Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,869,832	G→A	6.3%	L197L (TTG→TTA)	CJ019_01787 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,869,832	0	G	A	6.3%	203.7 / 3.9	63	L197L (TTG→TTA)	CJ019_01787	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/33);  new base A (2/2);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CCGGCCATCGCGGCCTACAACCTGATCAATGAGCCAGCACCGGAGAAACAGGGTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGT  >  KT2440/1869698‑1869965                                                                                                                                       |                                                                                                                                      ccGGCCATCGCGGCCTACAACCTGATCAATGAGCCAGCACCGGAGAAACAGGGTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTg                                                                                                                                       >  4:197348/1‑135 (MQ=255)          gcgGCCTACAACCTGATCAATGAGCAAGCACCGGAGAATCAGGGGGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGGAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCgg                                                                                                                              <  3:129393/135‑1 (MQ=255)                          tCAATGAGCCAGCACCGGAGAAACAGGGTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCa                                                                                                              >  1:246552/1‑135 (MQ=255)                           cAATGAGCCAGCACCGGAGAAACAGGGTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCGGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCAt                                                                                                             >  6:211214/1‑135 (MQ=255)                            aaTGAGCCAGCACCGGAGAAACAGGGTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTACTGTACCGGCAACTGATAGCGGCCATc                                                                                                            >  7:41203/1‑135 (MQ=255)                            aaTGAGCAAGAACCGGAGAAACAGGGTGGCCTGGCTGAACATGCAGAACTCGGGCAGATGAAGCAGTGGTATTCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTACTGTACCTGAAACTGATAGCGTCCa                                                                                                              >  7:35837/1‑133 (MQ=255)                             aTGCGCCAGCACCGGAGAAACAGGGTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATcc                                                                                                           <  2:246552/135‑1 (MQ=255)                                    aGCCCCGGAGAAACAGGGTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCgaaga                                                                                                    <  8:24323/135‑1 (MQ=255)                                    aGCACCGGAGAAACAGGGTGGCCTGGCTGAACATGCCGAACCCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCgaaga                                                                                                    <  4:143702/135‑1 (MQ=255)                                         cGGAGAAACAGGGTGGCCTGGCTGAACTTGCCTAACTCGGGCAGATGCAGCAGTGTTATGCGCAAGCGGAGGGCGGTGCGCGCTATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACg                                                                                               <  3:256030/135‑1 (MQ=255)                                         cGGAGAAACAGGGTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACg                                                                                               <  3:66536/135‑1 (MQ=255)                                              aaaCAGGTTGGCCTGTCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCTCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGAt                                                                                          >  2:253896/1‑135 (MQ=255)                                              aaaCAGGGTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGAt                                                                                          >  7:178090/1‑135 (MQ=255)                                                    ggTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCg                                                                                    <  4:170172/135‑1 (MQ=255)                                                    ggTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTAGGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCg                                                                                    <  5:229351/135‑1 (MQ=255)                                                     gTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGa                                                                                   <  4:223817/135‑1 (MQ=255)                                                      tGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGa                                                                                   >  5:90043/1‑134 (MQ=255)                                                                   cATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCTCTAGCGCAGGGGGGTGCGCGCGTTCTGCCCTTGCTGTACAGGAAACTGATATCGGCCATCCGCTAAGAAGACGCTGATACGCCGATCATGGTCGATGcc                                                                     <  1:253896/135‑1 (MQ=255)                                                                   cATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGcc                                                                     <  5:322677/135‑1 (MQ=255)                                                                     tGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCgg                                                                   <  5:111245/135‑1 (MQ=255)                                                                          aaCTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGt                                                              >  8:96415/1‑135 (MQ=255)                                                                          aaCTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGt                                                              >  4:116869/1‑135 (MQ=255)                                                                          aaCTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGt                                                              >  2:158924/1‑135 (MQ=255)                                                                           aCTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTa                                                             <  4:201832/135‑1 (MQ=255)                                                                                        cagcagTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGAc                                                >  7:15869/1‑135 (MQ=255)                                                                                          gcagTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCGCTTGCTGTACCGGCAACTGATAGCGGCCTTCGGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGAcgc                                              <  1:216279/135‑1 (MQ=255)                                                                                              tGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTc                                          >  4:305849/1‑135 (MQ=255)                                                                                                 tATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCgg                                        <  3:116869/134‑1 (MQ=255)                                                                                                   tGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTa                                     >  6:37669/1‑135 (MQ=255)                                                                                                   tGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTa                                     >  8:304640/1‑135 (MQ=255)                                                                                                   tGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTAGGATa                                     >  7:240961/1‑135 (MQ=255)                                                                                                      gcAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTg                                  >  7:227666/1‑135 (MQ=255)                                                                                                        acgcgcCGGGGGGTGCGCGCGATCTGCCCTTGCTGGACCGCCAACTGTTAGCGGCCATCCGGGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGc                                <  2:310820/133‑1 (MQ=255)                                                                                                        aaGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGc                                >  5:305136/1‑135 (MQ=255)                                                                                                        aaGCGCAGGGCGGTGCGCGCGATCTGCCCGTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGc                                <  6:167709/135‑1 (MQ=255)                                                                                                         aGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGcc                               <  8:12153/135‑1 (MQ=255)                                                                                                           cgcAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCgg                             >  7:88288/1‑135 (MQ=255)                                                                                                           cgcAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCgg                             >  7:93050/1‑135 (MQ=255)                                                                                                           cgcAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCgg                             >  2:328372/1‑135 (MQ=255)                                                                                                           cgcAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCgg                             >  3:267139/1‑135 (MQ=255)                                                                                                             cAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGcc                                                                     >  7:261017/1‑93 (MQ=255)                                                                                                             cAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGcc                                                                     <  8:261017/93‑1 (MQ=255)                                                                                                             cAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGgcg                           >  7:178739/1‑135 (MQ=255)                                                                                                              aGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGgcgc                          >  3:237193/1‑135 (MQ=255)                                                                                                                   ggTGCGCGGGATCTGCCCTGGCTTGACCGGCAACTGATCGCTGCCATCCGCTAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTTGGATCCTGGCGCAGACGCGTTCGGCTAATGGCCGGCGCCTCTg                     <  4:156900/135‑1 (MQ=255)                                                                                                                   ggTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATCGCGGCCAGCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTCCTGGCCGGCGCCGCTg                     <  4:156891/135‑1 (MQ=255)                                                                                                                   ggTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCt                      >  2:148513/1‑134 (MQ=255)                                                                                                                        gcgcgATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGa                >  4:61706/1‑135 (MQ=255)                                                                                                                        gcgcgATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGa                >  4:29815/1‑135 (MQ=255)                                                                                                                        gcgcgATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCGGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAgc                >  5:274716/1‑134 (MQ=255)                                                                                                                          gcgATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGAcc              <  2:214245/135‑1 (MQ=255)                                                                                                                          gcgATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGAcc              <  4:300449/135‑1 (MQ=255)                                                                                                                          gcgATCTGCCCTTGCTGGACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGGTCGGCTACTGGCCGGCGCCGCTGGAAGAcc              <  2:37872/135‑1 (MQ=255)                                                                                                                          gagATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGAGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGAcc              <  7:90520/133‑1 (MQ=255)                                                                                                                            gataggCCCTTAATGTACCGGCAACTGATATCGCACATCATCTAAGAAGACTCTTATACGACGATCATGGTCTATGCCTGCTGGTATGAAGGCGCATACTCGTTCTGCTACTGGCCGGCGCCGCTGGAAGACCcg            <  8:35837/130‑1 (MQ=255)                                                                                                                            gATCTGCCCTTGCTGTACCGGCAACTGATCGCGGCCATCCGCTAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCcg            <  5:211214/135‑1 (MQ=255)                                                                                                                            gATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCcg            <  8:98484/135‑1 (MQ=255)                                                                                                                            gATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCcg            <  6:305136/135‑1 (MQ=255)                                                                                                                            gATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCcg            <  6:200555/135‑1 (MQ=255)                                                                                                                            gATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCcg            <  3:197348/135‑1 (MQ=255)                                                                                                                            gATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCcg            <  3:29815/135‑1 (MQ=255)                                                                                                                            gATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCcg            <  3:61706/135‑1 (MQ=255)                                                                                                                            gATCTGCCCTTACTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGTTGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGGTACTGGCCGGCGCCGCTGGAAGACCcg            <  8:41203/135‑1 (MQ=255)                                                                                                                                   ccTGGCTGTACCGGCAACTGATAGCGGCCATCCGCTAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGt       <  6:38285/133‑1 (MQ=255)                                                                                                                                       gCTGTACCGTCAACTGATAGCGGCCATCCGAGAAGAAGACGCTTATACGCCGATCATGGTCTATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGt  <  8:240961/134‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      CCGGCCATCGCGGCCTACAACCTGATCAATGAGCCAGCACCGGAGAAACAGGGTGGCCTGGCTGAACATGCCGAACTCGGGCAGATGCAGCAGTGGTATGCGCAAGCGCAGGGCGGTGCGCGCGATCTGCCCTTGCTGTACCGGCAACTGATAGCGGCCATCCGCGAAGAAGACGCTGATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGT  >  KT2440/1869698‑1869965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑