Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,720,970 A→C 32.1% F84V (TTC→GTC)  plsB ← Glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,720,9700AC32.1% 35.2 / 6.9 35F84V (TTC→GTC) plsBGlycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (15/8);  new base C (2/10);  total (17/18)
Fisher's exact test for biased strand distribution p-value = 1.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGCAACGCATAGA  >  KT2440/1720840‑1721092
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gtGACAGGAATGATCTGCGCATCTTCTGCGGCAGGCGGGCTGAGGGCAGCCACCCGGCGCTCGAGGGGGGGGGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGGGGGGGGTCTGGTAGAAGAAcgcg                                                                                                                        <  6:94031/134‑1 (MQ=255)
cTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAAcgcg                                                                                                                        >  8:276834/1‑135 (MQ=255)
cTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAAcgcg                                                                                                                        <  8:270085/135‑1 (MQ=255)
  gACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCCAGCCAGTCCGGGTCGGGGGGCAGGTATAACAAcgccgc                                                                                                                      >  5:284735/1‑135 (MQ=255)
  gACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTGTGCGGGCGGCCGCGCCCGGGCGCGTCGGGGGGCCGGTTGGAGACCgcggc                                                                                                                      >  5:239163/1‑135 (MQ=255)
   aCAGGAATGATCTGCGCATCTGCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGGCAGGTAGAAGAACgcggcg                                                                                                                     <  8:41129/135‑1 (MQ=255)
       gAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGtt                                                                                                                 >  8:64972/1‑135 (MQ=255)
                  gcATCTTCTTCGGCATGCTGGCTGACGGCAGCCCCCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTGTCCGGGCGGGCGCGCCAGTCCGGGGCGGGGGGGAGGTAGAAGCAACCGGCGGGTTGCTGCAGTggg                                                                                                      >  2:135240/1‑135 (MQ=255)
                   cATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTTTCCGTGCGGGCGCGGCCGGCCGGGGCCGGGGTGGGGGAGAAGACCGCGGCGGGTTCCTGCAGTGGGc                                                                                                     >  1:156535/1‑135 (MQ=255)
                    aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGCGCCAGGGCGGGTCGGGGGGCAGGTAGAAGAACGCGGCGGGGTCCGGCAGTGGGcc                                                                                                    >  5:24759/1‑135 (MQ=255)
                    aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGcc                                                                                                    >  1:198134/1‑135 (MQ=255)
                    aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGcc                                                                                                    >  6:122557/1‑135 (MQ=255)
                    aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTTTCCTGGCGGCCGAGCCAGTCCGGGGCGGGGGTGAGGGTGAAGAACGCGGCGGGGTCCTGCCGTggggc                                                                                                    >  5:20473/1‑133 (MQ=255)
                          ttcGGCCGGCTGGCGGCCGGCAGCCACCCGGCGCTCGAGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGGCCGGGGCGGGGGGCAGGTAGACGACCGCGGCGGGTTCCTGCAGTGGGCCGACg                                                                                                <  4:71721/133‑1 (MQ=255)
                          ttcGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGGCCGGGCGGCCGAGCCAGTCCGGGGCGGGGGGCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACg                                                                                                <  8:11821/133‑1 (MQ=255)
                          ttcGGCATGCTGGCGGACGGCACCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACg                                                                                                <  4:309944/133‑1 (MQ=255)
                             ggCTTGCGGGCTGCCGGCGGCCACCCGGCGCTGGCGCGGGGGCGGGGGGCCGCTTTTGTCTGGGCGGCCGAGCCAGTCCGGGGCGGGGGTCGGTTAGAAGCCCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTAc                                                                                           <  1:217952/135‑1 (MQ=255)
                              gCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTGTGCTGGGGGCCGAGCCAGTCCGGGTCGGGGGGGAGGTAGCATCACGCGGCGGGTTCCTGGAGTGGGGCGCCGGCGAcc                                                                                          >  2:143418/1‑135 (MQ=255)
                              gCAGGCGGGCTCACGCCCCCCACCCGGCGCGGGCGCGGGGGCGGGGCGCCGCTTTTGCCCTGGCGGCCGAGCCAGTCGGGGGCGGGGGTCCGTTACAACAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTAcc                                                                                          <  5:122557/135‑1 (MQ=255)
                                     ggctgACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTGTCCTGGCGGGCGGGCCAGTGCGGGTGGGGGGGGAGGTTGAAGAACGCGGCGGGTTGCTGCAGTGGGCCGGCGGCTACCGgcagca                                                                                   >  1:303014/1‑135 (MQ=255)
                                     ggctgACGGCAGCCACAAGGCTCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCAGGGTCGGGGGTCAGGTATAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGGTACCGgccgca                                                                                   >  6:18947/1‑135 (MQ=255)
                                                              gAGCGTGGGCGGGGCGCCGCTTTTGTCCTTGCGGCCGAGCCAGTCAGGGTCGGGGGTCAGGTAGAATAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGt                                                          >  5:5329/1‑135 (MQ=255)
                                                                  ggggggggggcgCGGTTTTGGCCCGGGGGGCCGCGCCAGGCGGGGGGGGGGGTCTGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCAc                                                      <  5:220998/133‑1 (MQ=255)
                                                                  gggggcggggcgCCGCTTTTGTCCTGGCGGCCGAGCCAGGCCGGGGCGGGGGGCAGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCAc                                                      <  7:193594/133‑1 (MQ=255)
                                                                             gccgcTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGCCGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCGTGGTGCACTCGTGGTCGAg                                           >  1:110654/1‑135 (MQ=255)
                                                                              ccgcTTTTGTCCAGGCGGCCGCGCCAGTCCGGGTCGGGGGTCAGGTATAAGAAAGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCCGCAGCACCCGGCGCGGCCGCCCCCCCATGGGGCCCTCCGGGTcccgc                                          >  6:254157/1‑131 (MQ=255)
                                                                                 cTTTGGGCCGGGCGCCCGCGCCAGGCGGGGGGGGGGGGCTGGTACAACCCCGCGGCGGGTGCCGGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTGGGTGCACTCGTGGTCGAGCACg                                       <  4:195008/135‑1 (MQ=255)
                                                                                         cgggcggcCGAGCCAGGCCGGGGGGGGGGGCAGGTAGAAGACCGCGGCGGGGTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTc                               <  2:198134/133‑1 (MQ=255)
                                                                                              ggcCGCGCCTGGCGGGGGCGGGGGCCTGGTCGCACCCCGCGGCGGGTGCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGGCGAGCACGGCCAGGTCGGTGa                          <  1:225273/135‑1 (MQ=255)
                                                                                                         ccgggggCGGGGGCCAGGTACACCCCCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAg               <  3:221288/132‑1 (MQ=255)
                                                                                                          ccGGGGCGGGGGGCAGGTCGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGAGCGAgg              <  2:218722/135‑1 (MQ=255)
                                                                                                            ggggCGGGGGTCCGGGAGAAACCCGCGGCGGGTGCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTGGGTGCACTCGGGGGCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGc            <  3:151770/135‑1 (MQ=255)
                                                                                                                cGGGGGTCCGGTAGAAGAACGCGGCGGGTGCCTGCCGGGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGCAACg        <  7:243848/135‑1 (MQ=255)
                                                                                                                      tCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGCAACGcctgtc  >  5:95877/1‑130 (MQ=255)
                                                                                                                      cgtgttAGAAGCCCGCGGCGGGTGCCGGCTGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGGCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGCAACGCATAGa  <  2:303014/130‑1 (MQ=255)
                                                                                                                                  |                                                                                                                          
CTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGCAACGCATAGA  >  KT2440/1720840‑1721092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑