Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,422,128 G→C 12.7% W410S (TGG→TCG)  mqo_2 → Malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,422,1280GC12.7% 140.1 / 3.6 57W410S (TGG→TCG) mqo_2Malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (29/20);  new base C (2/6);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATCGCGGGCCTGCTGGGTGCATCGC  >  KT2440/1421995‑1422259
                                                                                                                                     |                                                                                                                                   
gtgtGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGACCGCCAAGAAAGAAGAATgg                                                                                                                                    >  6:114950/1‑135 (MQ=255)
gtgtGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGAGGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATgg                                                                                                                                    <  1:12284/135‑1 (MQ=255)
     gCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCt                                                                                                                               >  4:209154/1‑135 (MQ=255)
      ccGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCCCCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCtg                                                                                                                              >  5:275949/1‑135 (MQ=255)
      ccGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCtg                                                                                                                              >  5:282806/1‑135 (MQ=255)
          tGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCtgtg                                                                                                                            >  7:132336/1‑133 (MQ=255)
          tGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCCCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGc                                                                                                                          >  8:320294/1‑135 (MQ=255)
            aCCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAg                                                                                                                        >  1:189383/1‑135 (MQ=255)
              cAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAggc                                                                                                                      >  8:190600/1‑135 (MQ=255)
                   tCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCGGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAACAAAGAAGAATGGCGCCTGTGGCAggccggcc                                                                                                                 <  3:225267/135‑1 (MQ=255)
                   tCGGTATCGACCAGACCCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCAACTTCCCGAACGCCAAGAAACAAGAATCGCGCCTGTGGCAggccggcc                                                                                                                 <  4:112512/135‑1 (MQ=38)
                       tATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAgcg                                                                                                             >  5:149323/1‑135 (MQ=255)
                           gcccAGTACCCACTGGTCGAGTACCGCGCTGGCCAGCTGATGCTGTCGCACGATGACCGCTGCGACCCCCTGCCCACATACTCCCCGAACGCCAAGAAAGACGATTCGCGCCTGTGGCAGGCCGGCCAGCGCGTg                                                                                                         <  2:119643/133‑1 (MQ=255)
                           gACCAGTACCCCCTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGTTGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTg                                                                                                         <  1:297094/135‑1 (MQ=255)
                           gACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCCAACGCCAACAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTg                                                                                                         <  5:260833/135‑1 (MQ=255)
                               aGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCaaa                                                                                                     >  5:145588/1‑135 (MQ=255)
                                    ccACTGGTCGAGTACCTCGCTGGCCAGCTGCTGCTGTCTGACGATGCCCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATCGCGCCTGTCGCCGGCCGGCCAGCGCGTGCAAatcat                                                                                                 >  7:214509/1‑134 (MQ=25)
                                    ccACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcat                                                                                                 >  2:30995/1‑134 (MQ=255)
                                        tggtggAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCGGCGCACCTACTTCCCGAACGCCAAGAACGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAAc                                                                                            <  6:255179/135‑1 (MQ=255)
                                         ggTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCaaa                                                                                             >  1:323749/1‑133 (MQ=255)
                                             gAGTACCTCGCTGTCCTGATGATGCTGTCTGACGAGGACCGCTGCGACGCCCTGCGCACCTACATCCCGAACGCCAAGAAAGAAGATTGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGAt                                                                                       <  8:22304/135‑1 (MQ=255)
                                              aGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGACCCCCAAGAAAGAAGAATGCCGCGTGTGCCCGGCCGGCCAGCGCGTGCAAATCTACAAACGTCATg                                                                                      >  3:139568/1‑135 (MQ=25)
                                              aGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATg                                                                                      >  7:133931/1‑135 (MQ=255)
                                                 aCCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGcc                                                                                    <  6:149323/134‑1 (MQ=255)
                                                           ccAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGggcgg                                                                         >  2:99397/1‑135 (MQ=255)
                                                             aGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGgcggcg                                                                       >  4:171723/1‑135 (MQ=255)
                                                                tGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGc                                                                    >  5:284408/1‑135 (MQ=255)
                                                                     cTGTCTGACCAGGACCGCCTCGAAGCCCTGCGCACCAACTTCCCGACCGCCACGAAACAAGAATCGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGaa                                                                <  5:319698/134‑1 (MQ=255)
                                                                      tGTCTGACGATGCCCGCTGCGAAGCCCTGCGAACCTACTTCCCTAACCCAAACAAAGAATAATCGCGCCGGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGaa                                                                <  1:207100/133‑1 (MQ=255)
                                                                         cTGACGATGACGGGTTCGCCGCCCGGGGCACCTACTTCCCTAACGGCTAGAAAGAAGACTGGCGCGTGTGGCAGGCCGGCGAGGGCGTGCAAATCATCAAAGGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTgg                                                           <  6:80002/135‑1 (MQ=255)
                                                                         cTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCt                                                             <  6:145588/133‑1 (MQ=255)
                                                                                   ccGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAgt                                                 <  8:132336/135‑1 (MQ=255)
                                                                                     ggttCGAAGCCAGGCGAACCTTCTACCCGACCCCCCTCAAAGAAGATGGGCGCCTGTTGCAGGCCGGCCAGCGCGTGAAAATCATGTAAGGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTTAAgtgg                                               <  5:282820/133‑1 (MQ=255)
                                                                                     gCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAgtgg                                               >  1:229383/1‑135 (MQ=255)
                                                                                     gCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAgtgg                                               >  5:239223/1‑135 (MQ=255)
                                                                                     gCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGAAGCAGAGAAGGGCGGCGTGCTGAATCTGCGCACTCAAgtgg                                               >  2:25681/1‑135 (MQ=255)
                                                                                     gCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCAACGACAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCACATCATCAAACGTTATGCCGAGAAGGGCGGCGTGCTGAAGCTGTGCACTGAAgtgg                                               <  3:150432/135‑1 (MQ=255)
                                                                                                    gcACCTACTTCCCGACCCCCAAGAAACAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGAcc                                <  3:27365/135‑1 (MQ=255)
                                                                                                    gcACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGTCGTCCGAAGAcc                                >  8:45273/1‑135 (MQ=255)
                                                                                                    gcACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGAcc                                >  4:90836/1‑135 (MQ=255)
                                                                                                    gcACCTACTCCCCGCACGCCAAGAAAGAACAATCCCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGGGAGGCCTCGAAGGGCGGCGTGCGCAAGCTGGGCACCCAAGTTGGGGCGTCCCAAGccc                                >  5:209335/1‑135 (MQ=255)
                                                                                                      aCCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGc                              >  1:316629/1‑135 (MQ=255)
                                                                                                            ttCCCGACCCCCACAAAACAACACTCGCGCCTGTGGCAGGCCGGCCAGCGCGTGCATATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATc                        <  5:274470/135‑1 (MQ=255)
                                                                                                            ttCCCGAACGCCAAGAAAGAAGATTGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCa                          <  7:183252/133‑1 (MQ=255)
                                                                                                            taccccAACGCCCCCAACGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCa                          <  5:36449/131‑1 (MQ=255)
                                                                                                             tCCCGAACGCCAAGAAACAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATcg                       <  4:139568/135‑1 (MQ=255)
                                                                                                              cccGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTg                                                                                                         >  7:84183/1‑52 (MQ=255)
                                                                                                              cccGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTg                                                                                                         <  8:84183/52‑1 (MQ=255)
                                                                                                                cGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATCGCgg                    >  7:219132/1‑135 (MQ=255)
                                                                                                                cGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATCGCgg                    >  5:51601/1‑135 (MQ=255)
                                                                                                                    ccccAACACAGAAGAAGCGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATCGCGGGCct                <  2:316629/133‑1 (MQ=255)
                                                                                                                    cGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATCGCGGGCct                <  7:33275/135‑1 (MQ=255)
                                                                                                                     gCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATCGCGGGCctg               >  2:59606/1‑135 (MQ=255)
                                                                                                                      ccAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATCGCGGGCctgc              <  6:166042/135‑1 (MQ=255)
                                                                                                                           aaagaaGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATCGCGGGCCTGCTGGGt         <  7:41498/135‑1 (MQ=255)
                                                                                                                            aagaagAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATCGCGGGCCTGCTGGGt         >  1:236851/1‑134 (MQ=255)
                                                                                                                                  aaTGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCCCCATCGCGGGCCTGCTGGGTGCATCGc  >  5:248999/1‑135 (MQ=255)
                                                                                                                                  aaTGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATCGCGGGCCTGCTGGGTGCATCGc  <  5:257894/135‑1 (MQ=255)
                                                                                                                                  aaTGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATCGCGGGCCTGCTGGGTGCATCGc  >  4:14274/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                   
GTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCCGAGAAGGGCGGCGTGCTGAAGCTGGGCACTGAAGTGGTGGCGTCCGAAGACCGCACCATCGCGGGCCTGCTGGGTGCATCGC  >  KT2440/1421995‑1422259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑