Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,721,870	A→C	36.4%	V98G (GTC→GGC)	CJ019_01617 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,721,870	0	A	C	36.4%	13.9 / 2.3	30	V98G (GTC→GGC)	CJ019_01617	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (16/2);  new base C (8/2);  total (26/4)
Fisher's exact test for biased strand distribution p-value = 6.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCT  >  KT2440/1721741‑1721992                                                                                                                                  |                                                                                                                           tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCGGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGCCCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGAccccg                                                                                                                       >  8:217002/1‑133 (MQ=255) tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTGCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATAGTGGTCCCCGTCTTACTCGGCCCAg                                                                                                                       >  2:326616/1‑135 (MQ=255) tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGCTGCGGCCCCCGCGGGCAGCATCTTCTTCCCCGTCTTCCTCGCCCCAg                                                                                                                       >  3:259059/1‑135 (MQ=255) tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAg                                                                                                                       >  2:287465/1‑135 (MQ=255) tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCCGCGCTGATGCGGACCCAGCGGGCACCATCTTAATCCCCGGATTCCTCGcccccc                                                                                                                       >  5:14133/1‑133 (MQ=255)        tAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGGCCCAGCGGTCAGCATCTTCTTCCCCGGCTTCCTCGCCCCAGCCACCCg                                                                                                                >  7:167184/1‑135 (MQ=255)          aaCCAATTCATAAATAAACGAGCAAGCGTGCGGTTTAATTATGTACGCCAGGTTATTTCCTCTTCCCCGTCGGCGCTGATGCGGACCCAGCGGTCAGAATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGgc                                                                                                              <  2:1279/135‑1 (MQ=255)                         aaaCGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCCGCCCCCCGGCGCGCAGCGCCCTTcc                                                                                               >  6:152645/1‑135 (MQ=255)                           aCGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGGCTTCCTCGACCCAGCCCCCCCGCGCGCAGCGCACTTCcac                                                                                             >  6:124973/1‑135 (MQ=255)                                 aaGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGCCCCAGCGGGCAGCCTCTTCGTCCCCGTCTTCCTCGCCCCAGCCACCCGGCGCGCCGCGCCCGTcccccccccg                                                                                       >  4:25644/1‑133 (MQ=255)                                 aaGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCCGCGGGGCGCCTCTTCGTCCCCGTCGTCCTCGACCCCCCCCCCCCGCGCCCCGCGCCCATCCACCCCCac                                                                                       >  2:111285/1‑134 (MQ=255)                                 aaGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGCCCCAGCCACCCGGCGCGCAGCGCACTTCCCCCCCCAg                                                                                       >  4:127903/1‑135 (MQ=255)                                 aaGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCCCCCGGCGCGCAGCGCACTTCCACcccccg                                                                                       >  8:251367/1‑133 (MQ=255)                                 aaGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTAGCCCCAGCCACCCGGCGCCCAGCGCCCTTACACACCCac                                                                                       >  1:7143/1‑134 (MQ=255)                                 aaGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCAACTTATTCCCCGGCGTCCGCGCACCAGCCACCCGGCGCGCCCCGCACGTACACACCCac                                                                                       >  5:333682/1‑134 (MQ=255)                                      tGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCAGATGCGGACCCCGCGGGCCGCATCGTCTTCCCCGGCTTCCGCGGCCCCGCCCCCCGGCGCGCCGCGCCCGTCCCCACCCCgcgccg                                                                                  >  1:77375/1‑133 (MQ=255)                                           tttAATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCCCCGGTCCGCATCTTCTTCCCCCTCTTCCTCGACCCCGCCACCCCGCGCGCAGCGCCCATCCACACCCACCGCGGCcaaa                                                                             >  6:36836/1‑134 (MQ=255)                                                tgatgTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCCTCTTCTTCCCCGTCGTCCTCGCCCCAGCCCCCCGGCGCGCCGCGCAAATCCACACCCCGCCCCGCCAATgcggc                                                                        >  5:158950/1‑135 (MQ=255)                                                                aTTTCCTCTTCACCGTCTGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTACTCGACCCAGCCCCCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAAcc                                                        >  6:80186/1‑134 (MQ=255)                                                                aTTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGGCAGCAACTTCTTCCCCGTCTTCCGCGCCCCAGCCCCCCGGCGCGCAGCGCACTTACACACCCAGCGCGCCCAATGCGGCGCCGGCGCAGGCCACg                                                        >  6:75389/1‑135 (MQ=255)                                                                                  gcgcTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCCCGGCGTc                                      >  1:13280/1‑135 (MQ=255)                                                                                     cTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCCCGGCGTCTgg                                   >  8:66803/1‑135 (MQ=255)                                                                                                cccgCGGCCAGAATCTTCTCCCCCGGCTCCCCCCCCCCACCCCCCCGGCGCCCCCCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAg                        <  4:259059/132‑1 (MQ=255)                                                                                                ccAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCACCGTCATCGGCCCACGGCGTCTGGGCGCgtt                            >  4:274417/1‑131 (MQ=255)                                                                                                   gCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGAt                      >  4:322832/1‑134 (MQ=255)                                                                                                    cGGTCTGCATGTTATCCCCCGGCTCGCGCGACCCAGCCCCCGGCCCCCCCGCCCACTTCCACAGCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATAc                    <  4:307908/135‑1 (MQ=255)                                                                                                     ggTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACa                   >  4:299043/1‑135 (MQ=255)                                                                                                     ggTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACa                   >  2:214777/1‑135 (MQ=255)                                                                                                      gTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCCGGCAACGTCATCGGCCCACGGCGGCGGGTCGCGGTGCAGATACac                  >  3:6070/1‑134 (MQ=255)                                                                                                       acctcATCTTCTCCCCGGGCTGCCCCCCCCCCGCCACCGGGGGCGCCGCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAgg                 <  3:144603/131‑1 (MQ=255)                                                                                                                        gTCTTCCTCGACCCAGCCACCCGGCGCGCAGCCCACTTCCACACCCAGCGCCGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGCCGTCTGGTCGCTTTACATATACAGGCTGTTCCATTTACCt  >  6:172272/1‑133 (MQ=255)                                                                                                                                  |                                                                                                                           TCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCT  >  KT2440/1721741‑1721992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑