Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,342,117 A→G 6.2% intergenic (‑530/+224) nicP_1 ← / ← CJ019_01258 Porin‑like protein NicP/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,342,1170AG 6.2% 163.8 / 3.8 66intergenic (‑530/+224)nicP_1/CJ019_01258Porin‑like protein NicP/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (37/22);  new base G (2/2);  total (42/24)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.60e-01

GCGATCCTCTGTGCGGGCGTGCCCCCATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCGGCTAAGATCGAGCGCTCCCTGGCGCCACGGCCGC  >  KT2440/1341983‑1342249
                                                                                                                                      |                                                                                                                                    
gcgATCCTCTGTGCGGGCGTGCCCCCATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGa                                                                                                                                      >  2:246440/1‑135 (MQ=255)
       tctGTGCGGGCGTGCCCCCATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTc                                                                                                                                <  4:81168/134‑1 (MQ=255)
            gCGGGCGTGCCCCCATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCCTCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTg                                                                                                                          >  3:80390/1‑135 (MQ=255)
            gCGGGCGTGCCCCCATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACt                                                                                                                           <  5:40592/134‑1 (MQ=255)
                   tGCCCCCATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCAt                                                                                                                      >  7:218787/1‑132 (MQ=255)
                   tGCCCCCATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCAt                                                                                                                      <  8:218787/132‑1 (MQ=255)
                   tGCCCCCATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTc                                                                                                                   >  3:266623/1‑135 (MQ=255)
                   tGCCCCCATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTc                                                                                                                   >  5:94697/1‑135 (MQ=255)
                    gCCCCCATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGCCCACTCAGACTGGCATGt                                                                                                                    >  4:149903/1‑133 (MQ=255)
                      ccccATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCCCTCAGACTGGCATGTCCAg                                                                                                                >  3:115815/1‑135 (MQ=255)
                        ccATTGCCTGCCTCGCACCTCACACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGCCCACTCAGACTGGCATGTCCAGTc                                                                                                              >  3:238830/1‑135 (MQ=255)
                        ccATTGCCTGCCTCGCACCTCAATCCGGGGACATGGTTTACAAATCAAATCGAATGAGCAGAGGTCCCATCCGCGATCAAGCGCTATCTTCAGCTCTGGCCTCGCCCGCGCCCACTCATCCTGGCTTGGCGAGTc                                                                                                              >  4:8881/1‑135 (MQ=255)
                        ccATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGCGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTc                                                                                                              >  7:39157/1‑135 (MQ=255)
                        ccATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGGCCACTCAGACTGGCATGTCCAGTc                                                                                                              >  8:130518/1‑135 (MQ=255)
                        ccATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGGCCACTCAGACTGGCATGTCCAGTc                                                                                                              >  8:134477/1‑135 (MQ=255)
                        ccATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTc                                                                                                              >  4:28597/1‑135 (MQ=255)
                        ccATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTc                                                                                                              >  8:225309/1‑135 (MQ=255)
                        ccATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTc                                                                                                              >  5:82941/1‑135 (MQ=255)
                        ccATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTc                                                                                                              >  3:169095/1‑135 (MQ=255)
                             gcctgcctCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGc                                                                                                         >  6:310953/1‑135 (MQ=255)
                                  cctCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCt                                                                                                    <  6:216305/135‑1 (MQ=255)
                                    tCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCg                                                                                                  <  6:176728/135‑1 (MQ=255)
                                     cGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGt                                                                                                 >  6:304006/1‑135 (MQ=255)
                                        aCCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTgg                                                                                              <  7:225309/135‑1 (MQ=255)
                                               aCCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTc                                                                                                              >  1:233066/1‑112 (MQ=255)
                                               aCCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTc                                                                                                              <  2:233066/112‑1 (MQ=255)
                                                      aCATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCcctcc                                                                                <  5:310953/135‑1 (MQ=255)
                                                       cATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCcctcct                                                                               >  2:313309/1‑135 (MQ=255)
                                                            tttACAAATCAAATCGACTCAGCCTTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATTTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTc                                                                            <  2:90148/133‑1 (MQ=255)
                                                                caaatcaaatCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTg                                                                      <  5:132257/135‑1 (MQ=255)
                                                                  aatcaaatcGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCACTCGACAACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCAGTACCCCTCCTGTCGGCGTggg                                                                    >  2:59411/1‑135 (MQ=255)
                                                                   atcaaatcGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGTCTGGAATGTCCAGTCCCGGCATCCTGGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGc                                                                   <  3:244477/135‑1 (MQ=255)
                                                                           gAATCAGCAGTGGTCCCATACGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGACGGCGTGGGCGACGCctc                                                           >  8:241444/1‑135 (MQ=255)
                                                                                          ccATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTGTTACTTTTACGCAAc                                            >  1:119623/1‑135 (MQ=255)
                                                                                            aTCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGTCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGc                                          <  2:294563/135‑1 (MQ=255)
                                                                                            aTCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGc                                          <  8:195568/135‑1 (MQ=255)
                                                                                            aTCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGc                                          <  7:126008/135‑1 (MQ=255)
                                                                                            aTCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGc                                          <  7:12414/135‑1 (MQ=255)
                                                                                               cgcgAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTc                                                                            <  8:237136/98‑1 (MQ=255)
                                                                                               cgcgAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTc                                                                            >  7:237136/1‑98 (MQ=255)
                                                                                               cgcgAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGCGGCCTCGTCCTTTTCCGCCACGCATc                                       >  4:175744/1‑135 (MQ=255)
                                                                                               cgcgAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCCACGcgtg                                       >  7:321964/1‑132 (MQ=255)
                                                                                               cgcgAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCGTCCTTTTCCGCCACGCTTc                                       >  1:245165/1‑135 (MQ=255)
                                                                                                  gAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTACGCAACGCGTCCCg                                    >  5:314961/1‑135 (MQ=255)
                                                                                                   aGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGGCCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCgg                                   <  7:130518/135‑1 (MQ=255)
                                                                                                   aGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGGCCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCgg                                   <  7:134477/135‑1 (MQ=255)
                                                                                                    gCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGCCTGGCATGTCCAGTCCCGGCATCCTCGTAGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCGGTTGCGCACCGCTTCCCgg                                   >  3:200982/1‑134 (MQ=21)
                                                                                                    gCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCgg                                   >  7:61060/1‑134 (MQ=255)
                                                                                                    gCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCgg                                   >  7:24142/1‑134 (MQ=255)
                                                                                                           cTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCGGCTAAGATc                           <  4:61228/135‑1 (MQ=255)
                                                                                                           cTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCGGCTAAGATc                           <  6:82941/135‑1 (MQ=255)
                                                                                                               cTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCGGCTAAGATCGa                         <  2:281177/133‑1 (MQ=255)
                                                                                                               cTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCGGCTAAGATCGa                         <  3:321448/133‑1 (MQ=255)
                                                                                                                 tCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCGCTTCCGTTTCCGCAACGCTTCCCGGGGAAGATCgcgcgc                     >  8:125906/1‑135 (MQ=21)
                                                                                                                     aTCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCGTCCGTTTCCGCAACGCGTCCCGGCTAAGATCGAGCGCTccc                 >  5:106150/1‑135 (MQ=255)
                                                                                                                      tCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTACGCAACGCTTCCCGGCTAAGATCGAGCGCTCCCt                >  6:9389/1‑135 (MQ=255)
                                                                                                                      tCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCGTTTCCGCAACGCTGACCGGCTAAGATCGAGAGCTCCCt                >  6:13705/1‑135 (MQ=255)
                                                                                                                           ccTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCGGCTAAGATCGAGCGCTCCCTGgcgc           >  6:128227/1‑135 (MQ=255)
                                                                                                                           ccTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCGCTTCCTTTTACGCCAAGCTTCCCGGCTAAGATCGAGCGCTCCCTGgcgc           >  8:300122/1‑135 (MQ=255)
                                                                                                                             tCGCCCTCGACCGCTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGCGGCCGCGTCCGTTTCCGCCACGCGTCCCGGCGAAGATCGCGCGCTCCCGGGCGCCa         >  2:267398/1‑135 (MQ=21)
                                                                                                                             tCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTg                                                                                            <  5:231745/52‑1 (MQ=255)
                                                                                                                             tCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTg                                                                                            >  6:231745/1‑52 (MQ=255)
                                                                                                                             tCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGCGGCCTCGGCCGTTTCCGCAACGCGTCCCGGCGAACGACCCGCGCTCCCAgccgccc         >  6:204454/1‑134 (MQ=18)
                                                                                                                             tCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCGGCTAAGATCGCGCGCTCCCTGGCGCCa         >  8:270604/1‑135 (MQ=255)
                                                                                                                             tCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCGCTTCCTTTTCCGCAACGCGTCCCGGCTAAGATCGAGCGCTCCCTGGCGccc         >  8:165538/1‑134 (MQ=255)
                                                                                                                                cccTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCGGCTAAGATCGAGCGCTCCCTGGCGCCAc        <  1:246440/133‑1 (MQ=255)
                                                                                                                                  cTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCGGCTAAGATCGAGCGCTCCCTGGCGCCACGgcc    >  6:120004/1‑135 (MQ=255)
                                                                                                                                      aCCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCGGCTAAGATCGAGCGCTCCCTGGCGCCACGgccgc  <  2:318471/133‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
GCGATCCTCTGTGCGGGCGTGCCCCCATTGCCTGCCTCGCACCTCAAACCGGGGACATGGTTTACAAATCAAATCGAATCAGCAGTGGTCCCATCCGCGAGCATGCGCTATCTTCAGATCTGGCCTCGCCCTCGACCACTCAGACTGGCATGTCCAGTCCCGGCATCCTCGTTGGTGCCGTACCCCTCCTGTCGGCGTGGGCGAGGCCTCTTCCTTTTCCGCAACGCTTCCCGGCTAAGATCGAGCGCTCCCTGGCGCCACGGCCGC  >  KT2440/1341983‑1342249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑