| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,807,474 | G→A | 100% | G258D (GGC→GAC) | yeiG → | S‑formylglutathione hydrolase YeiG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,807,474 | 0 | G | A | 97.0% | 104.7 / ‑4.0 | 33 | G258D (GGC→GAC) | yeiG | S‑formylglutathione hydrolase YeiG |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (12/20); total (13/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGGCCAATGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCAC > KT2440/1807342‑1807596 | cAGGCCAATGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGCAGCGCTGGCTCAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCt > 7:192766/1‑135 (MQ=255) gTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGctacta > 1:270766/1‑135 (MQ=255) gTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGctacta < 1:78181/135‑1 (MQ=255) gTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGctacta > 3:161856/1‑135 (MQ=255) tggATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCAt < 5:77799/133‑1 (MQ=255) aGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCt > 3:254596/1‑135 (MQ=255) ggCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTc > 6:282614/1‑135 (MQ=255) aCCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCg < 6:308566/135‑1 (MQ=255) cGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCgag < 6:138703/135‑1 (MQ=255) gacTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCAc < 6:90502/135‑1 (MQ=255) agaAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCca < 4:75642/133‑1 (MQ=255) cTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTg < 6:250750/135‑1 (MQ=255) cTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTg > 4:23665/1‑135 (MQ=255) aGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGc < 3:23665/133‑1 (MQ=255) ccTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTg < 7:327596/135‑1 (MQ=255) cgcTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCggg > 8:103053/1‑135 (MQ=255) cgcTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCggg > 7:74808/1‑135 (MQ=255) gAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTaa > 1:43775/1‑135 (MQ=255) ttcAGGCGGCTCGCAAAGGTGGGCTTGCTATGTCCCTGCGTATGCAGCCAGACTATGAACATAGCTACTACTTGATTGCCAGCTTGATCGAGGAGCACCTGCGCCATCATGCGGTGGCTCTGGGGCGGGTTTTAc < 8:110001/133‑1 (MQ=255) aaCAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTa < 1:104540/133‑1 (MQ=255) cggcACGCAAAGGTGGCCATGCAATGACCGTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGcctcc < 2:137282/135‑1 (MQ=255) cggcACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGcctcc < 2:132983/135‑1 (MQ=255) aCGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCtgtg < 8:263742/135‑1 (MQ=255) aCGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATAGACAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGAGGCACTGGGGCGGGTTTAACGCCTCCtgtg > 2:142377/1‑135 (MQ=255) ggCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCCTCGCGCAGCACCTGCGCCATCATGCGGTGGCACGGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCg < 7:51401/135‑1 (MQ=255) ccATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTg > 8:168963/1‑135 (MQ=255) tGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGc > 1:118280/1‑40 (MQ=255) tGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGc < 2:118280/40‑1 (MQ=255) tGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGccc < 2:270766/135‑1 (MQ=255) gTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGcac < 3:55521/135‑1 (MQ=255) gTAGGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGGGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGcac < 6:63755/135‑1 (MQ=255) ccAGACTATGCCCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATg < 3:24777/64‑1 (MQ=255) ccAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATg > 4:24777/1‑64 (MQ=255) | CAGGCCAATGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCAC > KT2440/1807342‑1807596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |