         cGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTCCt                                         <  8:266140/135‑1 (MQ=255)
                                                                                              ggCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCACAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTCCTGCTTg                                    <  4:275423/135‑1 (MQ=255)
                                                                                              ggCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCACAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTCCTGCTTg                                    <  4:280086/135‑1 (MQ=255)
                                                                                               gCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTCCTGCTTGa                                   <  8:136431/135‑1 (MQ=255)
                                                                                               gCAGGGTGTCTTCCGGATTGTCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCAACTCGTCGGCCTTGCTGATCTCGTGACATTCCTGCttca                                   <  1:102718/135‑3 (MQ=255)
                                                                                                cAGGGGTTCCTCCGGCTTGGCTAGCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCTGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTCCTGCTTGAg                                  <  5:123040/135‑1 (MQ=255)
                                                                                                 aGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTCCTGCTTGAg                                  >  3:123733/1‑134 (MQ=255)
                                                                                                           ccGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGa                                                                                                              <  4:206493/48‑1 (MQ=255)
                                                                                                           ccGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGa                                                                                                              >  3:206493/1‑48 (MQ=255)
                                                                                                                  ggCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCCCCTCGTCGCCCGTGCTGATTTCGTCCCCTTCCTGCTTGAGCCAGGGGTCCACCCCGcc                >  2:319721/1‑135 (MQ=255)
                                                                                                                  ggCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTCCTGCTTGAGCCAGGTGTCCACCCGGcc                >  5:306391/1‑135 (MQ=255)
                                                                                                                      aCCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTCCTGCTTGAGCCAGGTGTCCACCCGGCCCTCg            >  5:229221/1‑135 (MQ=255)
                                                                                                                            cggcgCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTCCTGCTTGAGCCAGGTGTCCACCCGGCCCTCGGTCAt       <  4:233829/134‑1 (MQ=255)
                                                                                                                                gcagcaCGCCGATGAAAGGCGCTTCGCCGCTGCTGCTGATCTTGTCGGTCTCGCCGTCCAGCACCTCGTCGCCCTTGCTGAGTTCGTCCCCGTCATGCTGGAGCCAGGTGTCCACCCGGCCCGCGGTCCTCGACa  >  4:204264/1‑135 (MQ=255)
                                                                                                                                |                                                                                                                                      
GGCGACGAACTCGGCCTGGAAGCGCTGTACCACGGCATCGATCTCGGCCTCCTCTGCTTCGCCTTCGACTACCACCGCCAGCAGTGCGCCGACGGGCAGGGTTTCATCCGGCTTGGCTACCTGGCGGCGCAGCACGCCGCTGAAAGGCGCTTCGACGCTGCTGCTGATCTTGTCGGTCTCGACGTCCAGCACCTCGTCGCCCTTGCTGATTTCGTCACCTTCCTGCTTGAGCCAGGTGTCCACCCGGCCCTCGGTCATCGACA  >  KT2440/635734‑635996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑