Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,362,136	C→T	9.0%	R241W (CGG→TGG)	CJ019_01276 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,362,136	0	C	T	9.0%	167.3 / 5.4	55	R241W (CGG→TGG)	CJ019_01276	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/28);  new base T (2/3);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TGCACAACCTCAGCGGCGAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCTTTATTTCTACACTGAAGCTACCGATTCCGATCCGTCGAAGACACCCGGCCCATGACTCTTCCT  >  KT2440/1362002‑1362269                                                                                                                                       |                                                                                                                                      tGCACAACCTCAGCGGCGAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGcc                                                                                                                                       >  7:218096/1‑135 (MQ=255)    acaACCTCAGCGGCGAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGc                                                                                                                                    >  2:222248/1‑135 (MQ=255)      aaCCTCAGCGGCGAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCtt                                                                                                                                  >  8:298790/1‑135 (MQ=255)       aCCTCAGCGGCGAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTg                                                                                                                                 <  8:49024/135‑1 (MQ=255)       aCCTCAGCGGCGAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTg                                                                                                                                 <  6:41951/135‑1 (MQ=255)       aCCTCAGCGGCGAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTg                                                                                                                                 <  7:131348/135‑1 (MQ=255)       aCCTCAGCGGCGAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTg                                                                                                                                 <  3:247023/135‑1 (MQ=255)       aCCTCAGCGGCGAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTg                                                                                                                                 <  3:15857/135‑1 (MQ=255)       aCCTCAGCGGCGAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTg                                                                                                                                 <  7:273081/135‑1 (MQ=255)       aCCTCAGCGGCGAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTg                                                                                                                                 <  1:19763/135‑1 (MQ=255)       aCCTCAGCGGCGAAGGCCCGCCATGTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTg                                                                                                                                 <  6:329694/135‑1 (MQ=255)              cggcgAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCagcagc                                                                                                                          >  4:128847/1‑135 (MQ=255)              cggcgAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCagcagc                                                                                                                          >  2:289631/1‑135 (MQ=255)                gcgAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGt                                                                                                                        >  6:96506/1‑135 (MQ=255)                 cgAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGtt                                                                                                                       <  7:106208/135‑1 (MQ=255)                 cgAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGtt                                                                                                                       <  1:222248/135‑1 (MQ=255)                 cgAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGtt                                                                                                                       <  3:113419/135‑1 (MQ=255)                 cgAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGtt                                                                                                                       <  8:326719/135‑1 (MQ=255)                      gCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGgaga                                                                                                                  >  1:288811/1‑135 (MQ=255)                           ccATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCt                                                                                                             <  3:128847/135‑1 (MQ=255)                              ttttttGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCtga                                                                                                           >  3:276000/1‑134 (MQ=255)                              ttttttGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCtga                                                                                                           >  3:207737/1‑134 (MQ=255)                                 tttGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCtgatga                                                                                                        <  6:339589/134‑1 (MQ=255)                                        gtagcgtGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTa                                                                                                >  7:21435/1‑135 (MQ=255)                                            cgtGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCg                                                                                             <  4:264089/134‑1 (MQ=255)                                                cACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGGGAGATGTGGTAGCCAAGGAGGGCATGCAGACACTACTTGCCTTCCGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGaacaa                                                                                        <  8:173240/135‑1 (MQ=255)                                                cACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGaacaa                                                                                        <  6:32604/135‑1 (MQ=255)                                                   tACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGt                                                                                     <  1:289631/135‑1 (MQ=255)                                                        tGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGCTGAGCCCGGTATCGACCACCGGCAgc                                                                                 >  2:258723/1‑134 (MQ=255)                                                        tGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAgcg                                                                                <  1:97096/135‑1 (MQ=255)                                                        tGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAgc                                                                                 >  7:244219/1‑134 (MQ=255)                                                           cTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCa                                                                              >  1:98436/1‑134 (MQ=255)                                                           cTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCa                                                                              >  7:149775/1‑134 (MQ=255)                                                           cTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCa                                                                              >  7:137417/1‑134 (MQ=255)                                                              gAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCAc                                                                          >  5:81788/1‑135 (MQ=255)                                                                  ccGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTc                                                                      >  7:329321/1‑135 (MQ=255)                                                                  ccGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTc                                                                      >  6:116676/1‑135 (MQ=255)                                                                  ccGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCCGATGAGCCCGGTATCGAACAACGTCAGCGCATCCCTGTc                                                                      >  2:87374/1‑135 (MQ=255)                                                                     gCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGGTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCggg                                                                   <  1:161519/135‑1 (MQ=255)                                                                       cAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCTGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCt                                                                 >  3:208709/1‑135 (MQ=255)                                                                       cAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCTGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCt                                                                 >  3:212938/1‑135 (MQ=255)                                                                       cAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCt                                                                 >  1:116854/1‑135 (MQ=255)                                                                        aGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCtt                                                                <  2:288811/135‑1 (MQ=255)                                                                           gTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCACCACTGTCGGGCTTTAt                                                             <  4:220343/135‑1 (MQ=255)                                                                             cGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCTTTAttt                                                           >  7:132228/1‑135 (MQ=255)                                                                                  aTTTGCGAGATGTGGGAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGAGGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCTTTATTTCTaca                                                      <  2:136899/135‑1 (MQ=255)                                                                                         agaTGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCTTTATTTCTACACTGAAGc                                               <  8:329321/135‑1 (MQ=255)                                                                                                    ccAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCTTTATTTCTACACTGAAGCTACCGATTcc                                     <  2:116854/134‑1 (MQ=255)                                                                                                                 gCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCTTTATTTCTACACTGAAGCTACCGATTCCGATCCGTCGAAGac                       <  4:276000/135‑1 (MQ=255)                                                                                                                 gCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTGGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCTTTATTTCTACACTGAAGCTACCGATTCCGATCCGTCGAAGac                       <  5:96506/135‑1 (MQ=255)                                                                                                                  ctgACAGTACTTGCTTTCAGCTGGCTTGCAGCAGGGTTGGAGTGTGCTGTTGAGCCTGTTATCGAACAACGTAAGCGCATCACTTTCGGGCTTTATTTCTACACTGAAGCTACCGATTCCGATCCGTCGAAGac                       <  4:207737/132‑1 (MQ=255)                                                                                                                     acacTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCTTTATTTCTACACTGAAGCTACCGATTCCGATCCGTCGAAGACAccc                   >  4:310302/1‑135 (MQ=255)                                                                                                                         tactTGCCTTCAGCTGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCTTTATTTCTACACTGAAGCTACCGATTCCGATCCGTCGAAGACACCCGGcc               <  4:212938/135‑1 (MQ=255)                                                                                                                         tactTGCCTTCAGCTGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCTTTATTTCTACACTGAAGCTACCGATTCCGATCCGTCGAAGACACCCGGcc               <  4:208709/135‑1 (MQ=255)                                                                                                                            ttGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCTTTATTTCTACACTGAAGCTACCGATTCCGATCCGTCGAAGACACCCGGCCCa             <  8:21435/134‑1 (MQ=255)                                                                                                                                    aGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGTGCTTTATTTCTACACTGAAGCTACCGCTTCCGATCCGTCGAAGACACCCGGACCATGACTCTTc    >  8:214253/1‑135 (MQ=255)                                                                                                                                      ccGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCGCGGTATCGAACAACGTCAGCGCATCACTGTCGGGGGTTATTTCTACACTGAACCTACCGATTCCGATCCGTCGAAGACACCCGGGCCATGCAGCTTCCt  >  5:237561/1‑135 (MQ=255)                                                                                                                                      ccGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATc                                                                                              >  2:225417/1‑43 (MQ=255)                                                                                                                                      ccGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATc                                                                                              <  1:225417/43‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      TGCACAACCTCAGCGGCGAAGGCCCGCCATTTTTTGAGCGTAGCGTGCACTACGATGCCTTGAGCCCGGCCAGCGTCGAGCATTTGCGAGATGTGGTAGCCAAGGATGGCATGCAGACACTACTTGCCTTCAGCCGGCTTGCAGCAGCGTTGGAGAGTGCTGATGAGCCCGGTATCGAACAACGTCAGCGCATCACTGTCGGGCTTTATTTCTACACTGAAGCTACCGATTCCGATCCGTCGAAGACACCCGGCCCATGACTCTTCCT  >  KT2440/1362002‑1362269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑