| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,720,958 | T→G | 37.4% | T88P (ACC→CCC) | plsB ← | Glycerol‑3‑phosphate acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,720,958 | 0 | T | G | 37.4% | 22.4 / 2.5 | 36 | T88P (ACC→CCC) | plsB | Glycerol‑3‑phosphate acyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (13/8); new base G (6/7); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGCAACGCATAGA > KT2440/1720834‑1721092 | aaCACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTGTAGTCCTGGCCGCCGCGGCCGGCCAGGTCGGGGGTAAgggtaga > 1:314344/1‑129 (MQ=255) acacGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGGCGGGGGTCAGGTtgaaga > 5:94031/1‑135 (MQ=255) acacGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCGGGCGGCCGCGCCAGTCCGGGTCGGGGGTCAGGTagaaga > 6:220998/1‑135 (MQ=255) gtGACAGGAATGATCTGCGCATCTTCTGCGGCAGGCGGGCTGAGGGCAGCCACCCGGCGCTCGAGGGGGGGGGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGGGGGGGGTCTGGTAGAAGAAcgcg < 6:94031/134‑1 (MQ=255) cTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAAcgcg > 8:276834/1‑135 (MQ=255) cTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAAcgcg < 8:270085/135‑1 (MQ=255) gACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCCAGCCAGTCCGGGTCGGGGGGCAGGTATAACAAcgccgc > 5:284735/1‑135 (MQ=255) gACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTGTGCGGGCGGCCGCGCCCGGGCGCGTCGGGGGGCCGGTTGGAGACCgcggc > 5:239163/1‑135 (MQ=255) aCAGGAATGATCTGCGCATCTGCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGGCAGGTAGAAGAACgcggcg < 8:41129/135‑1 (MQ=255) gAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGtt > 8:64972/1‑135 (MQ=255) gcATCTTCTTCGGCATGCTGGCTGACGGCAGCCCCCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTGTCCGGGCGGGCGCGCCAGTCCGGGGCGGGGGGGAGGTAGAAGCAACCGGCGGGTTGCTGCAGTggg > 2:135240/1‑135 (MQ=255) cATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTTTCCGTGCGGGCGCGGCCGGCCGGGGCCGGGGTGGGGGAGAAGACCGCGGCGGGTTCCTGCAGTGGGc > 1:156535/1‑135 (MQ=255) aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGCGCCAGGGCGGGTCGGGGGGCAGGTAGAAGAACGCGGCGGGGTCCGGCAGTGGGcc > 5:24759/1‑135 (MQ=255) aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGcc > 6:122557/1‑135 (MQ=255) aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGcc > 1:198134/1‑135 (MQ=255) aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTTTCCTGGCGGCCGAGCCAGTCCGGGGCGGGGGTGAGGGTGAAGAACGCGGCGGGGTCCTGCCGTggggc > 5:20473/1‑133 (MQ=255) ttcGGCCGGCTGGCGGCCGGCAGCCACCCGGCGCTCGAGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGGCCGGGGCGGGGGGCAGGTAGACGACCGCGGCGGGTTCCTGCAGTGGGCCGACg < 4:71721/133‑1 (MQ=255) ttcGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGGCCGGGCGGCCGAGCCAGTCCGGGGCGGGGGGCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACg < 8:11821/133‑1 (MQ=255) ttcGGCATGCTGGCGGACGGCACCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACg < 4:309944/133‑1 (MQ=255) ggCTTGCGGGCTGCCGGCGGCCACCCGGCGCTGGCGCGGGGGCGGGGGGCCGCTTTTGTCTGGGCGGCCGAGCCAGTCCGGGGCGGGGGTCGGTTAGAAGCCCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTAc < 1:217952/135‑1 (MQ=255) gCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTGTGCTGGGGGCCGAGCCAGTCCGGGTCGGGGGGGAGGTAGCATCACGCGGCGGGTTCCTGGAGTGGGGCGCCGGCGAcc > 2:143418/1‑135 (MQ=255) gCAGGCGGGCTCACGCCCCCCACCCGGCGCGGGCGCGGGGGCGGGGCGCCGCTTTTGCCCTGGCGGCCGAGCCAGTCGGGGGCGGGGGTCCGTTACAACAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTAcc < 5:122557/135‑1 (MQ=255) ggctgACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTGTCCTGGCGGGCGGGCCAGTGCGGGTGGGGGGGGAGGTTGAAGAACGCGGCGGGTTGCTGCAGTGGGCCGGCGGCTACCGgcagca > 1:303014/1‑135 (MQ=255) ggctgACGGCAGCCACAAGGCTCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCAGGGTCGGGGGTCAGGTATAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGGTACCGgccgca > 6:18947/1‑135 (MQ=255) gAGCGTGGGCGGGGCGCCGCTTTTGTCCTTGCGGCCGAGCCAGTCAGGGTCGGGGGTCAGGTAGAATAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGt > 5:5329/1‑135 (MQ=255) ggggggggggcgCGGTTTTGGCCCGGGGGGCCGCGCCAGGCGGGGGGGGGGGTCTGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCAc < 5:220998/133‑1 (MQ=255) gggggcggggcgCCGCTTTTGTCCTGGCGGCCGAGCCAGGCCGGGGCGGGGGGCAGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCAc < 7:193594/133‑1 (MQ=255) gccgcTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGCCGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCGTGGTGCACTCGTGGTCGAg > 1:110654/1‑135 (MQ=255) ccgcTTTTGTCCAGGCGGCCGCGCCAGTCCGGGTCGGGGGTCAGGTATAAGAAAGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCCGCAGCACCCGGCGCGGCCGCCCCCCCATGGGGCCCTCCGGGTcccgc > 6:254157/1‑131 (MQ=255) cTTTGGGCCGGGCGCCCGCGCCAGGCGGGGGGGGGGGGCTGGTACAACCCCGCGGCGGGTGCCGGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTGGGTGCACTCGTGGTCGAGCACg < 4:195008/135‑1 (MQ=255) cgggcggcCGAGCCAGGCCGGGGGGGGGGGCAGGTAGAAGACCGCGGCGGGGTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTc < 2:198134/133‑1 (MQ=255) ggcCGCGCCTGGCGGGGGCGGGGGCCTGGTCGCACCCCGCGGCGGGTGCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGGCGAGCACGGCCAGGTCGGTGa < 1:225273/135‑1 (MQ=255) ccgggggCGGGGGCCAGGTACACCCCCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAg < 3:221288/132‑1 (MQ=255) ccGGGGCGGGGGGCAGGTCGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGAGCGAgg < 2:218722/135‑1 (MQ=255) ggggCGGGGGTCCGGGAGAAACCCGCGGCGGGTGCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTGGGTGCACTCGGGGGCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGc < 3:151770/135‑1 (MQ=255) cGGGGGTCCGGTAGAAGAACGCGGCGGGTGCCTGCCGGGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGCAACg < 7:243848/135‑1 (MQ=255) tCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGCAACGcctgtc > 5:95877/1‑130 (MQ=255) | AACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGCAACGCATAGA > KT2440/1720834‑1721092 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |