Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,118,296 T→G 10.7% T435P (ACC→CCC)  tdcG_2 ← L‑serine dehydratase TdcG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,118,2960TG10.7% 149.6 / 3.4 74T435P (ACC→CCC) tdcG_2L‑serine dehydratase TdcG
Reads supporting (aligned to +/- strand):  ref base T (18/47);  new base G (7/2);  total (25/49)
Fisher's exact test for biased strand distribution p-value = 5.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

AGTGGGGTCTTGAGCAGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTGCGCTCGATACAGGGCACCTGTACGA  >  KT2440/1118166‑1118427
                                                                                                                                  |                                                                                                                                   
aGTGGGGTCTTGAGCAGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTgg                                                                                                                                 >  6:141677/1‑135 (MQ=255)
aGTGGGGTCTTGAGCAGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTgg                                                                                                                                 >  3:298865/1‑135 (MQ=255)
 gtagggTCTTGAGCAGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGgc                                                                                                                                <  7:136325/132‑1 (MQ=255)
 gTGGGGTGTTTAGCAGTGTTGCGGAATTCGATGAGTCCTTGCTGTTATTGGCGGGTCAACATTGGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGgc                                                                                                                                <  6:208562/135‑1 (MQ=255)
 gTGGGGTCTTGAGCAGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGTGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGgc                                                                                                                                <  1:187767/135‑1 (MQ=255)
 gTGGGGTCTTGAGCAGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGgc                                                                                                                                <  2:243270/135‑1 (MQ=255)
 gTGGGGTCTTGAGCAGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGgc                                                                                                                                <  4:55189/135‑1 (MQ=255)
   ggggTCTTGAGCAGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGgcgc                                                                                                                              <  7:137042/135‑1 (MQ=255)
   ggggTCTTGAGCAGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGgcgc                                                                                                                              <  7:133130/135‑1 (MQ=255)
       tCTTGAGCAGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAAAATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTGTTTGTATTTGGTTTTCATGTCCGCCCCCGGCTGGCGCATgg                                                                                                                          >  2:304876/1‑135 (MQ=255)
               aGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTTACGGCCAGGCCGCCACCGGCGGGCGCGTTTGATTTTGTTTTTATTTCGGGGCCCGGGTGGGGCCTGGTggggggg                                                                                                                  >  7:292417/1‑132 (MQ=255)
               aGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATg                                                                                                                  <  6:82806/135‑1 (MQ=255)
               aGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCGCGTTGTATTTGCGTTTGATGTAGGCGCCCGGGGGGCGCAAGGTTCggggg                                                                                                                  >  3:174194/1‑132 (MQ=255)
               aGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTGGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATg                                                                                                                  <  1:244764/135‑1 (MQ=255)
               aGTGTTGCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATg                                                                                                                  <  1:213459/135‑1 (MQ=255)
               aGTGTGTCTGACATCGATGACTCGTTGCTGTCATTGTCGGGGCAACATTCGATGCTGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATg                                                                                                                  <  1:218276/135‑1 (MQ=255)
                    ttCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGAccat                                                                                                             >  3:62170/1‑133 (MQ=255)
                    ttCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCtt                                                                                                             >  4:250100/1‑135 (MQ=255)
                    ttCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCGCTTTGTATTTTGTTTTCATTTCGGCGCCGGGCTGGCGCCAGGTGGGGGTGTCCtt                                                                                                             >  5:299156/1‑135 (MQ=255)
                      cGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATTTCGGCGCCGGGCTGGCGCATGGTGCGGATGACCTTGt                                                                                                           >  4:9203/1‑135 (MQ=255)
                      agacagtCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGt                                                                                                           <  3:25175/129‑1 (MQ=255)
                         aCATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCa                                                                                                        <  8:30979/135‑1 (MQ=255)
                         aCATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCa                                                                                                        <  8:256551/135‑1 (MQ=255)
                         aCATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCa                                                                                                        <  5:325341/135‑1 (MQ=255)
                         aCATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCa                                                                                                        <  2:253397/135‑1 (MQ=255)
                              gATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGac                                                                                                   >  4:311105/1‑135 (MQ=255)
                              gATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCGCTTTGTATTTGCTTTTCATGTCGGCGCCGGGCTGGCGCATGGTGCGGGTGACCTTGTGCAGCGac                                                                                                   >  4:271982/1‑135 (MQ=255)
                               tggACTCCTTGCGGTCATTGTCGGGTCAAGATTCGATGAGGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGGCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGac                                                                                                   <  5:77525/132‑1 (MQ=255)
                                   cTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTa                                                                                              >  3:222420/1‑135 (MQ=255)
                                   cTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTa                                                                                              <  8:246253/135‑1 (MQ=255)
                                          cTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGtgct                                                                                         <  3:311105/133‑1 (MQ=255)
                                           tGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGc                                                                                                                       <  8:118916/102‑1 (MQ=255)
                                           tGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGc                                                                                                                       >  7:118916/1‑102 (MQ=255)
                                             tctttGTCGGGTCAACATTCGAGGAGGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGGCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCg                                                                                    <  5:205833/132‑1 (MQ=255)
                                             tCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCg                                                                                    <  5:298462/135‑1 (MQ=255)
                                             tCATTGTCGGGTAAACATGCGAGGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGGCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCg                                                                                    <  2:192222/135‑1 (MQ=255)
                                              cATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCg                                                                                    <  8:292561/134‑1 (MQ=255)
                                              cATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCg                                                                                    <  4:284533/134‑1 (MQ=255)
                                              cATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCg                                                                                    <  7:127142/134‑1 (MQ=255)
                                              cATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCg                                                                                    <  4:222420/134‑1 (MQ=255)
                                                   tCGTGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTcgcc                                                                               <  7:52514/134‑1 (MQ=255)
                                                   tCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTcgcc                                                                               <  8:228201/134‑1 (MQ=255)
                                                   tCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTcgcc                                                                               <  5:141008/134‑1 (MQ=255)
                                                   tCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTcgcc                                                                               <  2:73764/134‑1 (MQ=255)
                                                    cGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTcgccgc                                                                             <  4:168774/135‑1 (MQ=255)
                                                      ggTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCgcgc                                                                           <  8:125466/135‑1 (MQ=255)
                                                      ggTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCgcgc                                                                           <  7:52936/135‑1 (MQ=255)
                                                         cAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAgg                                                                        <  7:247694/135‑1 (MQ=255)
                                                          aaCATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTGTCGTTTTTTTTTGTTTTAATGTCGGCGCCGGGGGGGCGCATTGTGCGGATGACCATTGCCAGCGCCACCTAGTTCTGGCCCTCGCCCCGCCggg                                                                       >  6:193577/1‑135 (MQ=255)
                                                                  atgatgTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCg                                                                <  3:250100/134‑1 (MQ=255)
                                                                    gatgTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTGCAGCGACACGTACTTCTGCCCGTCGCCGCGCCGGGCCATGCGTAc                                                             >  6:25717/1‑135 (MQ=255)
                                                                         tGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGt                                                        >  8:260914/1‑135 (MQ=255)
                                                                          gACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGt                                                        >  5:157829/1‑134 (MQ=255)
                                                                           aCGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCCTGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGtt                                                       <  3:260198/134‑1 (MQ=255)
                                                                              gCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATg                                                   >  2:283009/1‑135 (MQ=255)
                                                                                   gccgccACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCt                                               >  5:309402/1‑134 (MQ=255)
                                                                                      gccACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCAc                                           <  5:202787/135‑1 (MQ=255)
                                                                                           gggCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGc                                      <  4:153684/135‑1 (MQ=255)
                                                                                           gggCGGTCGCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGc                                      <  3:205002/135‑1 (MQ=255)
                                                                                                gTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATc                                 >  7:218969/1‑135 (MQ=255)
                                                                                                        gTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTgc                          <  8:33557/134‑1 (MQ=255)
                                                                                                        gTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTgc                          <  8:263616/134‑1 (MQ=255)
                                                                                                        gTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTgc                          <  4:298865/134‑1 (MQ=255)
                                                                                                          aTTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTGCGCt                       <  6:157829/135‑1 (MQ=255)
                                                                                                                ttttCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCg                                                                                    <  6:139216/68‑1 (MQ=255)
                                                                                                                ttttCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCg                                                                                    >  5:139216/1‑68 (MQ=255)
                                                                                                                ttttCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTGCGCTCGAt                   >  8:284728/1‑133 (MQ=255)
                                                                                                                ttttCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTGCGCTCGATa                  <  6:309402/134‑1 (MQ=255)
                                                                                                                  ttCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTGCGCTCGATACAg               >  7:14542/1‑135 (MQ=255)
                                                                                                                  ttCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCGTCACCGAGCCCCTCGCATTGCGCTCGCTACAg               >  8:126438/1‑135 (MQ=255)
                                                                                                                   tCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTGCGCTCGATACa                <  4:177608/133‑1 (MQ=255)
                                                                                                                   tCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTGCGCTCGATACa                <  4:211665/133‑1 (MQ=255)
                                                                                                                   tCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTGCGCTCGATACa                <  2:105940/133‑1 (MQ=255)
                                                                                                                               cGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTGCGCTCGATACAGGGCACCTGTACGa  >  5:197892/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                                   
AGTGGGGTCTTGAGCAGTGTTTCGGACATCGATGACTCCTTGCTGTCATTGTCGGGTCAACATTCGATGATGTTGACGGCCAGGCCGCCACGGGCGGTCTCTTTGTATTTGCTTTTCATGTCGGCGCCGGTCTGGCGCATGGTGCGGATGACCTTGTCCAGCGACACGTAGTGCTGCCCGTCGCCGCGCAGGGCCATGCGTACCGCGTTGATGGCCTTCACCGAGCCCATCGCATTGCGCTCGATACAGGGCACCTGTACGA  >  KT2440/1118166‑1118427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: