Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,900,316 A→C 26.6% V160G (GTC→GGC)  CJ019_01816 ← 2‑ketogluconate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,900,3160AC26.6% 94.8 / 4.7 72V160G (GTC→GGC) CJ019_018162‑ketogluconate reductase
Reads supporting (aligned to +/- strand):  ref base A (29/21);  new base C (19/2);  total (49/23)
Fisher's exact test for biased strand distribution p-value = 1.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGCATCGCTGTCCTTGGCCAACTGCAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGTCACCGGTGCAGATCCCCC  >  KT2440/1900187‑1900445
                                                                                                                                 |                                                                                                                                 
aGCGCATCGCTGTCCTTGGCCAACTGCAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGCAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCgg                                                                                                                              >  6:256706/1‑135 (MQ=255)
   gcATCGCTGTCCTTGGCCAACTGAAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGccc                                                                                                                           <  5:276020/135‑1 (MQ=255)
     aTCGCTGTCCTTGGCCAACTGCAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCAt                                                                                                                         >  1:17368/1‑135 (MQ=255)
       cGCTGTCCTTGGCCAACTGCAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTCCCGCCCGCCCGACCCGGCCCATGc                                                                                                                       >  7:264988/1‑135 (MQ=255)
       cGCTGTCCTTGGCCAACTGCAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGc                                                                                                                       <  7:13980/135‑1 (MQ=255)
         cTGTCCTTGGCCAACTGCAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCcg                                                                                                                     <  2:114203/135‑1 (MQ=255)
                    cAACTGCAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCATGCCGACCAAGCCCAc                                                                                                          >  2:258238/1‑135 (MQ=255)
                    cAACTGCAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCAc                                                                                                          >  7:233733/1‑135 (MQ=255)
                     aaCTGCAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCAcg                                                                                                         <  8:84976/135‑1 (MQ=255)
                     aaCTGCAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGCTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCAcg                                                                                                         <  4:327311/135‑1 (MQ=255)
                      aCTGCAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGAAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCAcgc                                                                                                        <  7:225170/135‑1 (MQ=255)
                        tGCAGCAGATCTGCGTCGAACCCGTAGTGCACATCCAGTGCCTGTAGATCTGTTTAGCGGATCGGGCAACCGAAGGCTGCGGCCCGCTGTGCTACCGCCCGCCAGACCCGGCCAATGCCGACGATGCCCAcgc                                                                                                        <  4:220527/133‑1 (MQ=255)
                         gcagcaGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCCCCCGCCCCGGCCCCAGCCGCCGAAGCCCAcccg                                                                                                       >  3:207681/1‑133 (MQ=255)
                         gcagcaGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGACCGCCCGACCCCGCCCATGCCGACCATGCCCACCCGCa                                                                                                     >  2:239734/1‑135 (MQ=255)
                           agcagATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCCCCCCCGCCCAAGCCGCCGCTGCCCCcgcccctg                                                                                                   >  2:297159/1‑130 (MQ=255)
                              agATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGccc                                                                                                <  7:281147/135‑1 (MQ=255)
                               gATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCg                                                                                               <  8:34735/135‑1 (MQ=255)
                                   tGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCATGCCGCCGATGCCCACGCGCATGCCCGaccc                                                                                           >  4:203516/1‑133 (MQ=255)
                                     ccTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGCCGATGCCCACGCGCATGCCCGACACCt                                                                                         >  6:115777/1‑135 (MQ=255)
                                       tCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGCCGATGCCCACGCGCATGCCCGACACCTGc                                                                                       >  3:34564/1‑135 (MQ=255)
                                        cGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCATGCCGACGAAGCCCACGCGCATGCCCGACACCTGcc                                                                                      >  1:190097/1‑135 (MQ=255)
                                        cGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCAGGCCGACGATGCCCACGCGCATGCCCGACACCTGcc                                                                                      >  1:103887/1‑135 (MQ=255)
                                             ccGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCCCCCGCCCCGGCCCAAGCCCAAGAAGCCCACCCGCAAGCCCCACACCTCCCCGGcc                                                                                 >  6:37915/1‑135 (MQ=255)
                                                        aTCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCgg                                                                      >  5:144353/1‑135 (MQ=255)
                                                          ccAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTg                                                                    >  6:181565/1‑135 (MQ=255)
                                                          ccAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCCGCCCATGCCGCCGATGCCCACGCGCCTGCCCGACCCCTGCCGGGGCAGTGGCAGCGGTg                                                                    >  1:295040/1‑135 (MQ=255)
                                                           cAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGCCACCTGCCGGGCCCGTGGCAGCGGTGc                                                                   >  8:236356/1‑135 (MQ=255)
                                                            aGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCATGCCGACGCTGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTg                                                                    >  1:263859/1‑133 (MQ=255)
                                                                ccTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCg                                                                                                                               <  6:275333/70‑1 (MQ=255)
                                                                ccTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCg                                                                                                                               >  5:275333/1‑70 (MQ=255)
                                                                 cTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGCCGATGCCCCCGCGCATGCCCGACACCTGCCGGGCCAGGGGCTGCGGTGCCAgcgg                                                             >  6:279953/1‑135 (MQ=255)
                                                                 cTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCATCTGTGCTACCGCCCCCCCGACCCGGCCAATGCCCACGAAGCCCACGCGCCTGCCCGACCCCTGCCGTGCCAGTCGCAAGGGTGCCAgcgg                                                             >  7:70515/1‑135 (MQ=255)
                                                                     aGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCCTGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCgtg                                                         >  6:184237/1‑135 (MQ=255)
                                                                     aGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCCCCCGACCCGGCCCCTGCCGACGATGCCCCCGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCgtg                                                         >  3:256458/1‑135 (MQ=255)
                                                                      gATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCgtgg                                                        >  7:185859/1‑135 (MQ=255)
                                                                       aTCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCCTGCCGACGATGCCCCCGCGCATGCCCGCCACCTGCCGGGCCAGTGGCAGCGGTGCCCGCGGCggggt                                                       >  1:291779/1‑135 (MQ=255)
                                                                       aTCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCCGCCCATGCCGACGCTGCCCCCGCGCCTGCCCGACACCAGCCCGGCCAGTGGCAGCGGTGCCAGCCGCgtggt                                                       >  7:245998/1‑135 (MQ=255)
                                                                       aTCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCCACCCCTCCCCTGCCCACGATGCCCCCGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCCGCGGCgtggt                                                       >  4:76059/1‑135 (MQ=255)
                                                                         cGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGTCAGCGGTGCCAGCGGCGTGGTGc                                                     <  1:239734/135‑1 (MQ=255)
                                                                           gtgtAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCtg                                                   >  4:308745/1‑135 (MQ=255)
                                                                               aGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCCTGCCGACGATGCCCCCGCGCCTGCCCGACACCTGCCGGGCCCGTGGCAGCGGTGCCAGCCGCGTGGGGCtggtgg                                               >  7:273337/1‑135 (MQ=255)
                                                                               aGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCATGGCGACCAATCCCACCCGCATTCCCGCCACCTGCCGGGCCAGTGGCAGCGGTGCCCGCGGCGTGGTGCtggtgg                                               >  8:19324/1‑135 (MQ=255)
                                                                               aGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCATGCCGCCGATGCCCACCCGCCTGCCCGCCCCCGGCCCGGCCAGGGGCCGCGGGGCCAGCGGcggggggcgggggg                                               >  5:46057/1‑135 (MQ=255)
                                                                               aGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGCCGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCCGTGCCAGCGGCGTGGTGCtggtgg                                               >  6:59236/1‑135 (MQ=255)
                                                                               aGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCtggtgg                                               >  7:328855/1‑135 (MQ=255)
                                                                               aGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCtggtgg                                               <  2:96762/135‑1 (MQ=255)
                                                                                 cGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCCTGCCGCCGATGCCCCCGCGCATGCCCGCCACCTGCCGGGCCAGTGGCAGCGGTGCCCGCGGCGTGGTGCtggtgggc                                             >  7:316238/1‑133 (MQ=255)
                                                                                         gCAACCGAAGGTTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGCCGATGCCCACGCGCAAGCCCGCCCCCGGCCCGGCCACTGGCCGCGGTGCCAGCGGCGGGGGGCTGGTGGCCCCAcggcc                                     >  4:56345/1‑135 (MQ=255)
                                                                                         gCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCCTGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAAcggcc                                     >  6:174688/1‑135 (MQ=255)
                                                                                         gCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGGCGATGCCCACGCGCATGCCCGACCCCTGCCGGGCCAGTGGCAGCGGTGCCCGCGGCGTGGTGCTGGTGGCCCACcggcc                                     >  6:252647/1‑135 (MQ=255)
                                                                                         gCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAAcggcc                                     >  1:37243/1‑135 (MQ=255)
                                                                                         gCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGAAGCCCACGCGCATTACCGACACCTGCCGGGCCAGTGGCATCGGTGCCAGCGGCGTGGTGCTGGTGGCCCCAcggcc                                     >  7:205707/1‑135 (MQ=255)
                                                                                         gCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCCGCCCATGGCGGCGATGCCCCCGCGCCTTCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGGGGTGGTGGTGGCCCCAcggcc                                     >  5:210015/1‑135 (MQ=255)
                                                                                         gCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCCCCCCACCCGGCCCCAGCCGACGATGCCCACGCGCAACCCCGACACCTGCCGGGCCAGTGGAAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCACcggcc                                     >  1:258528/1‑135 (MQ=255)
                                                                                         gCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCCCCCCACCCCGCCCCTGCCGCCCATGCCCACCCGCAAGCCCGCCCCCTGCCGGGCCACTGGCAGCGGTGCCAGCGGCGGGGTGCTGGTGGCCCCAccgcc                                     >  6:277688/1‑135 (MQ=255)
                                                                                         gCAACCGAAGGCTGCCGCCCGCTGTGCTACAGCCCGCCCGCCCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGCCACCTGCCGGGCCAGTGGCAGCGGTGCCCGCGGCGGGGTGCTGGTGGCCCCGcggcc                                     >  3:9374/1‑135 (MQ=255)
                                                                                                 aGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGcgaa                             >  8:111069/1‑135 (MQ=255)
                                                                                                   gCTGCCGCCCGCTGTGCTACCGCCCGCCCGGCCCCGCCCCTGCCGGCGCTGCCCCCGCGCCTGCCCGCCCCCTGCCGGGGCCGGGGGAGCGGTGCCCCCGGCGGGGGGGGGGTGGGCCCCCCGCCcgcgcggccg                           >  6:209614/1‑131 (MQ=255)
                                                                                                     tGCCGCCCGCTGTGCTACCGCCCGCCCGCCCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGcgaacgaa                         <  5:181565/135‑1 (MQ=255)
                                                                                                         gcccgcTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCgg                     <  3:203516/135‑1 (MQ=255)
                                                                                                          cccgcTGTGCTACCGCCCGCCCGCCCCGGCCCCTGCCGCCGATGCCCCCGCGCATGCCCGCCACCAGCCGGGCCAGGGGCAGCGGTGCCAGCGGCGTGGGGGGGGTGGCCCCCCGGCCGGCGCGCACGAAGCGGt                    >  4:183319/1‑135 (MQ=255)
                                                                                                          cccgcTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGCACGAAGCgg                     >  6:136950/1‑134 (MQ=255)
                                                                                                          cccgcTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGt                    <  5:74241/135‑1 (MQ=255)
                                                                                                          cccgcTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGt                    <  8:233733/135‑1 (MQ=255)
                                                                                                          cccgcTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGt                    <  8:328855/135‑1 (MQ=255)
                                                                                                          cccgcTGTGCTACCGCCCGCCCGACCCGGCCCATCCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGt                    <  3:205091/135‑1 (MQ=255)
                                                                                                          cccgcGGGGCTCCCGCCCGCCCGCCCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGt                    <  3:219525/135‑1 (MQ=255)
                                                                                                                gtgCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGTCACCgg              <  2:291779/135‑1 (MQ=255)
                                                                                                                 tgCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGTCACCGGt             <  4:82676/135‑1 (MQ=255)
                                                                                                                       cgcccgccCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACCCCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGTCACCGGTGCAGAt       <  3:56345/135‑1 (MQ=255)
                                                                                                                       cgcccgccCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGTCACCGGTGCAGAt       <  5:174688/135‑1 (MQ=255)
                                                                                                                        gcccgcccgcccCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGTCACCGGTGCAGATc      <  1:309376/135‑1 (MQ=255)
                                                                                                                            gcccgACCCGGCCCATGCCGACGATGCCCCCGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGTCACCGGTGCAGATccccc  >  8:88055/1‑135 (MQ=255)
                                                                                                                                 |                                                                                                                                 
AGCGCATCGCTGTCCTTGGCCAACTGCAGCAGATCTGCCTCGAACCCGTAGGGCACATCCAGTGCCTGTAGATCGGTGTAGCGGATCGGGCAACCGAAGGCTGCCGCCCGCTGTGCTACCGCCCGCCCGACCCGGCCCATGCCGACGATGCCCACGCGCATGCCCGACACCTGCCGGGCCAGTGGCAGCGGTGCCAGCGGCGTGGTGCTGGTGGCCCAACGGCCGGCGCGAACGAAGCGGTCACCGGTGCAGATCCCCC  >  KT2440/1900187‑1900445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: