| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 2,031,520 | G→T | 100% | T339T (ACC→ACA) | wecB ← | UDP‑N‑acetylglucosamine 2‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,031,520 | 0 | G | T | 96.1% | 86.3 / ‑3.2 | 26 | T339T (ACC→ACA) | wecB | UDP‑N‑acetylglucosamine 2‑epimerase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (18/7); total (19/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCC > KT2440/2031405‑2031649 | ggTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTAGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACaa > 8:234405/1‑135 (MQ=255) ggggggggCCAGGTGGCCCGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGAt > 2:267346/1‑135 (MQ=255) ggggggggCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCACATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAAACGAt > 8:299990/1‑135 (MQ=255) ggggggCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATg > 7:50343/1‑134 (MQ=255) gggCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTAGGGGCGAAGCGCATTTCGCGATACGCCGCCTCATCGGTCACCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGc < 3:306276/134‑1 (MQ=255) tttGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCtt > 4:216911/1‑135 (MQ=255) gTATGCGAGCGCAGCCTTTGCCATCGCCATATGTGAGATGGGCGAAGCACATTTCGCGATCCGCCGCCTCAGCGGTGAGCAAGGTACTCACCGCCCGCACAATCGATGCTTCTTCGGTCCCCACCAGCTTGccc > 7:215159/1‑134 (MQ=255) cgcgcgcAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCt > 4:161554/1‑135 (MQ=255) cgcAGGCTTTGCCAGCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTgccg < 1:267346/135‑1 (MQ=255) gcAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTgccgc > 7:308097/1‑135 (MQ=255) aGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCa > 4:173974/1‑135 (MQ=255) cATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTa > 8:182356/1‑90 (MQ=255) cATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTa < 7:182356/90‑1 (MQ=255) cATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTg > 8:227403/1‑135 (MQ=255) aTCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTgg < 7:299990/135‑1 (MQ=255) cATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCt > 1:7356/1‑135 (MQ=255) ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAAt < 1:264310/63‑1 (MQ=255) ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAAt > 2:264310/1‑63 (MQ=255) ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAg < 4:266038/87‑1 (MQ=255) ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAg > 3:266038/1‑87 (MQ=255) cTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCa > 3:83842/1‑133 (MQ=255) cTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCAcc > 5:299280/1‑135 (MQ=255) cTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCAcc > 1:121689/1‑135 (MQ=255) aCGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGc > 4:271650/1‑135 (MQ=255) ccTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAggg < 2:121689/135‑1 (MQ=255) aGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGCTTTGCCCAGGGCCGGCGCTtcc > 8:189090/1‑135 (MQ=255) | GGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCC > KT2440/2031405‑2031649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |