| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,670,696 | A→C | 44.2% | intergenic (+33/‑98) | thrC_2 → / → CJ019_01569 | Threonine synthase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,670,696 | 0 | A | C | 44.2% | 13.4 / 11.1 | 37 | intergenic (+33/‑98) | thrC_2/CJ019_01569 | Threonine synthase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (15/5); new base C (12/5); total (27/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCCCTATGTGTTGATTCACC > KT2440/1670571‑1670821 | aGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCCGCGCAGCCCCAACGTCTCAg > 7:7186/1‑135 (MQ=255) agagCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTcc > 6:130767/1‑135 (MQ=255) tGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCAGCCCCAACGTCTCCGCCGTTTGCCTCCTGt > 7:212081/1‑135 (MQ=255) ccTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCCGCGCAGCCCCCACCTCTCCGCCGTGTCCCTCCTGTCTATccg > 6:281394/1‑132 (MQ=255) cgggCCAACGCCCTGACGGCTGTACTGGGCTTTGCCACCCAGCACGCAACCCGCGGCAAGCCGCTTTAATTGCAGGGGGTGT‑GGGGCTGCAGCGCAGCCCCCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGaa < 4:253124/133‑1 (MQ=255) ccAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCCGCGCCGCCCCAACGTCGCAGCCGTTTCCCGCCTGTCTATCAGAAACAt > 6:279723/1‑135 (MQ=255) accGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTTCAGCGCAGCCCCAACATCTCAGCCTTTTGCCTCCTTTGTATAATCAACATcc > 6:238270/3‑135 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑TGGGCGGCCGCGCAGCCCCCACGGCGCAGCCGTTTACCGGCTTTCGATCAGAAACATACTATTc > 8:122542/1‑135 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑TGGGCGGCCGCGCACCCCCCAAGGCTACGCCTTTTCCCACCAGTCGATCACAAACATCCTaat > 2:185601/1‑132 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGGAGCGCAGCCCCCACGTCGCACCCTTTTGCCGCCGGTCTATCAGAAACATCCTAt > 5:104602/1‑133 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCCGCGCCCCCCCAACCTCGCAGCCATTTACCTCCGGTCTATCAGAAACagacaat > 5:62552/1‑127 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCCGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTAt > 7:281254/1‑133 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCCGCCCCCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTAt > 4:225444/1‑133 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCCGCCCCAACGTCTCAGCCGTTTCCCTCCTGTCTATCAGAAACATCCTATTc > 2:275952/1‑135 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCAGCCCCCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTAtt > 6:280491/1‑134 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCAGCCCCAACGTCTCCGCCTTTTCCCTCCTGTCTATCAGAAACATCCTAt > 6:141775/1‑133 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTACTGTCTATCAGAAACATCCTAt > 8:185001/1‑133 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCCCAGCCCCCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTc > 4:232330/1‑135 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCGGGCCCGCAGCCCCAACGCACCAACCGTTTACCTCATGTCGATAAGTAACAACCTAATc > 1:258737/1‑135 (MQ=255) aCAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGgc > 6:321980/1‑135 (MQ=255) cAGGGCTTTGTCCGCCTGCACGGAACCCGCGGCAAGCCGCTGTAATTGCAGGGGGTTT‑GGGGGTGCACGGCAGCCCAAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGgc < 3:170105/134‑1 (MQ=255) aGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGAGTTT‑GGGGGTGGCGCGCCGCCCCCACGGCTCCGCCGTTTGCCGCCGGGGTATCAGCAACATCCGATTAAGCCTGCCGGgcgc > 8:102163/1‑135 (MQ=255) tGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCCGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCa > 7:258167/1‑134 (MQ=255) tGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCAGCCCCAACGGCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCa > 2:10257/1‑134 (MQ=255) cagccagcACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGGAGCAGCGCCGCCCCCACCGCTCAGCCTTTTCCCTCCGGTCGATCAGAAACATCCTATTCAGCCTGCCCGGCGCCAAGCATaa > 6:91641/1‑134 (MQ=255) cagccagcACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCCGCGCAGCCCCCACGGCGCAGCCTTTTCCCGCCTGTCTATCAGAAACATCCTATTCCGCCTGCCGGGCGCCCAGCCTaaa > 2:98044/1‑135 (MQ=255) cagccagcACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCCACCCCCACCTCTCAGCCTTTTACCGCCGGTCGTTCCTCAACATCCTATTCAGCCTGCCCGGCGCCCAGcgataa > 5:268620/1‑130 (MQ=255) cagccagcACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCAGCCCCAACGTCGCAGCCGTTTCCCTGCTGTCGATAATAAACATTCTATTCCGCCTGCCGGGCGCCAAGCATaaa > 2:259761/1‑135 (MQ=255) cgcaaaCCGCGGCAAGCCGCTGAATTTGCTGGGCGTTG‑GGGGCGGCCGCGCACCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGt < 7:10128/131‑1 (MQ=255) ggCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCAGCCCCCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTAATCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGtc > 7:119471/1‑135 (MQ=255) cAACCCGCTGTATTTGCAGGGCGTTG‑GGGGGGCCCGCGCACCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAgg < 4:274665/135‑1 (MQ=255) ttGCATGGCGTTT‑GGGGGGCCCGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGc < 2:21952/134‑1 (MQ=255) atGCTTGGGGTTTGGGGGCTGCCGCCCCCCCCCCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAACATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATg < 6:290061/133‑1 (MQ=255) ggCGGTG‑GGGGCGGCCGCGCAGCCCCCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGcc < 2:134668/135‑1 (MQ=255) gcagcccagcCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCCCTATGTGTTGAtt < 3:234455/135‑1 (MQ=255) cgccgcCCACACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCCCTATGTGTTGATTCAcc < 7:185001/135‑1 (MQ=255) cccccccccCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCCCTATGTGTTGATTCAcc < 5:209272/130‑1 (MQ=255) | AGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTT‑GGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCCCTATGTGTTGATTCACC > KT2440/1670571‑1670821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |