Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,923,103	C→T	7.1%	L15L (TTG→TTA)	CJ019_01837 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,923,103	0	C	T	7.1%	176.6 / 3.7	56	L15L (TTG→TTA)	CJ019_01837	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (16/36);  new base T (2/2);  total (18/38)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

CTGGTCGTTTTCGAGGTTCTTGATCACCTCGTCCAGCGACTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAGTGTAGAGGTTTATGTCGGCAGTTTCTCGAGGATCTTCCAGCAGGTGAGGCCGAAAATAC  >  KT2440/1922971‑1923235                                                                                                                                     |                                                                                                                                     cTGGTCGTTTTCGAGGTTCTTGATCACCTCGTCCAGCGACTTGCCCAGCGGCTCGGTTGCCTCTGGTTGCGCGGGCGCACTGCCGCCCCGCAGGCCAGGCAAGCCCGCCGCGTGTACCGGGGGAATGAAAAGCa                                                                                                                                     >  6:202525/1‑134 (MQ=255) cTGGTCGTTTTCGAGGTTCTTGATCACCTCGTCCAGCGACTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCa                                                                                                                                     <  5:11703/134‑1 (MQ=255) cTGGTCGTTTTCGAGGTTCTTGATCACCTCGTCCAGCGACTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCa                                                                                                                                     <  7:64928/134‑1 (MQ=255)  tGGTCGTTTTCGAGGTTCTTGATCACCTCGTCCAGCGCCTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCCCCGCTTGTACCGGGGCAATGAAAAGCa                                                                                                                                     >  5:82968/1‑133 (MQ=255)     tCGTTTTCGAGGTGCTTGATCACCTCGTCCAGCGACTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAagag                                                                                                                                 <  8:117065/134‑1 (MQ=255)       gTTTTCGAGGTTCTTGATCACCTCGTCCAGCGACTAGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCa                                                                                                                               >  5:117125/1‑134 (MQ=255)         tttCGAGGTTCTTGATCACCTCGTCCAGCGACTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCaaca                                                                                                                            <  7:12039/135‑1 (MQ=255)         tttCGAGGGTCTTGATCACCTCGTCCAGCGACTTGGCCTGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCaaca                                                                                                                            <  2:183778/135‑1 (MQ=255)              aGGGTCTTGATCACCTCGTCCAGCGACTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCaacaggaac                                                                                                                       <  1:9446/135‑1 (MQ=255)                              cGTCCAGCGACTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAACCCCCCCGCGTGTACCGGGGCAAAGAAAAGCAAGAGCAACACGAAAACCTAACCCAGGAggg                                                                                                       >  2:173988/1‑135 (MQ=255)                               gTCCAGCGACTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCaggagg                                                                                                        >  3:140844/1‑133 (MQ=255)                                 ccAGCGACTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGggcag                                                                                                    <  2:137057/135‑1 (MQ=255)                                   aGCGACTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCGTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACACGTAACGCAGGCGggcacgc                                                                                                  >  1:251677/1‑135 (MQ=255)                                      gccTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACt                                                                                               <  1:73635/133‑1 (MQ=255)                                            cccAGCGGCGCGGTGGCCGCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCa                                                                                           <  5:202525/133‑1 (MQ=255)                                                gCGGCTCGGTGGCCGCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTa                                                                                     <  6:82968/135‑1 (MQ=255)                                                        gTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGt                                                                             <  8:70055/135‑1 (MQ=255)                                                             ctctGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAAc                                                                        <  3:117284/135‑1 (MQ=255)                                                                 ggTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATc                                                                                       <  5:48343/116‑1 (MQ=255)                                                                 ggTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGa                                                                      <  4:293442/133‑1 (MQ=255)                                                                 ggTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAACAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATc                                                                                       >  6:48343/1‑116 (MQ=255)                                                                  gTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   <  2:202064/135‑1 (MQ=255)                                                                  gTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   <  3:234093/135‑1 (MQ=255)                                                                  gTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   <  1:173988/135‑1 (MQ=255)                                                                   ttGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   <  3:274325/134‑1 (MQ=255)                                                                   ttGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   <  4:140844/134‑1 (MQ=255)                                                                   ttGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   <  4:161564/134‑1 (MQ=255)                                                                   ttGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   <  6:66231/134‑1 (MQ=255)                                                                   ttGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   <  6:117125/134‑1 (MQ=255)                                                                   ttGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   <  8:134878/134‑1 (MQ=255)                                                                        cgGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   <  2:153095/129‑1 (MQ=255)                                                                        cgGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   >  1:153095/1‑129 (MQ=255)                                                                         gggCGCACTGCCTCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTATCGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAgt                                                            <  1:557/135‑1 (MQ=255)                                                                         gggCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   <  4:80304/128‑1 (MQ=255)                                                                         gggCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   >  3:80304/1‑128 (MQ=255)                                                                         gggCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAgt                                                            <  1:63595/135‑1 (MQ=255)                                                                         gggCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAgt                                                            <  2:114620/135‑1 (MQ=255)                                                                         gggCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAgt                                                            <  2:5757/135‑1 (MQ=255)                                                                         gggCGCACGGCCGCCGAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAgt                                                            <  7:163219/135‑1 (MQ=255)                                                                           gcgcACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGTAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAgt                                                            >  5:159599/1‑133 (MQ=255)                                                                           gcgcACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGTAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAgt                                                            >  5:153874/1‑133 (MQ=255)                                                                             gcACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGACCTGGCAAATCTACCTCAGGTCAAAGGATTGACCGAgtgt                                                          >  1:155154/1‑133 (MQ=255)                                                                                            aggccaggcAAGCCCGCCGCTGGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAGTGGAGAGGTTTATGTCGGCa                                         >  5:57693/1‑135 (MQ=255)                                                                                                   gcAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAGTGTAGAGGTTTATGTCGGCAGTTtctc                                  <  1:197823/135‑1 (MQ=255)                                                                                                      aGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAGTGTAGAGGTTTATGTCGGCAGTTTCTCGAg                               <  4:63592/135‑1 (MQ=255)                                                                                                        cccGCCGCTTGTACCGGGGCAATGAAAAGTAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAGTGTAGAGGTTTATGTCGGCAGTTTCTCGAGGa                             <  6:159599/135‑1 (MQ=255)                                                                                                        cccGCCGCTTGTACCGGGGCAATGAAAAGTAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAGTGTAGAGGTTTATGTCGGCAGTTTCTCGAGGa                             <  6:153874/135‑1 (MQ=255)                                                                                                           gccgcTTGTACCGGGGCAATGAAAAGCAAGAGCACCAGGAACAGGGAACGCAGGAGGGCCGGCCCTGGCAAATCTACCTCAGGTCAAACGATTGACCGAGTGTAGAGGTTTATGTCGGCAGTTTCTCGAGGATCt                          >  6:291041/1‑135 (MQ=255)                                                                                                           gccgcTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGG‑CAAACGATTGACCGAGTGTAGAGGTTTATGTCGGCAGTTTCTCGAGGATCtt                         >  4:18244/1‑135 (MQ=255)                                                                                                                       ggggCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAGTGTAGAGGTTTATGTCGGCAGTTTCTCGAGGATCTTCCAGCAGGTGa              <  1:181017/135‑1 (MQ=255)                                                                                                                                aaaaGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   <  7:310448/73‑1 (MQ=255)                                                                                                                                aaaaGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTg                                                                   >  8:310448/1‑73 (MQ=255)                                                                                                                                aaaaGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCTAGTGTAGAGGTTTATGTCGGCAGTTTCTCTAGGATCTTCCAGCAGGTTAGGCCTaaa      >  6:96333/1‑134 (MQ=255)                                                                                                                                aaaaGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAGTGTAGAGGTTTATGTCGGCAGTTTCTCGAGGATCTTCCAGCAGGTGAGGCCGaa       >  7:178038/1‑133 (MQ=255)                                                                                                                                aaaaGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAGTGTAGAGGTTTATGTCGGCAGTTTCTCGAGGATCTTCCAGCAGGTGAGGCCGaa       <  8:178038/133‑1 (MQ=255)                                                                                                                                   aGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAGTGTAGAGGTTTATGTCGGCAGTTTCTCGAGGAGCTTCCAGCAGGTGAGGCCGAAAATAc  <  1:71936/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     CTGGTCGTTTTCGAGGTTCTTGATCACCTCGTCCAGCGACTTGCCCAGCGGCTCGGTGGCCTCTGGTTGCGCGGGCGCACTGCCGCCCAGCAGGCCAGGCAAGCCCGCCGCTTGTACCGGGGCAATGAAAAGCAAGAGCAACAGGAACAGGTAACGCAGGAGGGCAGGCACTGGCAAATCTACCTCAGGTCAAACGATTGACCGAGTGTAGAGGTTTATGTCGGCAGTTTCTCGAGGATCTTCCAGCAGGTGAGGCCGAAAATAC  >  KT2440/1922971‑1923235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑